Gene:
Gene Symbol:
PEPE_0936
Gene Name:
N6-adenine-specific DNA methylase
Original Clone ID:
APC39070_SG262_pMCSG19B
Keyword:
full length
Species:
Pediococcus pentosaceus ATCC 25745
Type:
cDNA
Vector Name:
pMCSG19B
Format:
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jodi Carroll
PSI
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
PpCD00450815
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1131nts
Open reading frame: 46 to 1176
TTTCTCGGGGGATTGGGTACGAAACCTGTACTTCCATCCAATGCCATGAAGAAATTCAAT
TTAATTGCCACAATGGCAAGTGGTTTAGAAAGTTTAACAAAAAATGAATTAAAAGATTTG
GGTTATGAAGTTCAAACTGAAAATGGTCGGGTTCGTTTTGAAGGGACCTTTGAAGATATT
ATTAAAACTAATTTATGGTTAAGAACCGCTGACCGAATTAAAATAGTTGTTGGTGAGTTT
GAAGCTAAAACGTTCGATGATTTATTCGAATCTGTAAAGGCATTGCCTTGGGACGAATTG
ATCCCTGTGGATGGAGCCTTTCCAGTTAGTGGCAAATCTAAAAAATCAACTCTTTTTAGT
GTTCCGGATGTTCAAGCTATTACTAAAAAAGCGGTTGTTGAAAAATTACGTGAGGTTTTC
CATTGGCGTAATCAATTTCCAGAAACAGGGGCTAAATATGGTCTTGAAGTAGCCATTGAT
AAAGACCATGTCATGATTACCCTTGATACAACGGGTGATAGCTTATTCAAGCGTGGCTAC
CGTTTACATAAAGGTACTGCTCCACTAAAAGAAACTATGGCAGCCGCATTGGTTTTGCTA
ACTAACTGGTATCCAGACAAACCACTTGTGGATCCATTTTGTGGTTCTGGTACAGTTTTA
ATTGAAGCAGCCATGATTGGCCAAAATATTGCGCCGGGTTTTAACCGTGATTTTGCCTGT
GAAGACTGGCCCATTGTCGATCAAGAAATGGTAGAACGGGTTCGTGCTGCTGCAGATGAA
AAGGCCAATTACGATGTTGAATTAGACATCACTGGTTACGATATTGATCAAAATATGATT
GATATTGCAACTGAAAATGCGGAAGAAGCTGGATTATCTGGAATTATTTCCTTTAAGCAA
CAAGCAGTTAAGGACTTTAAAACTGATAAGAAGAATGGTGTTATGATTGGTAACCCTCCT
TATGGGGAACGAATGGGGGACCAAGAGTTAGTTCGAAAGATTTATCGTCAATTAGGGACT
GCAACGGGATCGCTAACTACCTGGAGTCGTTATTTTATCACGAGTGATTTAGATTTTGAA
AATTACTATGGTCAAAAAGCCACTAAAAAACGTAAATTATATAATGGTGCTTTGAGAACC
GATTATTTCCAATTCTGGGGTAAACGTGAAAGGTAACATTGGAAGTGGATAACGGTAA
Protein sequence predicted based on nucleotide sequence
MKKFNLIATMASGLESLTKNELKDLGYEVQTENGRVRFEGTFEDIIKTNLWLRTADRIKI VVGEFEAKTFDDLFESVKALPWDELIPVDGAFPVSGKSKKSTLFSVPDVQAITKKAVVEK LREVFHWRNQFPETGAKYGLEVAIDKDHVMITLDTTGDSLFKRGYRLHKGTAPLKETMAA ALVLLTNWYPDKPLVDPFCGSGTVLIEAAMIGQNIAPGFNRDFACEDWPIVDQEMVERVR AAADEKANYDVELDITGYDIDQNMIDIATENAEEAGLSGIISFKQQAVKDFKTDKKNGVM IGNPPYGERMGDQELVRKIYRQLGTATGSLTTWSRYFITSDLDFENYYGQKATKKRKLYN GALRTDYFQFWGKRER*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC39070
Recommended expression in:
Not Applicable
Vector Name:
pMCSG19B
Synonyms:
''
Sequencing Primer:
Forward:
ANForward
Reverse:
T7 terminator
Description:
Vector for solubility of insert and for purification, with T7 promoter, adds N-terminal MBP, TVMV cleavage site, His tag, and TEV protease cleavage site; ampicillin resistance; ligation independent cloning (LIC).
Comments:
Similar to pMCSG19C except location of the TVMV CDS. See http://bioinformatics.anl.gov/mcsg/technologies/vectors.html for more details
Size (bp):
7468
Parent Vector:
None
Properties:
bacterial expression
Author Name:
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI
Midwest Center for Structural Genomics
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
pBR322 origin of replication
5432
5432
gene fragment
TVMV cds
TVMV protease coding sequence
1881
2591
promoter
T7
T7 promoter
1515
1531
protease cleavage site
TVMV
TVMV cleavage site
286
306
protease cleavage site
TEV
TEV protease cleavage site
223
243
recombination site
LIC
Ligation Independent Cloning (LIC) recombination site
204
235
repressor binding site
lac operator
LacO fragment
1490
1512
repressor binding site
tet operator
TetO fragment
2610
2765
repressor protein gene
LacI
LacI cds
3072
4031
selectable marker
AmpR
Ampicillin resistance
6220
7080
tag
His
N terminal His tag
268
285
tag
MBP
Maltose binding protein tag
337
1143
trxn termination sequence
T7 term
T7 termination sequence
26
72
