PSPTO_3318 (P. syringae pv. tomato str. DC3000 )
In pMCSG7 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PSPTO_3318
Gene Name:  beta-glucosidase
Original Clone ID:
APC63610.1_SG314_pMCSG7
Keyword:
partial cds
Species:
Pseudomonas syringae pv. tomato str. DC3000
Type:
cDNA
Vector Name:
pMCSG7
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Shonda Clancy
PSI
Midwest Center for Structural Genomics
PsCD00452548
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1290nts
Open reading frame: 35 to 1324

GGGTACCGAGAACCTGTACTTCCAATCCAATGCCCCGCTGACCACCCACAACATGATCTT
CTCGACCAGCAACAAGGTCGCTCTGAATGCTGCGCGTGAAGGCATCGTGCTGCTGAAAAA
CCAGGGCGATCTGTTGCCACTGGACAAGCAGAAGGTCAAGAAAATCGCCGTCGTCGGCAC
CCTGGCCAAATATGCACCACCGACCGGTTTCGGTAGCGCCAATGTCATGGCCAGCCATTA
CGTCAGCGAGTTGAGCGGCCTGCAGCAAATGGCACCCAACGCCAAGGTCGAGTTCATCGA
TGGCCTGTCGCTGGACCCAAGCACCTCTGCCTGGAACACCACTGACGCCGCTGGCAACAG
TGTTCAGGGCATGAAGGTCGAATACTTCAGCAACACCAACTGGTCTGGCGATGCAGCGGT
CACCCGTACCGAGCAGCACGTTGACCTGGACTGGGCCAACGACAAGAACCTGCCGTTCGA
GAGCAACACCTCAACGTCCGATCCGTACACCACCAAAGGCTCGACCGCTGGTGAGCTGAA
CGGTGACACGTCTTCGACCTCGATCCGCTACACCGGCAAGATCACCCCGACCCAGAGCGG
CGAACAGGTGTTCAAGGTGCGTGCCGACGGCGCTGTGCGCCTGTGGGTCAACGGCAAGAA
AATCATCGACAACGGTGACGGCAAGCCATTGCCGGGCAACAGCATCCCGCCGACCATTCC
AGAGTTCGCCAAGATCAATCTGGAAGCAGGCCAGTCCTACGACGTGAAGCTTGAGTACTC
GCGTCGCGCCGGGTACCTGTCGACCATGGGTGGTCTGGTCGGTGTGCAGTTGAGCTCCGC
TTCGCTGAACGCGCCTCAGGACCTGTCCGGTTATGACGCGGTTGTGGTTGCAGTGGGTAA
CAGCAACGAATACGAAGGTGAAGGTTTCGACCACAGCTTCGATCTGCCCGAGTTCCAGAA
CGAACTGATCCAGAACATCGCCAAGGTCAACCCGAACACCGTGGTGACCATGTATGGCGG
TACCGGCCTGAAGATGAGCGACTGGATCGAACAGGTTCCGGTAGCGCTGCACGCCTTCTA
CCCGGGTCAGAACGGTGGTCAGGCCCTGGCCGAAATTCTGTTCGGCAAGATCAACCCGTC
GGGCAAGCTGCCGATCAGTATCGAACGCAACATCGAAGACAACCCGGCCTACGCCTCCTT
CCCGAAATTCGACAACCAGAACACGCTGGCTGAAATGGATTACAAGGATGACCTGATGCT
GGGTTATCGCGGTTACGAGAAGAAAGGCATCAAGCCGCTTTACCCGTTCGGTTACGGCCT
GTAACATTGGAAGTGGATAACGGATCCGAATTCGA

Protein sequence predicted based on nucleotide sequence

PLTTHNMIFSTSNKVALNAAREGIVLLKNQGDLLPLDKQKVKKIAVVGTLAKYAPPTGFG
SANVMASHYVSELSGLQQMAPNAKVEFIDGLSLDPSTSAWNTTDAAGNSVQGMKVEYFSN
TNWSGDAAVTRTEQHVDLDWANDKNLPFESNTSTSDPYTTKGSTAGELNGDTSSTSIRYT
GKITPTQSGEQVFKVRADGAVRLWVNGKKIIDNGDGKPLPGNSIPPTIPEFAKINLEAGQ
SYDVKLEYSRRAGYLSTMGGLVGVQLSSASLNAPQDLSGYDAVVVAVGNSNEYEGEGFDH
SFDLPEFQNELIQNIAKVNPNTVVTMYGGTGLKMSDWIEQVPVALHAFYPGQNGGQALAE
ILFGKINPSGKLPISIERNIEDNPAYASFPKFDNQNTLAEMDYKDDLMLGYRGYEKKGIK
PLYPFGYGL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a23t,N8I;a25t,g26t,S9F
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in APC63610.1
Recommended expression in:
Not Applicable