VCA0140 (V. cholerae O1 biovar El Tor str. N16961 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  VCA0140
Gene Name:  spindolin-like protein
Original Clone ID:
APC64147_SG187_pMCSG19
Keyword:
full length
Species:
Vibrio cholerae O1 biovar El Tor str. N16961
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Kimberly Buck
PSI
Midwest Center for Structural Genomics
VcCD00449888
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1209nts
Open reading frame: 47 to 1255

AAAATCGGCGGGATTGGGTACGAAACCTGTACTTCCATCCAATGCCATGGCGAATAACAA
GAGTCACAATCAAGAAGGAATAACCATGAAATACGGATTAAAAATCAGTGCGCTAGGCTC
AGTCATACTCGGGGTTTTGGCGGCTTCGCAGGTACAAGCACATGGTTGGGTGGAATTCCC
AAGCGCAAGACAAAACACCTGTTACCTCGACGGCGGTTTTTGGGATAACGCGATTCCAAA
TCAGGCTTGTCAGGCGGCCTATGATGTTTCCGGTGCTTTTCCGTTTGTGCAGCGCAATGA
AATATCGGCCAATGTGCAGAAGTATCGGGATATGGCCGCAGTAAAAGCGGTGGTGAAAGA
TGGCGAGCTCTGTTCGGCGGGCGATAAAGCCAAAGCGGGCTTGAATGTGCCTTCTGCCCA
TTGGCAAAAAACCGGAATCACCCTAGATGCCAATGGTCAAATTGAGGTCGTGTTCCATGC
GGCAACCCCTCACAATCCCTCGTATTGGCAATTTTATTTAAGTAAAGCGACTTACGACCA
CACCAAGCCACTGACGTGGGATGACTTGGAATTGGTTGGCAGCTCGGACGATGTGGCCGC
GGGAAGTGATAAGAAATACCGTTTTAAAGTCACCTTGCCACAAGATCGCAGTGGCGATGC
GATTCTTTACACGCGCTGGCAGCGGGTAGATGCAGGTGGTGAAGGCTTTTATAACTGCAG
TGACATCACCTTTGGCGGCACTACCACGCCACCAGATCCGACTGACCCGACCGATCCGGT
TAAGCTCACCGCTTTGGGTTACTACGTACCACAAGGGTTTGGCCCTGTTGAAGTCGGAGA
CAGTGTTCGCTTGCGAACATTTGATACGACAGGTATGGAAACGACAGATATTGCCTTGCC
AATCCGTGCCAACAATTTGGAGACTTGGCCTGCCGAGCTCGCGGGTCAGTTTAATCAACT
GAAAAACGGCGAATGGTTTATTGGTATTTGGCACCAAGAGATGAACCATTACATGTTTGA
TACGCAAAATCTGTACGCCAACCAAGTCTTTGCGCCAAACAGTAACCTCACTTACCGCTT
ATCGCTGACGAAGGGAGATACCACGCCACCGACTCAACCCGAAAATGCATGGGATAAGAG
CAAGACTTATACCGCGGGTGCGGTAGTCACTCATAAAGGCAAGAGCTGGACTGCCCAGTG
GTGGACACAAGGTGAAGAGCCGGGAACCACAGGCGAATGGGGTGTGTGGCGCTAACATTG
GAAGTGGATAACGGATCCGAATTCGAGCTCC

Protein sequence predicted based on nucleotide sequence

MANNKSHNQEGITMKYGLKISALGSVILGVLAASQVQAHGWVEFPSARQNTCYLDGGFWD
NAIPNQACQAAYDVSGAFPFVQRNEISANVQKYRDMAAVKAVVKDGELCSAGDKAKAGLN
VPSAHWQKTGITLDANGQIEVVFHAATPHNPSYWQFYLSKATYDHTKPLTWDDLELVGSS
DDVAAGSDKKYRFKVTLPQDRSGDAILYTRWQRVDAGGEGFYNCSDITFGGTTTPPDPTD
PTDPVKLTALGYYVPQGFGPVEVGDSVRLRTFDTTGMETTDIALPIRANNLETWPAELAG
QFNQLKNGEWFIGIWHQEMNHYMFDTQNLYANQVFAPNSNLTYRLSLTKGDTTPPTQPEN
AWDKSKTYTAGAVVTHKGKSWTAQWWTQGEEPGTTGEWGVWR*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Applicable
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC64147
Recommended expression in:
Not Applicable