Bacterial expression vector with a T7 promoter and TEV-cleavable N-terminal 6xHis tag; Contains rare argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.
Comments:
1 kb of sequence (including the rop repressor) removed from pMCSG52 by digestion with PshAI and Tth111I, followed by treatment with Klenow, dNTPs and T4 DNA ligase
Size (bp):
4808
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Argonne National Laboratory