CHU_3263 (C. hutchinsonii ATCC 33406 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CHU_3263
Gene Name:  radical SAM protein
Original Clone ID:
APC62817_SG175_pMCSG19
Keyword:
full length
Species:
Cytophaga hutchinsonii ATCC 33406
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
ChCD00450220
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1020nts
Open reading frame: 46 to 1065

ACCTCGGCGGGATTGGGTACGAAACCTGTACTTCCATCCAATGCCATGATTGCTACCGGT
GATGCACTGAATTTACTTTCTAAAATGACCTTCAAAAGGCTTATAACGCTTTTGAAGGTA
TTTGTCAGTTATCAGTATTCGAAGATCACCGGCAATGTAGTACATCCTTCTGTGCCGTAC
TCCATTGCAATAGAGCCAACAACATCCTGTAATCTGCGCTGCCCCGAATGCCCGAGCGGG
TTGCGCTCTTTTACACGGCCAACAGGCATGCTGAACATTGCCATGTTCTCTAAAATTATT
GATGAATTATATCAGCAGATCAGTTACCTGATTTTTTATTTTCAGGGAGAACCTTATTTG
AATACCTCTTTTTTAGAAATGGTTAAATATGCGTCTTCCAAAGGAATTTATACGGCTACT
TCTACAAACGCCCATTATCTCACAGACGAAAATGCACGCAAGACCGTAGAATCAGGACTT
GACCGGTTGATCATTTCGATTGATGGCACTACACAGGAAGTCTACGAACAATACCGCATC
GGGGGAAGCTTAGAAAAAGTTCTGGAAGGAACTAAAAATATTCTTGCCTGGAAGAAAAAA
CTACACGCTTCTACACCGCATGTAATTTTTCAATTTTTAGTGGTGAAACCCAATCAACAT
CAATTGAAGGAAGTGAAAAAAATGGCAAAGGAATTGGGTGTTGGTGAAGTGCGTTTTAAA
ACAGCGCAGATCCATGATTATGAAAACGGTTCGCCATTGATTCCCGATATTGATAAATAC
GCCCGCTACAGAAAAAATGCAGATAATACTTATTCCATAAAAAACAGTTTGTTAAATCAT
TGCTGGAAGATGTGGCACTCCTGTGTGATTACCTGGGATGGTAAAATTGTACCGTGCTGT
TTTGATAAGGATGCGCACCTTGTAATGGGTGAGCTGAAAACAAATACGTTTAAGGAAATC
TGGAACAGCGCACCGTATATTCAATTCAGGAAATCACTTTTGAAATCGAGAAGTGAAATT
GATATGTGTAAGAATTGTACCGAAGGAACTACAGTATGGGCATAACATTGGAAGTGGATA
ACGGTAA

Protein sequence predicted based on nucleotide sequence

MIATGDALNLLSKMTFKRLITLLKVFVSYQYSKITGNVVHPSVPYSIAIEPTTSCNLRCP
ECPSGLRSFTRPTGMLNIAMFSKIIDELYQQISYLIFYFQGEPYLNTSFLEMVKYASSKG
IYTATSTNAHYLTDENARKTVESGLDRLIISIDGTTQEVYEQYRIGGSLEKVLEGTKNIL
AWKKKLHASTPHVIFQFLVVKPNQHQLKEVKKMAKELGVGEVRFKTAQIHDYENGSPLIP
DIDKYARYRKNADNTYSIKNSLLNHCWKMWHSCVITWDGKIVPCCFDKDAHLVMGELKTN
TFKEIWNSAPYIQFRKSLLKSRSEIDMCKNCTEGTTVWA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC62817
Recommended expression in:
Not Applicable