AT1G61990 (A. thaliana Columbia )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  AT1G61990
Gene Name:  mitochondrial transcription termination factor family protein
Original Clone ID:
5426.1.64_GO.4232
Keyword:
full-length cds
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
AtCD00452658
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1263nts
Open reading frame: 17 to 1279

CAAAAAAGCAGGCTCCGAAAACCCGTACTTCCAGTCCTATTCTCCGATTCTCCACGGAAG
AAGGTCGGTCCAATCGCAGAAATGCTCGAGTTTGAGCGTTTCAGTGCAAAATGCATTCGT
TTTGTCCAATTCCTTCTCTTCTGCTAGAGCTGCTGATGTGAGCATTAGAGATGGTCGAAA
AGGGAAGAACTTTACAGTCTCTTACCTCGTTGATTCATTGGGTTTATCTAAAAAGCTCGC
TGAATCCATCTCAAGGAAAGTTAGCTTTGAGGACAAGGTCAATCCTGATTCTGTTCTAAG
TCTGTTTAGAAGTTACGGGTTCACAGATTCTCAGATCTCTACCATCATTACGGATTATCC
GTTATTGCTTGTAGCAGATGCTAAGAAAGCACTTGGTCGCAAGCTTCAGATTCTGCAGTC
TAGAGGAGCTTCAAGCTCTGAGATCACTGAGATTGTTTCTACAGTACCGAGAATCTTAGG
AAAGAAATCTATTACTGTATACTATGATGCTGTCAAAGACATTATAGTAGCCGATACGAG
TTCGAGTTATGAACTCCCACAGGGTTCTCAGGGGAATAAAATCCGAAATGTATCGGCTTT
GAGAGAATTAGGCATGCCTTCTCGGTTGTTATTACCCTTGCTCGTCTCCAAATCGCAGCC
TGTGTGTGGAAAAGAAAATTTTGATGCATCCCTTAAGAAGGTTGTTGAGATGGGGTTTGA
TCCCACCACCACTAAATTTGTTTTAGCTTTGCGGATGCTTTACCAAATGAGCGAGAAAAC
GATTGAAGAAAAAGTTGTAGTCTTTAGAAGTTTAGGTTTTACTGTGGATGATGTATGGGA
AATTTTCAAGAAGACTCCTTCTGTTTTGAAAGTCTCCAAGAAGAAGATATTGAAGTCGGC
TGAAACGTTTCTAGACCTAGGATATAGCAGAGCTGAGTTTCTGATGATGGTTAAGCGTTA
TCCTCCTTGCATTGAATATTCTGTAGAGTCTGTTAAAAAGAAGAATGAGTTTCTGGTGAA
GAAGATGAAATGGCCACGAAACGCCTTGGTTTTGCACCCTCAGGTGTTTGGATACAGCAT
GGAGAAGAGGATTATACCAAGGTGTAACATACTCGAAGCTCTCTTGTCGAAAGGACTGCT
CAGAAAGGGAAGTGAACTACCTGCAGTGTCCTCTGTTTTGTCGTGTACCGATGAAGGATT
TTTAGACAGGTATGTGATGAAGCACAACGAGCTGGTGCCTACGTTGATGGCTATATTCAC
CAAAGGTAGTGTTTCATAGTAGGACC

Protein sequence predicted based on nucleotide sequence

ENPYFQSYSPILHGRRSVQSQKCSSLSVSVQNAFVLSNSFSSARAADVSIRDGRKGKNFT
VSYLVDSLGLSKKLAESISRKVSFEDKVNPDSVLSLFRSYGFTDSQISTIITDYPLLLVA
DAKKALGRKLQILQSRGASSSEITEIVSTVPRILGKKSITVYYDAVKDIIVADTSSSYEL
PQGSQGNKIRNVSALRELGMPSRLLLPLLVSKSQPVCGKENFDASLKKVVEMGFDPTTTK
FVLALRMLYQMSEKTIEEKVVVFRSLGFTVDDVWEIFKKTPSVLKVSKKKILKSAETFLD
LGYSRAEFLMMVKRYPPCIEYSVESVKKKNEFLVKKMKWPRNALVLHPQVFGYSMEKRII
PRCNILEALLSKGLLRKGSELPAVSSVLSCTDEGFLDRYVMKHNELVPTLMAIFTKGSVS
*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t8c,L3P;t29c,L10P;
t39c,H13H;t50c,L17S;
t71c,L24S;t81c,S27S;
t102c,F34F
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.4232
Recommended expression in:
Not Applicable