degS (B. subtilis subsp. subtilis str. 168 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  degS
Gene Name:  signal transduction histidine-protein kinase/phosphatase DegS
Original Clone ID:
APC37674.2_SG230_pMCSG19
Keyword:
partial cds
Species:
Bacillus subtilis subsp. subtilis str. 168
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Kimberly Buck
PSI
Midwest Center for Structural Genomics
BsCD00449841
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1098nts
Open reading frame: 42 to 1139

TTCGGGGGATTGGGTACGAAACCTGTACTTCCATCCATGCCAAAACAAAGATGGATTCCA
AAGTGCTGGATTCTATTTTGATGAAGATGCTGAAAACCGTTGACGGGAGCAAGGACGAGG
TTTTTCAAATCGGGGAGCAGTCACGCCAGCAGTATGAACAGCTGGTCGAAGAACTGAAAC
AAATTAAACAGCAGGTGTATGAAGTGATTGAGCTTGGCGATAAACTTGAAGTGCAAACTC
GCCATGCGAGAAACCGTTTATCCGAGGTCAGCCGTAATTTTCATAGATTCAGTGAAGAGG
AAATCCGCAATGCTTATGAAAAAGCCCATAAGCTGCAGGTAGAATTGACGATGATCCAGC
AGCGTGAGAAGCAATTGCGCGAACGGCGGGACGATTTGGAGCGCAGATTGCTAGGGCTTC
AGGAAATCATTGAGCGGTCAGAATCATTAGTAAGCCAAATTACAGTTGTGCTCAACTACT
TGAATCAGGATTTGCGCGAAGTTGGACTGCTTCTTGCTGATGCTCAGGCAAAACAGGATT
TCGGCTTAAGAATTATTGAGGCGCAGGAAGAAGAGCGAAAAAGAGTCTCAAGAGAAATCC
ATGACGGACCCGCTCAAATGCTGGCGAATGTTATGATGAGATCGGAATTAATCGAGCGGA
TTTTCCGTGACCGGGGCGCAGAGGACGGATTCCAAGAAATTAAAAATCTCCGCCAAAATG
TTCGGAATGCCCTTTACGAAGTGAGAAGGATTATATATGATTTAAGACCGATGGCCCTTG
ATGACCTAGGCCTGATTCCAACTTTAAGAAAATATCTATATACAACCGAGGAATATAACG
GGAAGGTCAAAATACATTTTCAGTGCATTGGAGAAACAGAGGATCAGAGGCTAGCGCCTC
AGTTTGAGGTTGCGCTCTTCAGGCTCGCACAGGAAGCTGTGTCTAATGCGCTAAAGCATT
CTGAATCTGAAGAAATTACAGTCAAAGTTGAGATCACAAAGGATTTTGTGATTTTAATGA
TAAAAGATAACGGTAAAGGGTTCGACCTGAAGGAAGCGAAAGAGAAGAAAAACAAATCAT
TCGGCTTGCTGGGCATGAAAGAAAGAGTAGATTTATTGGAAGGAACGATGACAATATAAC
ATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTCGACA

Protein sequence predicted based on nucleotide sequence

KTKMDSKVLDSILMKMLKTVDGSKDEVFQIGEQSRQQYEQLVEELKQIKQQVYEVIELGD
KLEVQTRHARNRLSEVSRNFHRFSEEEIRNAYEKAHKLQVELTMIQQREKQLRERRDDLE
RRLLGLQEIIERSESLVSQITVVLNYLNQDLREVGLLLADAQAKQDFGLRIIEAQEEERK
RVSREIHDGPAQMLANVMMRSELIERIFRDRGAEDGFQEIKNLRQNVRNALYEVRRIIYD
LRPMALDDLGLIPTLRKYLYTTEEYNGKVKIHFQCIGETEDQRLAPQFEVALFRLAQEAV
SNALKHSESEEITVKVEITKDFVILMIKDNGKGFDLKEAKEKKNKSFGLLGMKERVDLLE
GTMTI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Applicable
SolubleProtein: Protein_Soluble
ProteinExpressed: Tested_Not_Found
Find experimental protocols in APC37674.2
Recommended expression in:
Not Applicable