KPN_00513 (K. pneumoniae subsp. pneumoniae MGH 78578 )
In pMCSG7 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  KPN_00513
Gene Name:  beta-fructofuranosidase; sucrose-6-phosphate hydrolase
Original Clone ID:
APC100108.102
Keyword:
None
Species:
Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Type:
cDNA
Vector Name:
pMCSG7
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jessica Bearden
PSI:Biology
Midwest Center for Structural Genomics
KpCD00454232
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1401nts
Open reading frame: 43 to 1443

GTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATGTCATTACCGTCACGC
CTGCCTGCGATCCTGCAGGCCGTTATGCAGGGCCAGCCGCAGGCGCTGGCCGACAGCCAT
TATCCGCAATGGCATCTGGCGCCGGTCAACGGACTGCTGAACGATCCTAACGGCTTTTGC
CAGGTCGCCGGGCGTTACCACCTGTTTTATCAGTGGAACCCGCTCGCCTGCGACCATACC
TATAAGTGCTGGGGACACTGGAGCTCTGCCGATCTGCTGCACTGGCGGCACGAACCTATC
GCCCTGATGCCGGATGAAGAGTATGACCGCAACGGCTGCTACTCTGGCAGCGCGGTCGAG
TTCGAGGGCGCCCTGACTCTGTGCTACACCGGCAACGTGAAATTCCCCGACGGCGGGCGC
ACCGCCTGGCAATGTCTGGCGACCGAGAATGCCGATGGCACCTTCCGCAAGCTGGGGCCG
GTGCTGCCGCTGCCAGAAGGCTATACCGGCCATGTGCGCGACCCTAAAGTGTGGCGGCAG
GACGGGCGCTGGTACATGGTTCTTGGGGCGCAGGATGTGCAACAGCGCGGCAAAGTGCTG
CTGTTTACCGCCAGCGACCTGCGGGAGTGGCGCCTGGTGGGCGAGATTGCCGGGCACGAC
GTAAACGGCCTGGCGAACGCCGGCTACATGTGGGAGTGCCCGGATCTCTTTCCGCTGGCG
GACACCCACCTGCTGATCTGCTGCCCGCAGGGGCTGGCCCGCGAAGCGCAGCGCTTTCTC
AATACCTATCCGGCGGTGTGGATGGCAGGCCGCTTCGACGCCGAACGCGGGATCTTCGAC
CACGGCCCGCTGCACGAGCTGGACAGCGGATTTGAGTTCTACGCGCCGCAGACCATGCAG
GCCGATGATGGCCGCCGCCTGCTGGTTGGCTGGATGGGCGTCCCCGACGGGGACGAGATG
CATCAGCCCACCCGCGCGCAGGGCTGGATCCATCAGATGACCTGCGTGCGTGAGCTGGAG
TGGCAGGCTGGCACTCTGTATCAGCGTCCGCTGCGCGAGCTGGTCGCCCTGCGCGGGGAA
GCCCAGGGCTGGTGCGGACAGACCCTGCCCCTCGCCCCGATGGAGCTGGCCTTTGACATC
GCCCCCAACAGCACGCTGGGGCTGGACTTTGCCGGCGCCCTGCAGCTCACCGTCAATCGC
GACGGCCTGCGTCTGTCGCGTCGCGGCCTGCAGACGGCGGAGCTGCATCACCGCTACTGG
CGCGGCGAGGCGCGACGCCTGCGGATCTTTATCGACCGCTCCAGCGTGGAGATTTTCATC
AACGATGGCGAGGGGGTGATGAGCAGCCGCTTCTTCCCGGGCTATCCGGGGCAGCTCATC
TTCAGCGGTGCGACGCCGGTAGCATTCTGCCGCTGGCTGCTGCGGCCATGCATGGTAGAA
TAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTC

Protein sequence predicted based on nucleotide sequence

MSLPSRLPAILQAVMQGQPQALADSHYPQWHLAPVNGLLNDPNGFCQVAGRYHLFYQWNP
LACDHTYKCWGHWSSADLLHWRHEPIALMPDEEYDRNGCYSGSAVEFEGALTLCYTGNVK
FPDGGRTAWQCLATENADGTFRKLGPVLPLPEGYTGHVRDPKVWRQDGRWYMVLGAQDVQ
QRGKVLLFTASDLREWRLVGEIAGHDVNGLANAGYMWECPDLFPLADTHLLICCPQGLAR
EAQRFLNTYPAVWMAGRFDAERGIFDHGPLHELDSGFEFYAPQTMQADDGRRLLVGWMGV
PDGDEMHQPTRAQGWIHQMTCVRELEWQAGTLYQRPLRELVALRGEAQGWCGQTLPLAPM
ELAFDIAPNSTLGLDFAGALQLTVNRDGLRLSRRGLQTAELHHRYWRGEARRLRIFIDRS
SVEIFINDGEGVMSSRFFPGYPGQLIFSGATPVAFCRWLLRPCMVE*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC100108
Recommended expression in:
Not Applicable