Gene:
Gene Symbol:
PPFIA1
Gene Name:
protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
Original Clone ID:
FLH186002.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HIP
HsCD00044464
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGATGTGCGAGGTGATGCCGACCATCAGCGAAGCAGAAGGCCCCCCTGGAGGAGGTGGA
GGCCATGGTTCCGGCTCCCCTTCACAGCCAGATGCAGATTCACATTTTGAACAGTTGATG
GTCTCCATGCTAGAAGAAAGGGACCGCCTTCTTGATACACTGAGAGAGACTCAAGAAACG
CTGGCCTTAACCCAGGGGAAGTTACACGAGGTTGGTCATGAAAGAGATTCCTTGCAGAGA
CAGCTCAACACAGCACTTCCACAGGAGTTCGCAGCACTTACTAAAGAACTCAATGTATGC
AGGGAACAGCTCCTTGAAAGGGAAGAAGAAATTGCTGAACTGAAAGCAGAAAGGAATAAC
ACCAGGCTGCTGTTAGAGCATTTGGAATGCCTTGTCTCCAGGCATGAGCGGTCTCTTAGG
ATGACCGTGGTGAAGAGACAAGCGCAGTCTCCAGCAGGCGTGTCCAGCGAAGTGGAAGTG
CTGAAAGCACTGAAGTCCTTATTTGAACACCACAAAGCTCTGGATGAAAAGGTGAGAGAG
CGATTACGAGTAGCACTTGAAAGATGTAGTTTGTTAGAAGAGGAATTAGGTGCCACACAC
AAAGAGCTAATGATTCTTAAAGAACAGAATAATCAGAAAAAAACTCTAACAGATGGAGTG
CTGGACATAAACCATGAACAAGAAAATACACCAAGCACGAGTGGAAAGAGATCTTCTGAT
GGTTCTTTAAGCCACGAGGAAGACCTTGCTAAAGTAATTGAGCTCCAAGAAATCATAAGT
AAGCAGTCAAGGGAACAGAGCCAAATGAAAGAACGCCTGGCTTCCCTTTCCAGTCATGTG
ACAGAACTGGAAGAGGATCTGGACACGGCTAGAAAAGATCTCATCAAATCTGAAGAAATG
AACACAAAATTGCAACGAGATGTCCGTGAAGCCATGGCCCAAAAGGAAGATATGGAAGAG
AGAATCACTACTCTTGAAAAACGCTACCTCGCTGCACAGCGTGAAGCCACATCTGTGCAT
GACCTCAATGATAAACTTGAAAATGAAATTGCAAATAAAGATTCTATGCATCGACAGACT
GAAGATAAAAACCGCCAGTTACAGGAGCGCTTGGAATTGGCAGAGCAAAAGCTGCAACAG
ACACTGAGGAAGGCAGAGACGCTCCCGGAGGTGGAGGCGGAGCTGGCCCAGAGGGTGGCA
GCGCTTTCCAAGGCTGAAGAGAGACACGGCAACATTGAAGAAAGGTTACGACAGATGGAA
GCACAGTTGGAGGAGAAGAATCAAGAACTGCAGCGGGCAAGGCAAAGAGAAAAAATGAAC
GAAGAACATAATAAACGTTTATCAGACACTGTTGACAAGCTGCTTTCAGAATCTAATGAG
AGGCTTCAACTTCATCTTAAAGAGAGAATGGCTGCTTTGGAAGATAAGAACTCTCTTTTA
AGAGAAGTTGAAAGTGCAAAAAAGCAGTTAGAAGGAACACAACACGATAAGGATCAGCTT
GTCCTAAACATTGAAGCACTGAGGGCTGAACTAGACCACATGAGACTAAGAGGTGCTTCA
CTTCATCATGGCCGACCCCACTTGGGCAGTGTCCCAGATTTCAGGTTCCCCATGGCAGAT
GGCCACACAGACTCCTACAGCACCAGTGCAGTGCTGCGGCGCACACAGAAAGGCCGGCTG
GCAGCCCTGCGAGATGAGCCTTCCAAGGTACAAACTCTTAATGAGCAGGATTGGGAACGT
GCCCAGCAAGCTAGTGTCTTGGCAAATGTAGCACAAGCATTCGAGAGTGATGCTGACGTG
TCTGATGGTGAAGATGACAGGGACACTCTCCTCAGCTCAGTTGACCTGCTATCGCCCAGC
GGGCAGGCCGACGCGCACACACTAGCCATGATGCTTCAGGAGCAGCTGGACGCCATCAAC
AAAGAGATCAGGTTGATTCAGGAAGAAAAAGAAAATACAGAGCAGCGGGCAGAGGAGATT
GAAAGTCGAGTTGGCAGTGGAAGTCTAGACAATCTTGGTCGTTTTAGATCAATGAGCTCC
ATTCCCCCCTACCCTGCTTCCTCGCTTGCTAGCTCCTCCCCTCCGGGCAGTGGGCGCTCC
ACCCCACGAAGGATCCCTCACAGCCCAGCTCGGGAAGTGGACAGACTGGGCGTCATGACC
CTTTTGCCACCTTCCAGAGAAGAGGTACGAGATGACAAGACAACCATAAAGTGTGAAACC
TCCCCGCCTTCCTCCCCGAGAGCCCTTCGGTTAGACCGGCTGCACAAAGGGGCGCTGCAC
ACCGTTAGCCACGAGGACATCAGGGACATAAGGAACTCCACAGGCTCCCAGGATGGTCCC
GTGAGCAACCCCAGCAGTAGCAACAGTAGCCAGGACTCGCTCCACAAAGCCCCAAAGAAG
AAAGGCATTAAGTCCTCCATTGGCCGCTTGTTTGGCAAGAAAGAAAAGGGCCGACCTGGA
CAAACTGGCAAAGAAGCATTAGGACAAGCTGGTGTTTCCGAGACGGATAACTCATCTCAG
GATGCCTTGGGACTTAGCAAATTGGGGGGACAGGCTGAAAAAAATCGTAAACTTCAAAAA
AAGCATGAATTGCTGGGGGAAGCCCGGAGACAAGGTTTACCTTTTGCCCAATGGGACGGG
CCAACGGTTGTGGTCTGGCTAGAGCTCTGGGTTGGGATGCCAGCCTGGTATGTGGCTGCC
TGCCGAGCAAACGTGAAAAGCGGGGCCATCATGTCGGCCCTGTCCGACACAGAGATCCAG
CGTGAGATTGGCATCAGCAACCCCCTGCACAGGCTGAAGCTGAGGCTGGCCATCCAGGAG
ATCATGTCGCTGACCAGCCCGTCTGCCCCGCCCACATCTAGAACGACACTCGCCTATGGG
GACATGAACCACGAGTGGATCGGCAACGAGTGGCTCCCCAGCCTGGGCCTCCCCCAGTAC
CGCAGCTACTTCATGGAGTGCCTTGTAGACGCCAGGATGCTGGACCACTTGACCAAGAAA
GACCTTCGAGGGCAGCTGAAAATGGTCGACAGTTTTCACAGAAACAGTTTCCAGTGTGGA
ATTATGTGCCTGAGAAGGTTAAATTATGACCGGAAAGAACTGGAAAGAAAAAGAGAAGAA
AGTCAGAGTGAAATAAAAGACGTGCTTGTTTGGAGCAATGATCGAGTGATTCGCTGGATC
CTGTCAATTGGCCTTAAAGAATATGCAAACAATCTTATAGAGAGTGGTGTTCACGGAGCA
CTTCTGGCCTTAGATGAAACCTTCGACTTCAGTGCACTGGCACTGCTGTTACAGATCCCA
ACGCAGAACACACAGGCTCGTGCTGTCTTGGAAAGAGAATTTAACAACCTTTTGGTCATG
GGGACTGATAGAAGGTTTGATGAAGATGATGATAAAAGCTTTAGGAGAGCACCTTCATGG
AGAAAAAAGTTTAGACCAAAGGACATTCGTGGCTTAGCTGCTGGGTCAGCAGAGACTCTC
CCTGCAAACTTCCGGGTGACTTCTTCTATGTCTTCCCCCTCTATGCAGCCAAAGAAGATG
CAGATGGACGGCAATGTATCAGGAACACAGAGGTTGGATTCTGCTACAGTCAGGACTTAC
TCCTGCTAG
Protein sequence predicted based on nucleotide sequence
MMCEVMPTISEAEGPPGGGGGHGSGSPSQPDADSHFEQLMVSMLEERDRLLDTLRETQET LALTQGKLHEVGHERDSLQRQLNTALPQEFAALTKELNVCREQLLEREEEIAELKAERNN TRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRE RLRVALERCSLLEEELGATHKELMILKEQNNQKKTLTDGVLDINHEQENTPSTSGKRSSD GSLSHEEDLAKVIELQEIISKQSREQSQMKERLASLSSHVTELEEDLDTARKDLIKSEEM NTKLQRDVREAMAQKEDMEERITTLEKRYLAAQREATSVHDLNDKLENEIANKDSMHRQT EDKNRQLQERLELAEQKLQQTLRKAETLPEVEAELAQRVAALSKAEERHGNIEERLRQME AQLEEKNQELQRARQREKMNEEHNKRLSDTVDKLLSESNERLQLHLKERMAALEDKNSLL REVESAKKQLEGTQHDKDQLVLNIEALRAELDHMRLRGASLHHGRPHLGSVPDFRFPMAD GHTDSYSTSAVLRRTQKGRLAALRDEPSKVQTLNEQDWERAQQASVLANVAQAFESDADV SDGEDDRDTLLSSVDLLSPSGQADAHTLAMMLQEQLDAINKEIRLIQEEKENTEQRAEEI ESRVGSGSLDNLGRFRSMSSIPPYPASSLASSSPPGSGRSTPRRIPHSPAREVDRLGVMT LLPPSREEVRDDKTTIKCETSPPSSPRALRLDRLHKGALHTVSHEDIRDIRNSTGSQDGP VSNPSSSNSSQDSLHKAPKKKGIKSSIGRLFGKKEKGRPGQTGKEALGQAGVSETDNSSQ DALGLSKLGGQAEKNRKLQKKHELLGEARRQGLPFAQWDGPTVVVWLELWVGMPAWYVAA CRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEIMSLTSPSAPPTSRTTLAYG DMNHEWIGNEWLPSLGLPQYRSYFMECLVDARMLDHLTKKDLRGQLKMVDSFHRNSFQCG IMCLRRLNYDRKELERKREESQSEIKDVLVWSNDRVIRWILSIGLKEYANNLIESGVHGA LLALDETFDFSALALLLQIPTQNTQARAVLEREFNNLLVMGTDRRFDEDDDKSFRRAPSW RKKFRPKDIRGLAAGSAETLPANFRVTSSMSSPSMQPKKMQMDGNVSGTQRLDSATVRTY SC*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
179
End on reference sequence
3787
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
