Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
C-His_Rv1414
Keyword:
None
Species:
Mycobacterium tuberculosis H37Rv
Type:
cDNA
Vector Name:
pET28b-C-His
Format:
FUSION
Source:
Integrated Center for Structure and Function Innovation
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Integrated Center for Structure and Function Innovation
Integrated Center for Structure and Function Innovation
MtCD00452691
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 399nts
Open reading frame: 1 to 399
GTGCTCGGTGACGCCCAGCAACTGGAACTCGGGCGCTGCGCGCCGGCGGACATCGCGCTG
ACCGTTGCCGCCACCGTAGTGAGCCGCCAGGACTGCAGGTCCGGCTTGCGCCGAATTGTC
CTTGACTGCGGTAGCAAGATTCTCGGCAGCGATCGTCCGGCCTGGGCGACTGGGTTCGGC
CGTCTGATCGACCACGCCGATGCGCGCATCGCGGCGCTGTCGGAGCATCACGCCACCGTT
GTCTGGCCCGACGACGCCCCGCTCCCGCCGGTGGGAACACGTCTGCGGGTGATTCCCAAC
CACGTGTGCCTGACCACCAACCTCGTAGATGATGTCGCCGTGGTGCGCGACGCAACCCTG
ATTGATCGCTGGAAAGTCGCCGCCCGCGGTAAGAACCATGGATCCCACCACCACCACCAC
CACTAACTCGAGCACCACCACCACCACCAC
Protein sequence predicted based on nucleotide sequence
VLGDAQQLELGRCAPADIALTVAATVVSRQDCRSGLRRIVLDCGSKILGSDRPAWATGFG RLIDHADARIAALSEHHATVVWPDDAPLPPVGTRLRVIPNHVCLTTNLVDDVAVVRDATL IDRWKVAARGKNH
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g1a,V1M
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in C-His_Rv1414
Recommended expression in:
Not Applicable
Vector Name:
pET28b-C-His
Synonyms:
C-His pET28b
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Expression vector with T7 promoter, adds C-terminal His tag; kanamycin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
5277
Parent Vector:
None
Properties:
cell-free expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name:
Integrated Center for Structure and Function Innovation
Publications:
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
promoter
T7
T7 promoter
0
0
tag
His
C-terminal His tag
5056
5073
trxn termination sequence
T7 term
T7 terminator
5167
5171
