Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
CSMP-3154
Keyword:
None
Species:
Escherichia coli
Type:
cDNA
Vector Name:
PCDF Bravo
Format:
FUSION
Source:
Center for Structures of Membrane Proteins
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of California San Francisco
William Harries
PSI
Center for Structures of Membrane Proteins
William Harries
PSI
Center for Structures of Membrane Proteins
EcCD00452777
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1533nts
Open reading frame: 47 to 1579
CGACAAGGGTGCACTTGAAGTCCTCTTTCAAGGACCGAGCAGCCCCTCACGAATTGGCAT
ACCAAGAGAACGGTTAACCAATGAAACCCGTGTTGCAGCAACGCCAAAAACAGTGGAACA
GCTGCTGAAACTGGGTTTTACCGTCGCGGTAGAGAGCGGCGCGGGTCAACTGGCAAGTTT
TGACGATAAAGCGTTTGTGCAAGCGGGCGCTGAAATTGTAGAAGGGAATAGCGTCTGGCA
GTCAGAGATCATTCTGAAGGTCAATGCGCCGTTAGATGATGAAATTGCGTTACTGAATCC
TGGGACAACGCTGGTGAGTTTTATCTGGCCTGCGCAGAATCCGGAATTAATGCAAAAACT
TGCGGAACGTAACGTGACCGTGATGGCGATGGACTCTGTGCCGCGTATCTCACGCGCACA
ATCGCTGGACGCACTAAGCTCGATGGCGAACATCGCCGGTTATCGCGCCATTGTTGAAGC
GGCACATGAATTTGGGCGCTTCTTTACCGGGCAAATTACTGCGGCCGGGAAAGTGCCACC
GGCAAAAGTGATGGTGATTGGTGCGGGTGTTGCAGGTCTGGCCGCCATTGGCGCAGCAAA
CAGTCTCGGCGCGATTGTGCGTGCATTCGACACCCGCCCGGAAGTGAAAGAACAAGTTCA
AAGTATGGGCGCGGAATTCCTCGAGCTGGATTTTAAAGAGGAAGCTGGCAGCGGCGATGG
CTATGCCAAAGTGATGTCGGACGCGTTCATCAAAGCGGAAATGGAACTCTTTGCCGCCCA
GGCAAAAGAGGTCGATATCATTGTCACCACCGCGCTTATTCCAGGCAAACCAGCGCCGAA
GCTAATTACCCGTGAAATGGTTGACTCCATGAAGGCGGGCAGTGTGATTGTCGACCTGGC
AGCCCAAAACGGCGGCAACTGTGAATACACCGTGCCGGGTGAAATCTTCACTACGGAAAA
TGGTGTCAAAGTGATTGGTTATACCGATCTTCCGGGCCGTCTGCCGACGCAATCCTCACA
GCTTTACGGCACAAACCTCGTTAATCTGCTGAAACTGTTGTGCAAAGAGAAAGACGGCAA
TATCACTGTTGATTTTGATGATGTGGTGATTCGCGGCGTGACCGTGATCCGTGCGGGCGA
AATTACCTGGCCGGCACCGCCGATTCAGGTATCAGCTCAGCCGCAGGCGGCACAAAAAGC
GGCACCGGAAGTGAAAACTGAGGAAAAATGTACCTGCTCACCGTGGCGTAAATACGCGTT
GATGGCGCTGGCAATCATTCTTTTTGGCTGGATGGCAAGCGTTGCGCCGAAAGAATTCCT
TGGGCACTTCACCGTTTTCGCGCTGGCCTGCGTTGTCGGTTATTACGTGGTGTGGAATGT
ATCGCACGCGCTGCATACACCGTTGATGTCGGTCACCAACGCGATTTCAGGGATTATTGT
TGTCGGAGCACTGTTGCAGATTGGCCAGGGCGGCTGGGTTAGCTTCCTTAGTTTTATCGC
GGTGCTTATAGCCAGCATTAATATTTTCGGTGGCTTCACCGTGACTCAGCGCATGCTGAA
AATGTTCCGCAAAAATGGAGGGTTGGTTCCCCGTGGTAGTTCCGCTCATCACCACCATCA
TCACCATCAC
Protein sequence predicted based on nucleotide sequence
SRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLASFDDKAFVQAGAEIVEG NSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELMQKLAERNVTVMAMDSVPR ISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA IGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKEEAGSGDGYAKVMSDAFIKAEME LFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGSVIVDLAAQNGGNCEYTVPGEI FTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNLLKLLCKEKDGNITVDFDDVVIRGVTV IRAGEITWPAPPIQVSAQPQAAQKAAPEVKTEEKCTCSPWRKYALMALAIILFGWMASVA PKEFLGHFTVFALACVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGQGGWVSF LSFIAVLIASINIFGGFTVTQRMLKMFRKNG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
streptomycin
Bacterial Selection Condition:
streptomycin
Growth Condition:
LB with 50ug/ml streptomycin
Comments:
None
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in EC_P3_114
Recommended expression in:
Not Applicable
Vector Name:
PCDF Bravo
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter, N-terminal His tag and C-terminal Flag tag; ligation independent cloning (LIC) clone; streptomycin resistance in bacteria.
Comments:
None
Size (bp):
3636
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
William Harries
Center for Structures of Membrane Proteins
PSI
Center for Structures of Membrane Proteins
PSI
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial operon
lac operator
lacO
3
25
bacterial origin
CloDF13
CloDF13 origin
1466
2204
gene fragment
LacI
LacI transcriptional represso
2417
3373
promoter
T7
T7 Promoter
3621
3636
ribosome binding site
RBS
RBS-1: ribosome binding site
58
63
selectable marker
StrepR
streptomycin resistance gene
535
1326
tag
His
N-terminal 10xHis tag
170
199
tag
Flag
C-terminal Flag tag
80
103
trxn termination sequence
T7 term
T7 terminator
306
324
