thiH (P. gingivalis ATCC 33277 )
In pMCSG19B (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  thiH
Gene Name:  thiamine biosynthesis protein ThiH
Original Clone ID:
APC66773.2_SG287_pMCSG19B
Keyword:
partial cds
Species:
Porphyromonas gingivalis ATCC 33277
Type:
cDNA
Vector Name:
pMCSG19B
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
PgCD00450427
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 984nts
Open reading frame: 18 to 1001

TACTTCCATCCAATGCCGACTTTATCACGCTTATTTCGCCTGCAGCCATCCCTTATCTGG
AGCAGATGGCCCGGCAGGCGCATGCCCTGACGGTAGAGCGATTCGGCCACACGATGCAGC
TCTATATCCCCCTCTACCTGTCGAATATCTGTAGCAATGCCTGTGTATACTGCGGATTCA
GTCGGTCGAACAAAATCCATCGCCGCCGACTCACTGCCGAAGAGGTGGATCGGGAAGCGG
AAGCGATCCTTCGGCTCGGATACAAACACCTCCTTCTCGTGTCGGGAGAGTCCGAGAAAG
CCACTCCGGCAAGCTACTATGAGAAAATGACGCGGCGACTGCGTCCACTCTTCTCCCAAC
TCTCGCTCGAAGTTCAGCCCCTGACCACGGAAGAATATGCCCGTCTGCATGAAGCCGGTA
TCGGAGCCGTCTATGTGTATCAGGAGACATACAACGAGCAGGCATACCCCACCTATCACC
CTGCCGGCCGGAAGGCGGACTATCGCTACCGCTTGGAGACTCCCGACAGAATCGGCCGAG
CCAATATGCAAAAGATCGGAATAGGGGCACTGCTGGGACTGGAGAATTGGCGTGTGGACT
CCGTTTTCACTGCTTTGCACCTGCGGTATCTGGAACAGACGTATTGGAAGAGCAAGTTTT
CCATCTCGCTGCCTCGTCTGCGTCCTGCCACGGGCGGCTGGGAGCCTAAAGATCCTATTG
ACGATGTCGGTATGGTACAGCTTATTACTGCCTTCCGTCTGTTGGATAAGGATGTCGAGA
TCAGCCTGTCCACACGGGAGAGTCGTGAGTTTCGTGACCACGTGATGCCGCTCGGTATCA
CCTCGGTCAGTGCCGGCAGCAAGACCGAACCCGGAGGATATGCCGAAGAGAATGCCGATC
TGGAGCAATTCGCCATCAACGATGCCCGCAGTCCGGCCGAAATGGCTGCCGATCTTCGCC
GACTTGGCTACGAGCCGGTTTGGAAAGACTGGGATGCTTAACATTGGAAGTGGATAACGG
ATCCGAATTCGAGCTCCGTCGACAAGCTTGCG

Protein sequence predicted based on nucleotide sequence

DFITLISPAAIPYLEQMARQAHALTVERFGHTMQLYIPLYLSNICSNACVYCGFSRSNKI
HRRRLTAEEVDREAEAILRLGYKHLLLVSGESEKATPASYYEKMTRRLRPLFSQLSLEVQ
PLTTEEYARLHEAGIGAVYVYQETYNEQAYPTYHPAGRKADYRYRLETPDRIGRANMQKI
GIGALLGLENWRVDSVFTALHLRYLEQTYWKSKFSISLPRLRPATGGWEPKDPIDDVGMV
QLITAFRLLDKDVEISLSTRESREFRDHVMPLGITSVSAGSKTEPGGYAEENADLEQFAI
NDARSPAEMAADLRRLGYEPVWKDWDA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g307a,E103K;a681c,G227G;
t697c,S233P;c702t,I234I;
t705c,D235D;a798t,R266R;
a876t,K292N;g879c,A293A;
a907g,t909c,T303A
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in APC66773.2
Recommended expression in:
Not Applicable