MCM5 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  MCM5
Gene Name:  minichromosome maintenance complex component 5
Original Clone ID:
ccsbBroadEn_06570
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC000142.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505519
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2203nts
Open reading frame: 1 to 2203

ATGTCGGGATTCGACGATCCTGGCATTTTCTACAGCGACAGCTTCGGGGGCGACGCCCAG
GCCGACGAGGGGCAGGCCCGCAAATCGCAGCTGCAGAGGCGCTTCAAGGAGTTCCTGCGG
CAGTACCGAGTGGGCACCGACCGCACGGGCTTCACCTTCAAATACAGGGATGAACTCAAG
CGGCATTACAACCTGGGGGAGTACTGGATTGAGGTGGAGATGGAGGATCTGGCCAGCTTT
GATGAGGACCTGGCCGACTACTTGTACAAGCAGCCAGCCGAGCACCTGCAGCTGCTGGAG
GAAGCTGCCAAGGAGGTAGCTGATGAGGTGACCCGGCCCCGGCCTTCTGGGGAGGAGGTG
CTCCAGGACATCCAGGTCATGCTCAAGTCGGACGCCAGCCCTTCCAGCATTCGTAGCCTG
AAGTCGGACATGATGTCACACCTGGTGAAGATCCCTGGCATCATCATCGCGGCCTCTGCG
GTCCGTGCCAAGGCCACCCGCATCTCTATCCAGTGCCGCAGCTGCCGCAACACCCTCACC
AACATTGCCATGCGCCCTGGCCTCGAGGGCTATGCCCTGCCCAGGAAGTGCAACACAGAT
CAGGCTGGGCGCCCCAAATGCCCATTGGACCCGTACTTCATCATGCCCGACAAATGCAAA
TGCGTGGACTTCCAGACCCTGAAGCTGCAGGAGCTGCCTGATGCAGTCCCCCACGGGGAG
ATGCCCAGACACATGCAGCTCTACTGCGACAGGTACCTGTGTGACAAGGTCGTCCCTGGG
AACAGGGTTACCATCATGGGCATCTACTCCATCAAGAAGTTTGGCCTGACCACCAGCAGG
GGCCGTGACAGGGTGGGCGTGGGCATCCGAAGCTCCTACATCCGTGTCCTGGGCATCCAG
GTGGACACAGATGGCTCTGGCCGCAGCTTTGCTGGGGCCGTGAGCCCCCAGGAGGAGGAG
GAGTTCCGTCGCCTGGCTGCCCTCCCAAATGTCTATGAGGTCATCTCCAAGAGCATCGCC
CCCTCCATCTTTGGGGGCACAGACATGAAGAAGGCCATTGCCTGCCTGCTCTTTGGGGGC
TCCCGAAAGAGGCTCCCTGATGGACTTACTCGCCGAGGAGACATCAACCTGCTGATGCTA
GGGGACCCTGGGACAGCCAAGTCCCAGCTTCTGAAGTTTGTGGAGAAGTGTTCTCCCATT
GGGGTATACACGTCTGGGAAAGGCAGCAGCGCAGCTGGACTGACAGCCTCGGTGATGAGG
GACCCTTCGTCCCGGAATTTCATCATGGAGGGCGGAGCCATGGTCCTGGCCGATGGTGGG
GTCGTCTGTATTGACGAGTTTGACAAGATGCGAGAAGATGACCGTGTGGCAATCCACGAA
GCCATGGAGCAGCAGACCATCTCTATCGCCAAGGCTGGGATCACCACCACCCTGAACTCC
CGCTGCTCCGTCCTGGCTGCTGCCAACTCAGTGTTCGGCCGCTGGGATGAGACGAAGGGG
GAGGACAACATTGACTTCATGCCCACCATCTTGTCGCGCTTCGACATGATCTTCATCGTC
AAGGATGAGCACAATGAGGAGAGGGATGTGATGCTGGCCAAGCATGTCATCACTCTGCAC
GTGAGCGCACTGACACAGACACAGGCTGTGGAGGGCGAGATTGACCTGGCCAAGCTGAAG
AAGTTTATTGCCTACTGCCGAGTGAAATGTGGCCCCCGGCTGTCAGCAGAGGCTGCAGAG
AAACTGAAGAACCGCTACATCATCATGCGGAGCGGGGCCCGTCAGCACGAGAGGGACAGT
GACCGCCGCTCCAGCATCCCCATCACTGTGCGGCAGCTGGAGGCCATTGTGCGCATCGCG
GAAGCCCTCAGCAAGATGAAGCTGCAGCCCTTCGCCACAGAGGCAGATGTGGAGGAGGCC
CTGCGGCTCTTCCAAGTGTCCACGTTGGATGCTGCCTTGTCCGGTACCCTGTCAGGGGTG
GAGGGCTTCACCAGCCAGGAGGACCAGGAGATGCTGAGCCGCATCGAGAAGCAGCTCAAG
CGCCGCTTTGCCATTGGCTCCCAGGTGTCTGAGCACAGCATCATCAAGGACTTCACCAAG
CAGAAATACCCGGAGCACGCCATCCACAAGGTGCTGCAGCTCATGCTGCGGCGCGGCGAG
ATCCAGCATCGCATGCAGCGCAAGGTTCTCTACCGCCTCAAG

Protein sequence predicted based on nucleotide sequence

MSGFDDPGIFYSDSFGGDAQADEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELK
RHYNLGEYWIEVEMEDLASFDEDLADYLYKQPAEHLQLLEEAAKEVADEVTRPRPSGEEV
LQDIQVMLKSDASPSSIRSLKSDMMSHLVKIPGIIIAASAVRAKATRISIQCRSCRNTLT
NIAMRPGLEGYALPRKCNTDQAGRPKCPLDPYFIMPDKCKCVDFQTLKLQELPDAVPHGE
MPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIKKFGLTTSRGRDRVGVGIRSSYIRVLGIQ
VDTDGSGRSFAGAVSPQEEEEFRRLAALPNVYEVISKSIAPSIFGGTDMKKAIACLLFGG
SRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMR
DPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNS
RCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLH
VSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDS
DRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGV
EGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGE
IQHRMQRKVLYRLK

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable