Apre_1844 (Anaerococcus prevotii DSM 20548) in pMCSG48

Explanation of Terms

Gene:
Gene Symbol:  Apre_1844
Gene Name:  Radical SAM domain-containing protein
Original Clone ID:
APC101085.105
Keyword:
None
Species:
Anaerococcus prevotii DSM 20548
Type:
cDNA
Vector Name:
pMCSG48
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Shonda Clancy
PSI:Biology
Argonne National Laboratory
Midwest Center for Structural Genomics
ApCD00454490
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1362nts
Open reading frame: 49 to 1410

GATTGGTGCCGTATACAACACGAGAACCTGTACTTCCAATCCAATGCCGTGTATTTAAAG
GCTAAAAATATAAAAGCAATTGAAAAACAAAAAAATGCCAATATTTTGGTTGTTGAAAAT
AGATATGAGAAAATGGGTGAATCTCTTTATAGAATAAATAATGATGCAACAAACATAATA
CTTTGGTTAGATGGAAATCATACATTTGATGAAATTGCTAAAAAGATGTGTGATAATAAT
ACGAGCTTAATTGAAGAAGCTAAAATTAATTTAAATGAATTTTTAAATTCTTTAATTCTC
AATTATGGCATTGTTATTGATAGATTAGAAAATCCGAGATATGATGAAATAAGGGTTTTA
GGAAATGGTAAAACACAGTACCCATCAGCAATATCTATTGAAATAACACATACTTGTAAT
GTAAAGTGCTTACATTGTTATGGTGAATATTCATGCTATAATAAATTTAAAGACAATACT
GAATCGATATTTAAAGTACTAGAAGATGCAAGAAGATCTGGTACTAGAGTAGTAGAATTT
ACGGGTGGAGAAGTTACCTGTCATCCAAGATTTCTTGATATTTTAGACAAGGCATATTCT
TTAAATTATCATTTAGTAAGTATTTTATCAAATGGAACAAACTGGAATGAAGACTTGTTT
AATGAAATATATAAAAATAGGGACAAGACGGTAGTACAAATAGATCTACATGGAGACAAT
GACGATTATATTAATTGGTTTATGGGTTCTAAGATAAAAAATATTTCGAATATCATAAAA
AGTAATATAGTTAGAATTCACAAAATGGGAATATATATGAGGATTGTAACTATGATAACG
CCAAAGAATATAAATCAATTAGAAAATATTGCTGATTGGATTCATGAAAATGGCATAGAG
ACTTATGGTCTATCTTCAATTATACCAATGGGAAGAGCTACTTTAGGAGATAATAATAAT
TTATTATTAAAAACTAAAGAAGATTCTGAAAAATTGAATGAAGTTATTTCAAAAATAAAT
AATAAGTATGGATATGGTTTTCTATATCAAGTAAAGGACGGAGATCCAAACGTTAAAAAT
TGTGGAGCATTTACAAGAAATCCATCTATTACTCCAGAGGGAGATATTAAATTTTGTGCT
ATGGATGATCAATCACTTATAAAAAGCTTTGGCAATGTATTTAATACTGATATCGGTAAG
CTATATACAGAAAATTATAAAATATTAGATTTGATAAGAAATATAGAAGCACCTAGCTAT
GTAAATTCTGAGTGTGAAAATTGTGAGAAGAAATTTTTTTGTAGTGAATGCATTATAAGG
GGACTCATTGGAGCAAAAGAAATAGGAGTAGAAAATTGTGCTTGGTTTAATAATAAGTTA
AATGATGAGTTTAGGAGGCTTTTAATATAACATTGGAAGTGGATAATAA

Protein sequence predicted based on nucleotide sequence

VYLKAKNIKAIEKQKNANILVVENRYEKMGESLYRINNDATNIILWLDGNHTFDEIAKKM
CDNNTSLIEEAKINLNEFLNSLILNYGIVIDRLENPRYDEIRVLGNGKTQYPSAISIEIT
HTCNVKCLHCYGEYSCYNKFKDNTESIFKVLEDARRSGTRVVEFTGGEVTCHPRFLDILD
KAYSLNYHLVSILSNGTNWNEDLFNEIYKNRDKTVVQIDLHGDNDDYINWFMGSKIKNIS
NIIKSNIVRIHKMGIYMRIVTMITPKNINQLENIADWIHENGIETYGLSSIIPMGRATLG
DNNNLLLKTKEDSEKLNEVISKINNKYGYGFLYQVKDGDPNVKNCGAFTRNPSITPEGDI
KFCAMDDQSLIKSFGNVFNTDIGKLYTENYKILDLIRNIEAPSYVNSECENCEKKFFCSE
CIIRGLIGAKEIGVENCAWFNNKLNDEFRRLLI*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t862c,L288L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in MCSG-APC101085
Recommended expression in:
Not Applicable