CD1414 (C. difficile 630 )
In pMCSG19B (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CD630_14140
Gene Name:  oxidoreductase Fe-S subunit
Original Clone ID:
APC62624.1_SG306_pMCSG19B
Keyword:
partial cds
Species:
Clostridioides difficile 630
Type:
cDNA
Vector Name:
pMCSG19B
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jessica Bearden
PSI
Midwest Center for Structural Genomics
CdCD00450663
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 801nts
Open reading frame: 46 to 846

TCCTCGGGGGATTGGGTACCGAAACCTGTACTTCCATCCAATGCCTTGGATAAATTTAAA
TATGCTTTTGATAATAAAAGATATCATACTTGGAATTACTATCTTAGAAATACTTTTGGA
GAAAAAGTTTTTAAGGTATCTATAAATGCAGGATTTACTTGCCCAAACATTGATGGAAGT
CTTGGTTATGGCGGATGTACCTATTGTAGCAAAGAAGGCTCAGGTGATTTTGCTGGAAAC
CCTAAAGATAATCTTATCAGTCAATTTTATAATATAAAAGAAATGATGTTAAAAAAATGG
CCTCATGCAAAGTATATTGGCTACTTTCAAGCCTATACAAATACGTATGCTCCTCTTGAA
GTTTTAAAAGAAAAATATGAGACTATATTAGAACTTGAAGATGTCATAGGTCTTTCAATA
TCCACAAGACCTGACTGTTTGCCAGATGATATTGTAGAATACTTGTCTGAACTAAATAAA
AGAACCAATTTATGGGTTGAACTTGGACTTCAAACAATTCATGATTCTACATCTAAAATT
ATAAATAGAGGTCATGATTATAAAACATTTGTTGAAGGTGTTGAAAAATTAAAGTCTAAA
AATATAAAGGTTGTCGTACACATTATAAATGGTCTTCCTGGTGAAGATTATAATATGATG
ATGGAAACAGCAAAGGCAGTTGGTAAAATGGGTGTAGACGGTATAAAAATCCATCTATTG
CATGTTATAAAAGATACTCCTATGGAAAAGATGTTACAAAATGGTATGTTAACTCTTATG
GAACAAGATGAATATATCAATCTTGTCTGTGACCAATTAGAAATACTTCCTGAAACTATG
ATATAACATTGGAAGTGGATAACGGA

Protein sequence predicted based on nucleotide sequence

LDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDGSLGYGGCTYCSKEGSG
DFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYAPLEVLKEKYETILELEDV
IGLSISTRPDCLPDDIVEYLSELNKRTNLWVELGLQTIHDSTSKIINRGHDYKTFVEGVE
KLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVDGIKIHLLHVIKDTPMEKMLQNG
MLTLMEQDEYINLVCDQLEILPETMI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in APC62624.1
Recommended expression in:
Not Applicable