hflK (Pseudomonas aeruginosa PAO1) in pMCSG32

Explanation of Terms

Gene:
Gene Symbol:  hflK
Gene Name:  protease subunit HflK
Original Clone ID:
APC37761.2_SG235_pMCSG32
Keyword:
partial cds
Species:
Pseudomonas aeruginosa PAO1
Type:
cDNA
Vector Name:
pMCSG32
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Robert Jedrzejczak
PSI
Midwest Center for Structural Genomics
PaCD00452292
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 879nts
Open reading frame: 1 to 879

GCGGTCATCCTGCGCTTCGGCAAGTATTACGAGACGGTCGGTCCCGGCCTGAACTTTTAT
TTCCCGCCGATCGACAAGCGCTTCCAGGAGAACGTCACCCGCGAGCGTGCGTACAGCAAG
CAGGGGCAGATGCTCACCGAGGACGAGAACATCGTCGAAGTGCCGCTGACCGTGCAGTAC
AAGATCAGCAACCTGCAGGACTTCGTGCTCAACGTCGACCAGCCCGAGGTCAGCCTGCAG
CAGGCGACCGAGAGCGCGCTGCGGCATGTCGCCGGCTCCACCACCATGGACCGGATCCTC
ACCGAAGGCCGCGAGCAGATGGCGACCGAGGTGCGCGAGCGCCTGCAACGCTTCCTGGAT
ACCTACAGGACCGGTATCACCGTGACCCAGGTGAACATCCAGAGCGCCCAGGCACCGCGT
GAGGTGCAGGAAGCGTTCGACGACGTGATCCGGGCCCGCGAGGACGAGCAGCGCGAGAAG
AACCAGGCCGAAGCCTATGCCAACGGCGTGGTGCCGGAGGCACGTGGCCAGGCCCAGCGC
ATCATCGAAGAAGCCAACGGTTACCGCGACGAGGTGATTTCGCGCGCCCAGGGCGAGGCG
GATCGCTTCTCCAAGCTGCTGGTGGAATATCGCAAGGCTCCGGAAGTGACGCGCGAGCGT
CTGTACCTGGACACCATGCAGGAAGTCTTCAGCCAGACCAGCAAGGTGCTGGTGACCGGT
CAGCAGGGGCAGAACAACCTGCTCTACCTGCCGCTGGACAAGATGATCGATGGTCGTAAC
GCCGCGCCCGCTACGGGGGCCGGTGCTTCCGCCGGTGGTGGCAGCGCCAGCGCTTCCGAC
CTGGGGTCGCGGATCGTCAACGATCTGCGCCAGCAAGACGCTGGGGAGAACCTGTACTTC
CAATCCGCCGGGCACCACCATCATCATCAT

Protein sequence predicted based on nucleotide sequence

AVILRFGKYYETVGPGLNFYFPPIDKRFQENVTRERAYSKQGQMLTEDENIVEVPLTVQY
KISNLQDFVLNVDQPEVSLQQATESALRHVAGSTTMDRILTEGREQMATEVRERLQRFLD
TYRTGITVTQVNIQSAQAPREVQEAFDDVIRAREDEQREKNQAEAYANGVVPEARGQAQR
IIEEANGYRDEVISRAQGEADRFSKLLVEYRKAPEVTRERLYLDTMQEVFSQTSKVLVTG
QQGQNNLLYLPLDKMIDGRNAAPATGAGASAGGGSASASDLGSRIVNDLRQQD

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g4t,V2F;c57t,F19F;c60t,Y20Y
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Applicable
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in APC37761.2
Recommended expression in:
Not Applicable