SEMA4D (Homo sapiens) in pLX304

Explanation of Terms

Gene:
Gene Symbol:  SEMA4D
Gene Name:  sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Original Clone ID:
ccsbBroad304_07622
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLX304
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00445604
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2587nts
Open reading frame: 1 to 2587

ATGAGGATGTGCACCCCCATTAGGGGGCTGCTCATGGCCCTTGCAGTGATGTTTGGGACA
GCGATGGCATTTGCACCCATACCCCGGATCACCTGGGAGCACAGAGAGGTGCACCTGGTG
CAGTTTCATGAGCCAGACATCTACAACTACTCAGCCTTGCTGCTGAGCGAGGACAAGGAC
ACCTTGTACATAGGTGCCCGGGAGGCGGTCTTCGCTGTGAACGCACTCAACATCTCCGAG
AAGCAGCATGAGGTGTATTGGAAGGTCTCAGAAGACAAAAAAGCAAAATGTGCAGAAAAG
GGGAAATCAAAACAGACAGAGTGCCTCAACTACATCCGGGTGCTGCAGCCACTCAGCGCC
ACTTCCCTTTACGTGTGTGGGACCAACGCATTCCAGCCGGCCTGTGACCACCTGAACTTA
ACATCCTTTAAGTTTCTGGGGAAAAATGAAGATGGCAAAGGAAGATGTCCCTTTGACCCA
GCACACAGCTACACATCCGTCATGGTTGATGGAGAACTTTATTCGGGGACGTCGTATAAT
TTTTTGGGAAGTGAACCCATCATCTCCCGAAATTCTTCCCACAGTCCTCTGAGGACAGAA
TATGCAATCCCTTGGCTGAACGAGCCTAGTTTCGTGTTTGCTGACGTGATCCGAAAAAGC
CCAGACAGCCCCGACGGCGAGGATGACAGGGTCTACTTCTTCTTCACGGAGGTGTCTGTG
GAGTATGAGTTTGTGTTCAGGGTGCTGATCCCACGGATAGCAAGAGTGTGCAAGGGGGAC
CAGGGCGGCCTGAGGACCTTGCAGAAGAAATGGACCTCCTTCCTGAAAGCCCGACTCATC
TGCTCCCGGCCAGACAGCGGCTTGGTCTTCAATGTGCTGCGGGATGTCTTCGTGCTCAGG
TCCCCGGGCCTGAAGGTGCCTGTGTTCTATGCACTCTTCACCCCACAGCTGAACAACGTG
GGGCTGTCGGCAGTGTGCGCCTACAACCTGTCCACAGCCGAGGAGGTCTTCTCCCACGGG
AAGTACATGCAGAGCACCACAGTGGAGCAGTCCCACACCAAGTGGGTGCGCTATAATGGC
CCGGTACCCAAGCCGCGGCCTGGAGCGTGCATCGACAGCGAGGCACGGGCCGCCAACTAC
ACCAGCTCCTTGAATTTGCCAGACAAGACGCTGCAGTTCGTTAAAGACCACCCTTTGATG
GATGACTCGGTAACCCCAATAGACAACAGGCCCAGGTTAATCAAGAAAGATGTGAACTAC
ACCCAGATCGTGGTGGACCGGACCCAGGCCCTGGATGGGACTGTCTATGATGTCATGTTT
GTCAGCACAGACCGGGGAGCTCTGCACAAAGCCATCAGCCTCGAGCACGCTGTTCACATC
ATCGAGGAGACCCAGCTCTTCCAGGACTTTGAGCCAGTCCAGACCCTGCTGCTGTCTTCA
AAGAAGGGCAACAGGTTTGTCTATGCTGGCTCTAACTCGGGCGTGGTCCAGGCCCCGCTG
GCCTTCTGTGGGAAGCACGGCACCTGCGAGGACTGTGTGCTGGCGCGGGACCCCTACTGC
GCCTGGAGCCCGCCCACAGCGACCTGCGTGGCTCTGCACCAGACCGAGAGCCCCAGCAGG
GGTTTGATTCAGGAGATGAGCGGCGATGCTTCTGTGTGCCCGGATAAAAGTAAAGGAAGT
TACCGGCAGCATTTTTTCAAGCACGGTGGCACAGCGGAACTGAAATGCTCCCAAAAATCC
AACCTGGCCCGGGTCTTTTGGAAGTTCCAGAATGACGTGTTGAAGGCCGAGAGCCCCAAG
TACGGTCTTATGGGCAGAAAAAACTTGCTCATCTTCAACTTGTCAGAAGGAGACAGTGGG
GTGTACCAGTGCCTGTCAGAGGAGAGGGTTAAGAACAAAACGGTCTTCCAAGTGGTCGCC
AAGCACGTCCTGGAAGTGAAGGTGGTTCCAAAGCCCGTAGTGGCCCCCACCTTGTCAGTT
GTTCAGACAGAAGGTAGTAGGATTGCCACCAAAGTGTTGGTGGCATCCACCCAAGGGTCT
TCTCCCCCAACCCCAGCCGTGCAGGCCACCTCCTCCGGGGCCATCACCCTTCCTCCCAAG
CCTGCGCCCACCGGCACATCCTGCGAACCAAAGATCGTCATCAACACGGTCCCCCAGCTC
CACTCGGAGAAAACCATGTATCTTAAGTCCAGCGACAACCGCCTCCTCATGTCCCTCTTC
CTCTTCTTCTTTGTTCTCTTCCTCTGCCTCTTTTTCTACAACTGCTATAAGGGATACCTG
CCCAGACAGTGCTTGAAATTCCGCTCGGCCCTACTAATTGGGAAGAAGAAGCCCAAGTCA
GATTTCTGTGACCGTGAGCAGAGCCTGAAGGAGACGTTAGTAGAGCCAGGGAGCTTCTCC
CAGCAGAATGGGGAGCACCCCAAGCCAGCCCTGGACACCGGCTATGAGACCGAGCAAGAC
ACCATCACCAGCAAAGTCCCCACGGATAGGGAGGACTCACAGAGGATCGACGACCTTTCT
GCCAGGGACAAGCCCTTTGACGTCAAGTGTGAGCTGAAGTTCGCTGACTCAGACGCAGAT
GGAGAC

Protein sequence predicted based on nucleotide sequence

MRMCTPIRGLLMALAVMFGTAMAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKD
TLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSA
TSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYN
FLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSV
EYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNVLRDVFVLR
SPGLKVPVFYALFTPQLNNVGLSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNG
PVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNY
TQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSS
KKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALHQTESPSR
GLIQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNDVLKAESPK
YGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSV
VQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQL
HSEKTMYLKSSDNRLLMSLFLFFFVLFLCLFFYNCYKGYLPRQCLKFRSALLIGKKKPKS
DFCDREQSLKETLVEPGSFSQQNGEHPKPALDTGYETEQDTITSKVPTDREDSQRIDDLS
ARDKPFDVKCELKFADSDADGD

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  mammalian    Marker:  blasticidin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable