WS0071 (W. succinogenes DSM 1740 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  WS0071
Gene Name:  phosphodiesterase
Original Clone ID:
APC64334_SG212_pMCSG19
Keyword:
full length
Species:
Wolinella succinogenes DSM 1740
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jessica Bearden
PSI
Midwest Center for Structural Genomics
WsCD00449726
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1419nts
Open reading frame: 44 to 1462

AATGGGGGGATTGGGTACGAAACCTGTACTTCCATCCAATGCCATGATTCTCCAAGAGGC
AAAGATTCGCGCCAAAGAGCTAGAGATTGGCGCCAAAAGCAAATATGAAAACGAGACCTC
CAAGGTCATCAAGGAGTACGAGAGCTATCTCATCAAGCTAGAGAAACAAGAGGCAAAAAA
TCAACAACGCCTAGAGCGTGAAACGCAACAGCTTGAAGAGGAGCGGCGAAGAATCAATCA
CAAGCAAGCCTCTCTCTCTGGCATGATTGAAGAGCTAGAGAAGCTCAAGGGCGACTACAG
GTCAAAACTCACCGAATCGCTAGAGATTCTCTCTCGCGTTGCAGGCCTCACCCAGGAAGA
GGCTAAAGAGCTTGCCCTTGCCAAAGCCACCGAAGAATCAAGAGCGCAGATCGCGCAGAT
CGTACGAAAATATGAGAGCGAGGCCAAAGAAGAGGGGCGCAAGCGCGCCAACTATATTTT
GGCACAGGCAACCACCCGCTTTGCTGGAGAGTTTGCCGCTGAGCGCCTCATCAATGTGGT
GAATCTCCCCAATGATGAACTCAAAGGACGCATCATTGGGAAAGAGGGGCGCAACATCAA
GACCCTCGAAATGCTCTCAGGCGTGGATGTAATCATCGATGATACTCCTGGGGCGATCGT
GCTTAGCAGCTTCAACCTCTATCGGCGCGCCATCGCCACTCGCACCATTGAGGCGTTGGT
GGAAGATGGAAGAATCCAGCCTGCTCGAATCGAAGAGGTCTATAAAAAAGTAGAACAAGA
GTTTGAAGAGAAGGTCCTCGAAGAGGGTGAGGGTATTGTTTTGGATATGGAGCTTGGCTA
TGTTCACCCCGAACTCAAAAAGCTCATTGGCCGCCTCAAATATCGCGCCAGCTATGGGCA
AAATGCCCTAGGCCACTCCCTCGAAGTCGCTAACCTCGCTGGTGTGATCGCTGGAGAGCT
TGGAGGTGATGTGAAACTCGCCAAAAGGGCAGGTTTGTTGCATGACATTGGCAAGGCTCT
AACCCATGACTTTGGCGGAAGCCATGTCGACTTGGGCGCGGAGGTGTGCAGTCGCTACAA
AGAGCACCCCGTGGTGATCAACGCCATCTTTGCCCACCATGGTCACGAAGAGGCCAAGAG
CATCGAGAGTGCCGCTGTCTGCGCGGCTGATGCCCTCTCAGCGGCTAGACCTGGAGCTAG
ACGCGAGGTGCTAGAGAGCTTCCTTAAGCGTGTTCAAGAGATTGAAAAGATTGCGATGGA
GAAGCTGGGTGTGCGCCAAGCCTACGCGATTAATGCAGGAAGAGAGGTTCGAGTCATCGT
CAACGCCGACCTCATCAACGATGAAGAGAGCGTTTTGCTTGCCCAAGAGATCGCCAAAGA
GATTGAGACCAAAGTCCAATATCCAGGCGAAATCAAAGTGAGCGTGATTCGTGAGGTTCG
GGCAGTGGAGTTTGCGAAGTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGT

Protein sequence predicted based on nucleotide sequence

MILQEAKIRAKELEIGAKSKYENETSKVIKEYESYLIKLEKQEAKNQQRLERETQQLEEE
RRRINHKQASLSGMIEELEKLKGDYRSKLTESLEILSRVAGLTQEEAKELALAKATEESR
AQIAQIVRKYESEAKEEGRKRANYILAQATTRFAGEFAAERLINVVNLPNDELKGRIIGK
EGRNIKTLEMLSGVDVIIDDTPGAIVLSSFNLYRRAIATRTIEALVEDGRIQPARIEEVY
KKVEQEFEEKVLEEGEGIVLDMELGYVHPELKKLIGRLKYRASYGQNALGHSLEVANLAG
VIAGELGGDVKLAKRAGLLHDIGKALTHDFGGSHVDLGAEVCSRYKEHPVVINAIFAHHG
HEEAKSIESAAVCAADALSAARPGARREVLESFLKRVQEIEKIAMEKLGVRQAYAINAGR
EVRVIVNADLINDEESVLLAQEIAKEIETKVQYPGEIKVSVIREVRAVEFAK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC64334
Recommended expression in:
Not Applicable