citC2 (K. pneumoniae subsp. pneumoniae MGH 78578 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  citC2
Gene Name:  putative citrate lyase synthetase (citrate (pro-3S)-lyase ligase
Original Clone ID:
APC60636_SG114_pMCSG19
Keyword:
full length
Species:
Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Type:
cDNA
Vector Name:
pMCSG19
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
KpCD00450544
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1029nts
Open reading frame: 45 to 1073

ATCTCGGGGGATTGGGTACGAGACCTGTACTTCCATCCAATGCCATGACACTGATATTAA
AACGCGTTCAGCTATTAAAAGATAAACCGCGGCGAGAGGCGATCGATCGGTTTCTCCGCC
AGCATCAACTGTCGTTAGAGGCCGACTGCGAAATGGCGATTATCGCCGAGTATCAGCAGC
GGCTGGTCGGCTGCGGTGCTATCGCCGGCAATGTGCTGAAATGCATCGCCATCGATCCCT
CGCTGCAGGGGGAGGGGCTGAGCCTTAAATTACTGACCGAGCTCCTGACGCTGGCCTATG
AGCTGGGGCGCAGCGAACTGTTTTTGTTCACTAAACCTTGCAATGCCGCGTTATTTTCCG
GCGCCGGCTTCTGGCCGATAGCCCAGGCGGGCGACCGCGCCGTGCTAATGGAAAATAGCC
GCGAACGGCTGACTCGTTACTGTCGACAGCTGGCGATGTACCGTCAGCCGGGAAGAAAAA
TCGGCGCTATCGTGATGAATGCCAATCCCTTCACCCTCGGCCACCGCTGGTTGGTAGAAC
AGGCGGCCAGCCAGTGCGACTGGCTGCATCTGTTTGTGGTCAAAGAAGATGCGTCCTGCT
TTTCCTATCACGATCGCTTCAAGCTCATTGAACAGGGGATTACCGGCATCGATAAGGTGA
CGCTGCATCCCGGTTCGGCGTATCTGATCTCGCGGGCGACGTTCCCCGGCTATTTCCTGA
AAGAGCAGGGGGTGGTTGATGACTGCCACAGCCAGATTGACCTGCAGCTCTTCCGCGAGC
GCCTGGCCCCGGCGCTGCAGATTACCCATCGCTTTGTCGGCACCGAGCCGCTGTGTCCCC
TGACCCGTAATTACAACCAGCGCATGAAGTCACTACTGGAAGCGCCAGGCGACGCGCCGC
CCATTGAAGTAGTTGAGCTTGCGCGAATCGAAAAAAATGGTGGACCCGTGTCGGCCTCCC
GAGTGCGCGAACTCTATCGACAGCGCAACTGGCAGGCGGTCGCGGCGCTGGTACCGCCGG
GAACCCTCTCTTTTCTGATGCAACTGGCGGAAAGCGAACATCAAACCGCCTAACATTGGA
AGTGGATAACGGTAA

Protein sequence predicted based on nucleotide sequence

MTLILKRVQLLKDKPRREAIDRFLRQHQLSLEADCEMAIIAEYQQRLVGCGAIAGNVLKC
IAIDPSLQGEGLSLKLLTELLTLAYELGRSELFLFTKPCNAALFSGAGFWPIAQAGDRAV
LMENSRERLTRYCRQLAMYRQPGRKIGAIVMNANPFTLGHRWLVEQAASQCDWLHLFVVK
EDASCFSYHDRFKLIEQGITGIDKVTLHPGSAYLISRATFPGYFLKEQGVVDDCHSQIDL
QLFRERLAPALQITHRFVGTEPLCPLTRNYNQRMKSLLEAPGDAPPIEVVELARIEKNGG
PVSASRVRELYRQRNWQAVAALVPPGTLSFLMQLAESEHQTA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC60636
Recommended expression in:
Not Applicable