Sthe_2607 (S. thermophilus DSM 20745 )
In pMCSG7 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Sthe_2607
Gene Name:  Radical SAM domain-containing protein
Original Clone ID:
APC100703.102
Keyword:
None
Species:
Sphaerobacter thermophilus DSM 20745
Type:
cDNA
Vector Name:
pMCSG7
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Shonda Clancy
PSI:Biology
Midwest Center for Structural Genomics
StCD00454211
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1440nts
Open reading frame: 45 to 1484

GTGTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGTCGACTGCGACCC
AGCAGAACGCAGGTGCCCGAGCACCGATTGGCGACCTCACCGCCCCCGGCGCCATCCTGC
TCGTCTCGTGCTACGAACTTGGCCACCAGCCGTTGAACCTGGCCTTCCCGCTGGCCTACC
TGACCCAAGCGGGCTATTCCCCGGTGGCCGTCGACACGGCGGTCGAACCACTCGATGACG
CGGCGATCGCGGCCGCGCGGGTGGTGGCGATTTCCGTCCCGATGCACACCGCGCTGCGGC
TGGGCACACAGGTCGCCCGCCGGGTTCGCCAGATTAACCCGGACGCGGTCATCGGCTTCT
ACGGCCTCTACGCGTGGCTCAACGCCGACTACCTGCTCCGCGAGCACGGCGACTTCGTGA
TCGGCGGCGAGTATGAGCGCCCGCTGCTCGACTTCGTGCGCGCGCTTGCGGCCGGCAGCG
ACGAGCCGGTGGAGGGCGTCTCCACCCGCGATTACCGGGTGCCTCCGTCGCTCGCGCGCA
TCGACTTTCCCATGCCGGAGCGGGCCAGCCTTCCGGCGATGAAGCGCTATGCGCACCTCA
TGATCGACGGGACGGCGATCCCGGCCGGGTACACCGAGACGACCCGCGGCTGCCACCACA
CCTGTCTGCACTGCCCGGTGGTGCCGATCTACCGCGGGCGGTTCTTCGCCATTCCGCGCG
AGGTCGTGCTGGAGGACATCCGACGGCAGGTGCGCCAGGGCGCCGGGCATATCACCTTTG
GCGACCCCGACTTTCTGAACGGCCCGACCCACGCACTGCGCATCTGCCGGGCGATGCATG
CGGAGTTCCCGCACGTCACCTTCGACATGACCACGCGGATCGAGCACATCTTGAAGCATC
GCGACCTCATCCCCGAGTTCGCGCAGCTCGGGTGCGTCTTCGTCGTCTCGGCGGTGGAGT
CGGTCAGCGAGATGGTGCTCGACCGCATCGACAAGGGCCATACGAAGGCGGACGTCGTCG
AGGCGCTGCGTATTCTGGAGAGCGCGGGCATCGCCATGCGCCCGTCGCTGCTCCCCTTCA
CGCCGTGGACCACGATGGAGGATTACCTCGAACTCCTGCGGTTCGTCGAGGAATACGACC
TGATCGACAACATCGATCCGGTGCACTTCTCGATCCGCCTCCTGGTGCCGCCCGGATCCG
CCCTGCTGGACCGTCCAGAAACCAAGGAGTGGCTCGGCCCACTCGATGAGGCGGCCTTCA
CCTACACCTGGCGCAGCCCCGATCCGCGGCTGGATGCCCTCCAGCAGGAGGTTGCCCGCA
TCGCGGAGGCAGGCGAGGCTGCCGGTCAGGACTACGAGGAGACCTTCGCCCAGATTAAGG
CTGCTGCCTACGCCGCGGCCGGCCTGGAGGTGCCCCCGCCGGTGCGGCGTGACACCGGCG
TGCGCCGCCGGCCACCCCGCCTCAGCGAATCCTGGTTCTGTTAACATTGGAAGTGGATAA
CGGATCCGAATTCGAGCTC

Protein sequence predicted based on nucleotide sequence

MSTATQQNAGARAPIGDLTAPGAILLVSCYELGHQPLNLAFPLAYLTQAGYSPVAVDTAV
EPLDDAAIAAARVVAISVPMHTALRLGTQVARRVRQINPDAVIGFYGLYAWLNADYLLRE
HGDFVIGGEYERPLLDFVRALAAGSDEPVEGVSTRDYRVPPSLARIDFPMPERASLPAMK
RYAHLMIDGTAIPAGYTETTRGCHHTCLHCPVVPIYRGRFFAIPREVVLEDIRRQVRQGA
GHITFGDPDFLNGPTHALRICRAMHAEFPHVTFDMTTRIEHILKHRDLIPEFAQLGCVFV
VSAVESVSEMVLDRIDKGHTKADVVEALRILESAGIAMRPSLLPFTPWTTMEDYLELLRF
VEEYDLIDNIDPVHFSIRLLVPPGSALLDRPETKEWLGPLDEAAFTYTWRSPDPRLDALQ
QEVARIAEAGEAAGQDYEETFAQIKAAAYAAAGLEVPPPVRRDTGVRRRPPRLSESWFC*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC100703
Recommended expression in:
Not Applicable