PA5546 (P. aeruginosa PAO1 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PA5546
Gene Name:  hypothetical protein
Original Clone ID:
APC37523.1_SG226_pMCSG19
Keyword:
partial cds
Species:
Pseudomonas aeruginosa PAO1
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Kimberly Buck
PSI
Midwest Center for Structural Genomics
PaCD00449978
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1140nts
Open reading frame: 46 to 1185

AAATTTGGGGAATTGGGTACGAAACCTGTACTTCCATCCAATGCCATGATTGCACAGCTC
CCCTCTGAGTTGCTGTCCCTGCAACTGCCGTTGCGGATCCGCATCGGCGATGCTCAGGCG
TTCGATCTGGGGCCGGATCCACAGGTAACCATGGTCATTCGCGACCCCTCGCTGCTAACC
GAGATCACCCATCCCAGCCTCGATGCGCTGGGCAAGGCCTACGTCGAGAAGCGTCTCGAC
ATCGAAGGACCGATCAGCCAGGTCATCGGTATCGGCGATGCCCTCAGCGAAGCGCTCGGC
GAACCCGATGGCGGCGCGCCGCCGGTTCGCGAAAGCCACGACAAGGACACAGACGCCGAA
GCCATCCATTACCACTACGACCTGTCCAACGACTTCTACCAGCTCTGGCTGGATCCGGAA
ATGGTCTATTCCTGCGCTTATTTCGAGACCGGCAAGGAAGACCTCGCCACCGCGCAACTG
GCCAAGCTCCGTCACCTGTGCCGCAAGCTGCGTTTGCAGCCGGGCGAGAAGCTGCTCGAT
GTCGGTTGCGGTTGGGGAGGGCTGGCGCGCCTGGCCGCTCGCGAGTTCGGCGTCGAGGTC
ACCGGCATCACATTGAGCAAGGAGCAGCTGGCGCTCGGCCGGGAGCGAGTCAAGGCCGAA
GGCCTGGAGGACCGAGTCACCCTCAAGCTGCTGGATTACCGCGACCTGCCGCGCGATGGC
CGCTTCGACAAGGTGGTCAGCGTCGGCATGTTCGAGCACGTCGGGCACGCCAACCTGGGG
CTGTACTTCCAGCAACTGTACGAGGCGGTGCGTCCCGGCGGCCTGGTGATGAACCACGGC
ATCACCTCGCGCTACACCGACGGCCGCCCGGTTGGCAAGGGGGCCGGCGACTTCATCGAT
CGCTACGTGTTTCCCCACGGCGAGCTGCCGCACCTGTCGCTGGCGGTGGCGCGGATGAGC
GAAGCCGGGCTGGAAGTAGTCGACGTGGAAAGCCTGCGTCTGCACTACGCCCGGACCCTG
GACTTCTGGAGCGCCAACCTGGAAGCCAGGCTGAAGGAGGCGGCTCGCCTGGTGCCCGAG
GAAACCTTGCGGATCTGGCGGCTGTACCTGGCCGGATGCGCCTACGGCTTCAAGCGCGGC
TGGATCAACCTGCACCAGATCCTCGCCATTCGCCCCCACGAGTAACATTGGAAGTGGATA
ACGGTAA

Protein sequence predicted based on nucleotide sequence

MIAQLPSELLSLQLPLRIRIGDAQAFDLGPDPQVTMVIRDPSLLTEITHPSLDALGKAYV
EKRLDIEGPISQVIGIGDALSEALGEPDGGAPPVRESHDKDTDAEAIHYHYDLSNDFYQL
WLDPEMVYSCAYFETGKEDLATAQLAKLRHLCRKLRLQPGEKLLDVGCGWGGLARLAARE
FGVEVTGITLSKEQLALGRERVKAEGLEDRVTLKLLDYRDLPRDGRFDKVVSVGMFEHVG
HANLGLYFQQLYEAVRPGGLVMNHGITSRYTDGRPVGKGAGDFIDRYVFPHGELPHLSLA
VARMSEAGLEVVDVESLRLHYARTLDFWSANLEARLKEAARLVPEETLRIWRLYLAGCAY
GFKRGWINLHQILAIRPHE*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC37523.1
Recommended expression in:
Not Applicable