ypgR (B. subtilis subsp. subtilis str. 168 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  ypgR
Gene Name:  hypothetical protein
Original Clone ID:
APC1447_SG199_pMCSG19
Keyword:
full length
Species:
Bacillus subtilis subsp. subtilis str. 168
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jessica Bearden
PSI
Midwest Center for Structural Genomics
BsCD00450058
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1134nts
Open reading frame: 3 to 1136

CCATGAAGATAAAATCAATTGAACCTACACCAAGCCCGAATACAATGAAAGTCATCTTAA
CGGAAGAGCTTCCGGCGGGCAAAAGCAATAACTATAAACCTGAGCAAGCAGAAGGCGCAC
CGCTTGTTATAGCGGAAATCCTTAAAATAGATGGAGTTAAAGGGGTATATCACGTCGCAG
ATTTTCTTGCTGTTGAACGGAATGCAAGATACGACTGGAAAGACATTTTACCGCAAGTCC
GCACTGCATTTGGGATGGAAAGTGCTGAAAGCGCAGAAGAAAATCGTTCTGATCAAGAAT
CTTTTGGAGAGGTAAAAGTTTTTGTGCAGATGTTCAGCGGCATTCCTATGCAAGTTAAAT
TAACCGATGGAGAGCGGGAAGAACGCTTCGGCTTACCTGAACGCTTTCAGCAGGCGATCT
TAAAACTTAGATCTGAAGCTTCCAATGTTGTGTTTGAGCGGGCTTGGAAAGAACAAGGTG
TCAGATTCGGTGACTTTGATGAAATCGGCCATGATGTGACAGAAGAGCTTCAAGCGGCAT
ACAGTGATGAACGGCTGAAACGACTGACTGAAGCAGCCGCTCAAGGAAAGGGCGAAGCAA
AACAAGCTGTACAGCGCAAAGCGTATAAAGTAACGCTTGATATGCTGGATGATGAGGACT
GGAAAAAGCGTTATGCACACTTGGAACAGATGGATCCGAAAGAAGAGGATATCCCGGTTT
TACAGAAGGCATTGGATGATCCAAAGGTGTCCATCAGAAGACAGGCTGTTGTGTACTTAG
GAATGATTGAAACACCTGATGTTCTTCCTCTATTGTATAAAGCACTTGAGGACAAAGCTG
TATCAGTCAGAAGAACGGCCGGAGACTGCCTGTCTGATATCGGCGATCCTCAAGCCATTC
CTGCTATGATCAAGTCATTAAGCGACTCCAGCAAGCTTGTTCGCTGGCGTGCCGCCATGT
TCCTGTACGAAGTCGGCGATGAAAGTGCAATTGAAGCTTTGCGCGCTGCCGAAGATGACC
CCGAATTTGAGGTCAGCCTTCAAGTCAAAATGGCGCTTGAACGTATTGAGCATGGAGAAG
AAGCAAAAGGTTCTGTTTGGAAACAAATGACGGAAAGCAGAAAAAAAGGCGAATAACATT
GGAAGTGGATAACGGTAA

Protein sequence predicted based on nucleotide sequence

MKIKSIEPTPSPNTMKVILTEELPAGKSNNYKPEQAEGAPLVIAEILKIDGVKGVYHVAD
FLAVERNARYDWKDILPQVRTAFGMESAESAEENRSDQESFGEVKVFVQMFSGIPMQVKL
TDGEREERFGLPERFQQAILKLRSEASNVVFERAWKEQGVRFGDFDEIGHDVTEELQAAY
SDERLKRLTEAAAQGKGEAKQAVQRKAYKVTLDMLDDEDWKKRYAHLEQMDPKEEDIPVL
QKALDDPKVSIRRQAVVYLGMIETPDVLPLLYKALEDKAVSVRRTAGDCLSDIGDPQAIP
AMIKSLSDSSKLVRWRAAMFLYEVGDESAIEALRAAEDDPEFEVSLQVKMALERIEHGEE
AKGSVWKQMTESRKKGE*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC1447
Recommended expression in:
Not Applicable