Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
APC1527.3_SG228_pMCSG19
Keyword:
partial cds
Species:
Bacillus subtilis subsp. subtilis str. 168
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Kimberly Buck
PSI
Midwest Center for Structural Genomics
BsCD00449954
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1065nts
Open reading frame: 16 to 1080

CTTCCATCCAATGCCTCAGCTTATATCCATATCCCATTTTGTGAGCATATTTGCCACTAC
TGCGATTTCAATAAATATTTTATTCAAAGTCAGCCAGTCGACGAGTATTTAAACGCACTC
GAACAGGAAATGATAAATACAATAGCAAAAACCGGGCAGCCTGATCTCAAAACAATCTTT
ATCGGAGGAGGCACGCCGACCTCCCTGTCTGAGGAACAGCTTAAAAAGCTGATGGATATG
ATCAACCGCGTGCTAAAGCCCTCAAGCGACTTATCGGAATTTGCGGTTGAAGCGAATCCG
GACGATTTGTCCGCAGAGAAATTAAAAATATTAAAAGAAGCAGGCGTAAATCGCCTTAGC
TTTGGTGTGCAAACGTTCGAAGACGATCTGTTAGAAAAAATCGGCCGAGTGCACAAGCAA
AAAGATGTATTCACCTCTTTTGAAAGAGCGCGGGAAATCGGTTTTGAGAATATCAGTCTT
GATCTCATGTTCGGGCTTCCGGGGCAAACCCTCAAGCATCTGGAACATTCCATAAACACT
GCTTTATCATTGGACGCTGAGCATTATTCCGTTTACTCCCTTATTGTAGAACCGAAAACG
GTATTTTATAATTTGATGCAAAAAGGGCGGCTTCATCTTCCGCCGCAGGAGCAGGAAGCT
GAAATGTATGAAATAGTGATGAGCAAGATGGAAGCTCACGGCATTCATCAATATGAAATC
AGCAACTTTGCTAAGGCAGGCATGGAATCTAAGCACAATCTAACGTATTGGAGCAACGAA
CAATATTTCGGTTTCGGAGCTGGCGCTCACGGATATATTGGCGGCACACGAACAGTGAAC
GTCGGACCTGTTAAGCATTACATTGACCTGATAGCTGAAAAGGGTTTTCCGTACAGAGAT
ACACATGAAGTGACAACAGAAGAACAGATTGAGGAAGAAATGTTTTTAGGTTTGAGAAAA
ACAGCCGGTGTCAGCAAAAAACGATTCGCAGAAAAATACGGCCGTTCCCTGGATGGACTT
TTCCCAAGCGTGTTAAAAGACCTCGCTGAAAAAGGGCTGATTCACAATTCGGAGTCTTAA
CATTGGAAGTGGATAACGGAT

Protein sequence predicted based on nucleotide sequence

SAYIHIPFCEHICHYCDFNKYFIQSQPVDEYLNALEQEMINTIAKTGQPDLKTIFIGGGT
PTSLSEEQLKKLMDMINRVLKPSSDLSEFAVEANPDDLSAEKLKILKEAGVNRLSFGVQT
FEDDLLEKIGRVHKQKDVFTSFERAREIGFENISLDLMFGLPGQTLKHLEHSINTALSLD
AEHYSVYSLIVEPKTVFYNLMQKGRLHLPPQEQEAEMYEIVMSKMEAHGIHQYEISNFAK
AGMESKHNLTYWSNEQYFGFGAGAHGYIGGTRTVNVGPVKHYIDLIAEKGFPYRDTHEVT
TEEQIEEEMFLGLRKTAGVSKKRFAEKYGRSLDGLFPSVLKDLAEKGLIHNSES*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c276a,D92E
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Applicable
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in APC1527.3
Recommended expression in:
Not Applicable