PH1867 (P. horikoshii OT3 )
In pMCSG19B (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PH1867
Gene Name:  hypothetical protein
Original Clone ID:
APC66586.1_SG292_pMCSG19B
Keyword:
partial cds
Species:
Pyrococcus horikoshii OT3
Type:
cDNA
Vector Name:
pMCSG19B
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
PhCD00450436
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 906nts
Open reading frame: 18 to 923

GTACTTCCATCCATGCCGGCCCAGGCGTTGGTTCTCCACAGAGCTACACCGGAAAGGAAC
CCTCAGCGTTAAGGGCTTCCCAGTATGGTTACCATCCCTATATAATCATGATGGCCAGAC
TTAAGCAATTATACGACATAGGTCATCCAATAGATAAGGTTGAGGTTATAATCCAGGGAG
GAACTTTTCCTGCGGTGGACTTGGATTATCAGGAGTGGTTCGTTAAGTGTGCATTCAAGG
CCATGAACGACTTTCACTACTTTAAGGATATTGATAACTTGGAGGAAAAGCTCGTTAGAC
TGATATTAAAGGGTGATAAATCAGTTTTCAAAGAGGATCCTGAATTTAAAAGGGCCTGGG
AGAGAACCCACAGGAAGCCTTATTACTACCTTGAAGATGAGCAAAGGAAAAATGAGAAAG
CTAAAGTTAGGATGGTTGGATTAACAATAGAGACGAGACCGGATTGGGCCTTTGAAAGAC
AAATTGATAGGATGTTAAGCTTTGGAACAACAAGAGTGGAACTTGGGGTTCAAACAATAT
TCAACTTTATACACGAGAGAACCAAGCGTGGCCATGGAGTTGAGGAAATTATTAAGGCTA
CCCAGTTATTAAAAGACGCTGGACTTAAGGTGAATTATCATATAATGCCTGGCCTCCCTG
GGAGCAACTTCGATAGGGATCTTTATACCTTTAGAACGATATTTGAAGATCCTAGGTTTA
GGCCTGACATGCTTAAAATATACCCCACCCTAGTCACCAGGGATGCTCCCCTTTATGCCT
GGTACAAGGCGGGTCTTTATAGGCCATACACAACTGAAGAAGCTCTTGAGCTTCTAGTTG
AGGCTTATAAGATTTTTCCAAAGTGGGTTAGGGTTATGAGAATTCAAAGGGATATACCTG
CTCACCTAATAGTCGCTGGATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCG
TCGACAAGCTTGCG

Protein sequence predicted based on nucleotide sequence

GPGVGSPQSYTGKEPSALRASQYGYHPYIIMMARLKQLYDIGHPIDKVEVIIQGGTFPAV
DLDYQEWFVKCAFKAMNDFHYFKDIDNLEEKLVRLILKGDKSVFKEDPEFKRAWERTHRK
PYYYLEDEQRKNEKAKVRMVGLTIETRPDWAFERQIDRMLSFGTTRVELGVQTIFNFIHE
RTKRGHGVEEIIKATQLLKDAGLKVNYHIMPGLPGSNFDRDLYTFRTIFEDPRFRPDMLK
IYPTLVTRDAPLYAWYKAGLYRPYTTEEALELLVEAYKIFPKWVRVMRIQRDIPAHLIVA
G*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a7g,S3G
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in APC66586.1
Recommended expression in:
Not Applicable