Bacterial expression vector with a T7 promoter and N-terminal 6xHis tag, TEV cleavable; ampicillin resistance in bacteria; Ligation Independent cloning
Comments:
None
Publications:
None
Authors:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics
Bacterial expression vector with a T7 promoter and N-terminal 6xHis tag, TEV cleavable; ampicillin resistance in bacteria; Ligation Independent cloning
Comments:
pMCSG7 digested with PshAI and BsaAI to remove a 1008 bp fragment containing the rop repressor gene and then re-ligated. Origin of replication now similar to the pUC vectors.
Size (bp):
4278
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics