Detailed Vector Information: pMCSG53

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pMCSG53
Source:
Midwest Center for Structural Genomics
Description:
Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis tag; Contains rare argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.
Comments:
None
Publications:
PMID: 24057978
Title: New LIC vectors for production of proteins from genes containing rare codons
Authors:
Midwest Center for Structural Genomics
Midwest Center for Structural Genomics
PSI:Biology
EvNO00450863
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Special MTA: PSI

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.