Gene:
Gene Symbol:
chiA2
Gene Name:
chitinase A
Original Clone ID:
APC100206.104
Keyword:
None
Species:
Streptomyces avermitilis MA-4680 = NBRC 14893
Type:
cDNA
Vector Name:
pMCSG7
Format:
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Jessica Bearden
PSI:Biology
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
SaCD00454289
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1686nts
Open reading frame: 43 to 1728
GTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGCGCATCACGATGTTC
AGACGCTCCAGACGTTCCCTCCTCGCCCTGCTGGGCTCCGCCGCCCTGGCTCTGGCCGGG
GCGGTCGCCCTCCCCGGCACGGCCCAGGCGGCCAACATCCTGTCCAATCCCGGCTTCGAG
TCGGGCAGCCTGTCCCCCTGGTCCTGCACGGGAAATCTGGGGTCGGTCGTCTCCAGTCCC
GTGCACGGCGGCTCCAAGTCCCTTGCGGGAGCCGTGAGTTCGAGTGACACCGCACAGTGC
AGCCAGACCGTCCCGGTGCAGCCGAACACGACGTACAGCCTGTCGGGCTGGGTGCGCGGC
AGCTATGTGTACCTCGGCGTGGACGGCGGCGCCTCGACCTGGACGACGTCCACGTCGGCG
TACAGCCGGCTGTCGGTCTCCTTCACCACCGGCGCCTCGCAGACCAGCGCCAAGATCTAT
GTCCACGGCTGGTACGCGCAGGGCACCTACTACGCCGACGACATCAGCCTGGACGGTCCG
GGCGGAGGCGGCTCGGACACCCAGGCGCCGACGGCTCCGGGCAGCCTCCGCTCCACCGGC
AAGACCTCGTCGAGCGCGACCCTGGCGTGGAACGCCTCGACGGACAACGTGGGCGTCACG
ACGTACGACGTCTACAGCGGCTCCAACCAGGTGCTGAGCGTCTCCGGTACGACCGCCACG
GTCAGCGGGCTGTCGCCGAGCACGGCGTACACGTTCACTGTGAAGGCGCGGGACGCGGCC
GGGAACACCTCGGCGGCCTCCAACTCCGTGTCCGTCACGACGGACGCGGGCAGCGGCGGC
GGCACCGGCTTCAAGCAGGCCGCGCCCTATCTCTACCTCGGCTGGGGCGATCCGCCGAGC
GCGACCTCGGTGATGAGCTCGACCGGCATCAAGTGGTACACGATGGCGTTCATGCTGGAC
TCCGGCGGCTGCAACCCCGCCTGGGACGGCAGCCGGCCGCTGACCGGCGGCAACGACCAG
AGTGTCATCAACTCGGTCCGCTCCGCGGGCGGTGACGTCGTCCCGTCGTTCGGCGGCTGG
TCGGGCAGCAAGCTCGGCGCCAACTGCTCCTCGGCGAGCGCGCTGGCCGGGGCGCTGCAG
AAGGTGATCGACGCCTATGGCCTCAAGGCGATCGACATGGACATCGAGAACACGGACGAG
TTCGAGAACGCCACCGTCCAGGACCGCATCCTGACCGCGCTGAAGACGGTCAAGGCCAAC
AATCCGGGCCTGCGCACGATCGTCACCTTCGGCACCACGACGACCGGCCCCACCTACTGG
GGCAACCGCCTCATCGAGCAGGCCCAGGCCCTGAACGCCGGCATCGACGTCTTCACGATA
ATGCCGTTCGACTTCGGCGGCGGCTCCGACATGTACGGCAACACGGTGAACGCGACCGAC
GGTCTGAAGGCCAAGCTCAAGTCGACGTTCGGCTGGGACGACGCCACCGCGTACTCCCAC
ATCGGCATCTCCGGCATGAACGGCCTGTCCGACCAGCAGGAGAACACGTCGCCGGCGACC
TGGACCCAGATCCGCGACTGGGCCAACTCGCACCACATCGCCCTCCTCGCCTTCTGGTCG
GTCAACCGCGACCGGCCGTGCCCCGGCGGGGGCGTGGTGAGCAACTGCTCCGGCATCAGC
CAGAACACCTGGCAGTTCACCTCGATCACGGCCGGTTTCACCGGCTGATAACATTGGAAG
TGGATAACGGATCCGAATTCGAGCTCCGT
Protein sequence predicted based on nucleotide sequence
VRITMFRRSRRSLLALLGSAALALAGAVALPGTAQAANILSNPGFESGSLSPWSCTGNLG SVVSSPVHGGSKSLAGAVSSSDTAQCSQTVPVQPNTTYSLSGWVRGSYVYLGVDGGASTW TTSTSAYSRLSVSFTTGASQTSAKIYVHGWYAQGTYYADDISLDGPGGGGSDTQAPTAPG SLRSTGKTSSSATLAWNASTDNVGVTTYDVYSGSNQVLSVSGTTATVSGLSPSTAYTFTV KARDAAGNTSAASNSVSVTTDAGSGGGTGFKQAAPYLYLGWGDPPSATSVMSSTGIKWYT MAFMLDSGGCNPAWDGSRPLTGGNDQSVINSVRSAGGDVVPSFGGWSGSKLGANCSSASA LAGALQKVIDAYGLKAIDMDIENTDEFENATVQDRILTALKTVKANNPGLRTIVTFGTTT TGPTYWGNRLIEQAQALNAGIDVFTIMPFDFGGGSDMYGNTVNATDGLKAKLKSTFGWDD ATAYSHIGISGMNGLSDQQENTSPATWTQIRDWANSHHIALLAFWSVNRDRPCPGGGVVS NCSGISQNTWQFTSITAGFTG*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g1562t,R521L
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in MCSG-APC100206
Recommended expression in:
Not Applicable
Vector Name:
pMCSG7
Synonyms:
pMCSG-7
Sequencing Primer:
Forward:
T7short
Reverse:
T7 terminator
Description:
with tag/fusion/marker assay, ligation independent cloning SspI (LIC-SspI), bacterial expression, in vitro transcription expression with AmpR and His and LIC and ori and T7 and T7 term and TEV and His 2 and LacI and lac operator; ampicillin resistance in bacterial;
Comments:
pET21 derivative; for routine HTP purification; SspI site used for LIC cloning
Size (bp):
5286
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
LIC
ligation-independent cloning (LIC) region, SspI site used
204
235
bacterial origin
ori
ColE1 pBR322-type bacterial origin of replication
3280
3280
promoter
T7
T7 promoter
360
376
protease cleavage site
TEV
TEV cleavage site cds
223
243
repressor binding site
lac operator
LacO fragment
335
357
repressor protein gene
LacI
LacI coding region
890
1849
selectable marker
AmpR
ampicillin resistance gene
4038
4898
tag
His 2
N-terminal 6xHis tag
268
285
tag
His
C-terminal 6xHis tag
140
157
trxn termination sequence
T7 term
T7 terminator sequence
26
72
