thiI (A. fischeri ES114 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  thiI
Gene Name:  thiamine biosynthesis protein ThiI
Original Clone ID:
APC64237_SG181_pMCSG19
Keyword:
full length
Species:
Aliivibrio fischeri ES114
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
VfCD00450392
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1449nts
Open reading frame: 45 to 1493

ACCTCGGGGGATTGGGTACGAAACCTGTACTTCCATCCAATGCCATGAAGTTTATCGTTA
AGCCACACCCAGAAGTTTTTGTTAAAAGTGATTCAGTACGTAAGCGCTTTATTCGTATTC
TAGAATCTAACCTACGCAGTATTATTCAGCGTGAAACCAAAGGTGTTGAAGTCATCAACC
GTCGTGATCATATTGAAGTGACAGGACTGAGTGATGAATACCGTGATGTAACATTGGCGG
TACTAACGCAAACTCCAGGTATTCATCATGTACTTGAAGTTAAGCAGTCAGGCTTTAAAG
ACATGCACGATATTTTTGAGCAATGTCTTGAAATGAACCGTGAAGTGATTGAAGGTAAAA
CGTTCTGTGTACGTGTTAAGCGTCGTGGTAATCATGACTTTACGTCTATTGAGCTTGAGC
GCTACGTTGGTGGCGGTTTAAATCAATCAGTAGAAAGTGCAAGTGTTCGTCTAAAGAACC
CTGAAGTGACGGTTAAATTTGAAGTAGCAAATGATAAATTAAACTTGATCATTGCTCGTC
ATAAAGGCTTGGGTGGTTTTCCTCTTGGAACTCAAGAAGATGTATTAAGCTTGATTTCTG
GTGGTTTTGATTCTGGTGTATCAAGCTACTTACACATTAAACGTGGTTCAAAAGTACACT
ACTTATTCTTTAATTTAGGTGGTCCAGCTCACGAGATTGGCGTGAAACAAGTGTCGCACT
TCTTGTGGAAAAAATACGGTTCATCAGCAAAAGTTAAATTTATCTCTGTTGATTTTGATC
CAGTTGTTGCTGAGATCTTAGAGAAAGTAGATGATGGCCAAATGGGTGTTATCTTAAAAC
GTATGTTTATGCGTGCAGGTGGTATGGTTGCTGAGAAATTTGGCATTGAAGGATTAGTTA
CTGGTGAAGCATTAGGTCAAGTATCTAGTCAAACACTAACTAACTTACGCCATATTGATA
ATGTAACTGATTCACTGATTCTACGTCCTCTAATTAACTGGGACAAAGAAGATATTATTG
ATTTAGCTCGTGAGATTGGCACTGAAGATTTCGCTAAAACAATGCCTGAATACTGTGGTG
TAATTTCTAAGAAACCAACAGTAAAAGCAGTAAAAGAAAAACTAGAAAAAGAAGAAGCGA
AATTCGATTTCTCTGTTCTTGAGCAAGCGGTTTATAACGCGCGAGTTATGGACATTCGTG
ATATTGAAAAAGAAAGCCAAGAGCAAGCTCCAGAAGTTGAAATGGTATCTGAGCTAGGTT
CTGATGTTGTGGTTCTTGATATCCGTAGTGCTGAAGAAGAAGATGAGAAGCCATTAGAGC
TTGATGGTGTTGAGGTGACTCACATTCCTTTCTTCAAATTAGCAACTAAGTTTGGTGATC
TAGACCAATCTAAAGAGTACTTATTATACTGTGAGCGTGGTGTGATGAGCCGTCTTCAAG
CATTATTATTAATTGAGAATGGCTATAAGAACGTAAAAGTTTACCGTCCATAACATTGGA
AGTGGATAACGGATCCGAATTCGAGCTCCGTCGACA

Protein sequence predicted based on nucleotide sequence

MKFIVKPHPEVFVKSDSVRKRFIRILESNLRSIIQRETKGVEVINRRDHIEVTGLSDEYR
DVTLAVLTQTPGIHHVLEVKQSGFKDMHDIFEQCLEMNREVIEGKTFCVRVKRRGNHDFT
SIELERYVGGGLNQSVESASVRLKNPEVTVKFEVANDKLNLIIARHKGLGGFPLGTQEDV
LSLISGGFDSGVSSYLHIKRGSKVHYLFFNLGGPAHEIGVKQVSHFLWKKYGSSAKVKFI
SVDFDPVVAEILEKVDDGQMGVILKRMFMRAGGMVAEKFGIEGLVTGEALGQVSSQTLTN
LRHIDNVTDSLILRPLINWDKEDIIDLAREIGTEDFAKTMPEYCGVISKKPTVKAVKEKL
EKEEAKFDFSVLEQAVYNARVMDIRDIEKESQEQAPEVEMVSELGSDVVVLDIRSAEEED
EKPLELDGVEVTHIPFFKLATKFGDLDQSKEYLLYCERGVMSRLQALLLIENGYKNVKVY
RP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t78a,L26L;c150t,I50I;
c765a,V255V;t774c,G258G;
c819t,G273G
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in APC64237
Recommended expression in:
Not Applicable