Aaci_2046 (A. acidocaldarius subsp. acidocaldarius DSM 446 )
In pMCSG7 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Aaci_2046
Gene Name:  metallo-beta-lactamase superfamily hydrolase
Original Clone ID:
APC100315.102
Keyword:
None
Species:
Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
Type:
cDNA
Vector Name:
pMCSG7
Format: 
CLOSED
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Jessica Bearden
PSI:Biology
Midwest Center for Structural Genomics
AaCD00454622
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1314nts
Open reading frame: 38 to 1351

TCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGGACAAGCCAACACGCATCAC
ATTCTTCGGGGGAACGCGCACCATCGGCGGTACCATTTGCCGGGTGAAGACGCCTTCCGC
CCAGCTCGTCTTCGACATGGGGCATGTGGTTCGCCCCGCCTCGCCGATGTTCTCAGGTCC
GCTGCGCCCGCGTTCAACCGCCGACCTCCGGGCGGTCGGCATCCTTCCGGATATTCCTGA
GCTTTATACCCATGATCAACGAGGAAACTTGGCAATCTGCATAAGCCATAACCACTTGGA
TCATACCGCTCTTTTGCCTTATATGGCAAACGAAATCTCGATTTTTTCGACCAAAGAGAC
ATGTAAGATCTTGAGGCTTATCTCGGAAGCCGCCATTGAAGATCAACCTCCGCTTCGGGT
CATCGGCGTCGAACCCGAGGAATGGCGGAGCGTCGGGGATCTGCAGTTTCAATTCGTCCC
AGTCGATCACGACACGCCCGGGGCAGCAGCCATCTTCATCACCGCCCCGGATGTCCGGCT
GGTCTACACGGGAGATCTGCGGTTTCACGGATGGAGGCCCGAGGTTTCGCGCGCGTTCGT
CGAGCAGGCGCGCAGGTTCGAGCCGGACGTGCTGCTCATTGAGGGGACGCGGGCCGGGGA
CGAGGCGCGCCCGCAAGTGACGGAGCAGGAACTCCTCCGTCTGATTGTGGAGGCTATGGC
GGGCGAGGCTCGCATGGTCTATTTCAACGCGTATCCGCGCCATCCAGAGCGCGTCCTCGC
GTTCGCCCGTGCGGCTCGCGAAGCGAAGCGCTTGCCGGTCTTCGAAGCGCGAACGCTCTA
CGTGGCCTCGCACTTCACAGATGGCCTGCCCGAGGACATCCATGTCTGGGCGGACGATGA
ACTCCCTGACCCCGTCGCGAGATGGCTCAAAGACAGAGGTTTGCCTTCCCTCGAGGCCTC
CGACGTGCGGAAAGCGCCAAGTGACTTCGCCGTCGAACTCGCCTACGACCGGCTGTGGCG
GCTCATTGACATAGGCGCCGCCGCCCCAGGACTCTACATTCATTCCAACGGGGCTCCGCT
CGGGCCGTTCGATCCGGCCTGGGACAATCTCCAGCGGTGGCTCGCGCATTTCGAAGTGAC
CTTTCGCGCCATCGGCACCAGCGGGCACGCGGCGCTCGACGAGATTGAACAGGCCATCGA
GGCTATCCGCCCTCGCGTCTATCTGCCGATTCACTCGTTTCATCCTGAGCGCGTGATCGC
GTCCGGAATCCCGCGCGTTTTGCCCGAGGAGCGCGTGGATTACACGTTGTCCCATCTCCT
CGCCGCCGCGTCCGTGGCTTCCCGCGCGTAACATTGGAAGTGGATAATAA

Protein sequence predicted based on nucleotide sequence

VDKPTRITFFGGTRTIGGTICRVKTPSAQLVFDMGHVVRPASPMFSGPLRPRSTADLRAV
GILPDIPELYTHDQRGNLAICISHNHLDHTALLPYMANEISIFSTKETCKILRLISEAAI
EDQPPLRVIGVEPEEWRSVGDLQFQFVPVDHDTPGAAAIFITAPDVRLVYTGDLRFHGWR
PEVSRAFVEQARRFEPDVLLIEGTRAGDEARPQVTEQELLRLIVEAMAGEARMVYFNAYP
RHPERVLAFARAAREAKRLPVFEARTLYVASHFTDGLPEDIHVWADDELPDPVARWLKDR
GLPSLEASDVRKAPSDFAVELAYDRLWRLIDIGAAAPGLYIHSNGAPLGPFDPAWDNLQR
WLAHFEVTFRAIGTSGHAALDEIEQAIEAIRPRVYLPIHSFHPERVIASGIPRVLPEERV
DYTLSHLLAAASVASRA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in MCSG-APC100315
Recommended expression in:
Not Applicable