lipA (P. gingivalis W83 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  lipA
Gene Name:  lipoyl synthase
Original Clone ID:
APC80816.1_SG166_pMCSG19
Keyword:
partial cds
Species:
Porphyromonas gingivalis W83
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
PhCD00450151
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 801nts
Open reading frame: 45 to 845

TTCTCGGGGGATTGGGTACGAAACCTGTACTTCCATCCAATGCCGGTGGGAATGAGAAGT
TTACCGAGACTAAAAGCATTGTCGAGGGACATTGTCTGCATACGATCTGTACAAGTGGCA
AATGCCCCAATATGGGAGAGTGCTGGAGTAGGGGGACTGCTACCTTCATGATCGGTGGTG
ATATCTGTACACGTGCCTGTCGATTTTGCAACACTTTGACGGGGCGTCCCAAACCGCTCA
ATGAAGCAGAACCTACTCACGTCGCATTGAGTATCAAACTGATGGGGCTTAACCATGCTG
TGGTGACGAGCGTTGACCGCGATGACTTGCCGGATTATGGTGCCGCACACTGGGTTAAGA
CCATTCAGGAAATCAGACGGATCAACTCGGGAGTGACACTCGAAGTTCTGATTCCCGACT
TCAAAGGACGGATGGATTTGGTGGACATGATTATCGAGGCAAGTCCGGATGTTATTTCGC
ATAATCTGGAGACGGTACGTCGGCTTACGCCATCGGTACGCAGCGTGGCTACTTACGATA
CCAGCCTTGCCGTGCTTCGTCACATAGCTCAGTCCGGAAAAATGCCCGCGAAAACAGGAA
TGATGCTCGGTTTGGGCGAGACTGAAGAAGAGATACTCGAATTGATGGATGATGCTCTTG
CGGCGGGAGTCTCAGTTATTACTATAGGACAGTATCTGCAACCGTCGAGGAAGAATCTTC
CTGTGGTGGAATACATCACGCCCGAACAGTTCGAACATCTTCGACTGGTGGGTATCGAAA
AAGGCTTCCGTACGATAGAGAGTGCTCCGCTCGTTCGCAGTTCTTACCATGCCGAACGGC
ACTAACATTGGAAGTGGATAACGGTAA

Protein sequence predicted based on nucleotide sequence

GGNEKFTETKSIVEGHCLHTICTSGKCPNMGECWSRGTATFMIGGDICTRACRFCNTLTG
RPKPLNEAEPTHVALSIKLMGLNHAVVTSVDRDDLPDYGAAHWVKTIQEIRRINSGVTLE
VLIPDFKGRMDLVDMIIEASPDVISHNLETVRRLTPSVRSVATYDTSLAVLRHIAQSGKM
PAKTGMMLGLGETEEEILELMDDALAAGVSVITIGQYLQPSRKNLPVVEYITPEQFEHLR
LVGIEKGFRTIESAPLVRSSYHAERH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in APC80816.1
Recommended expression in:
Not Applicable