thiH (Anaerococcus prevotii DSM 20548) in pMCSG48

Explanation of Terms

Gene:
Gene Symbol:  thiH
Gene Name:  thiamine biosynthesis protein ThiH
Original Clone ID:
APC101057.105
Keyword:
None
Species:
Anaerococcus prevotii DSM 20548
Type:
cDNA
Vector Name:
pMCSG48
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Shonda Clancy
PSI:Biology
Argonne National Laboratory
Midwest Center for Structural Genomics
ApCD00454471
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1419nts
Open reading frame: 51 to 1469

CCGCGATGGCGCCGTATACACAACGAGAACCTGTACTTCCATCCAATGCCATGGTAGTAT
ATAATCCAAAATCTAGCAAGGCTAGTGAATTTATTAACCATGAAGAAATTTTAGAGACTC
TTGATTATGGTAGAGAAAACGCCCATAATAGAGAGCTTATCATAGATATTTTAAATAAGG
CAAAAAAGGCTAAGGGCCTAAGCCACAGGGAAGCCTTTGTGCTTTTATCTTGTAAGGAAG
AAGACCTAAACGCAGAGATCTTTAACCTTGCCAAAGAGCTTAAACACAAGTTTTACGCAA
ATAGAATAGTGCTTTTCGCTCCTTTGTATTTGTCTAACTATTGTGTGAATGGTTGCTCTT
ATTGTCCTTACCACGGACAAAACAGAACTATTCCAAGAAGAAAGCTAAGTCAAGAAGAAA
TTCGTGAGCAAGTTATAGCTCTACAAGACTTAGGACACAAGAGACTTGCCCTAGAAGCTG
GAGAAGATCCAGTAAATAACCCTCTAGAATACATACTAGAGTCGATAGATACAATCTATA
ATATTAAACACAAAAACGGAGCTATAAGAAGGGTAAACGTAAATATCGCAGCTACTACTG
TTGAAAATTACAGAAAGCTTCACGAGGCAGAAATCGGAACTTACATCCTCTTCCAAGAAA
CTTACAACAAGGAAAATTACGAGTCTCTCCACAAGTTTGGTCCAAAATCTAACTACGAAT
ATCACACTGAAGCAATGGATAGGGCCTTCGAGGGAGGAATCGACGATTTAGGACTTGGAG
TTTTGTATGGACTCGATTCTTATGAATATGAATTTGTTGGCCAATTAATGCACGCAGAAC
ACCTTGAGGCGAGATTTAATGTCGGCCCTCATACAATCTCTGTGCCTAGAATCCAACCTG
CTGACGATATTGATCCAAACGACTTCGACAATTCCCTATCAGATGAAATGTTTGAGAAAA
TCGTAGCCTGCATCAGAGTTGCAGCACCATATACTGGTATGATCGTATCTACTAGGGAGA
GCGAAAAGGTCCGTGCTAAGCTCTTAGACCTTGGTATTTCCCAAATCTCCGGAGGTTCCA
AGACTTCTGTAGGAGGATATACAGAAAACGAAACCAAGGGAAGTGATCAATTCGAACTAT
CAGATAACAGAACTCTAGATGAAGTAATCGACTGGCTAATATCAAAAGACCACGTACCAA
GTTTCTGTACAGCTTGCTACAGGATGGGAAGAACTGGCGAGACCTTTATGGGCATGGTCA
AAAAACACGGAATCGGTAATATTTGCCATCCAAACGCCCTCACAACTCTCGAAGAATATG
TAATAGATTACGCAAGTGATAAGACAGCCAAGGATGCCGAAGCCCTTATCCAAAGAGAGC
TAGCTAATATTCCAAATCCAGAAGTCCGTGAATCAACTAGGGAAAATCTAGAAAAAATCA
AACAAGGACAAAGAGACCTTTATATATAACATTGGAAGTGGATAACGGATCCGAATTCGA
GCTCCGTCG

Protein sequence predicted based on nucleotide sequence

MVVYNPKSSKASEFINHEEILETLDYGRENAHNRELIIDILNKAKKAKGLSHREAFVLLS
CKEEDLNAEIFNLAKELKHKFYANRIVLFAPLYLSNYCVNGCSYCPYHGQNRTIPRRKLS
QEEIREQVIALQDLGHKRLALEAGEDPVNNPLEYILESIDTIYNIKHKNGAIRRVNVNIA
ATTVENYRKLHEAEIGTYILFQETYNKENYESLHKFGPKSNYEYHTEAMDRAFEGGIDDL
GLGVLYGLDSYEYEFVGQLMHAEHLEARFNVGPHTISVPRIQPADDIDPNDFDNSLSDEM
FEKIVACIRVAAPYTGMIVSTRESEKVRAKLLDLGISQISGGSKTSVGGYTENETKGSDQ
FELSDNRTLDEVIDWLISKDHVPSFCTACYRMGRTGETFMGMVKKHGIGNICHPNALTTL
EEYVIDYASDKTAKDAEALIQRELANIPNPEVRESTRENLEKIKQGQRDLYI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC101057
Recommended expression in:
Not Applicable