Gene:
Gene Symbol:
SEMA3B
Gene Name:
sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B
Original Clone ID:
ccsbBroadEn_01837
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC013975.2,BC024220.2
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505565
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2245nts
Open reading frame: 1 to 2245
ATGGGGCGGGCCGGGGCTGCCGCCGTGATCCCGGGCCTGGCCCTGCTCTGGGCAGTGGGG
CTGGGGAGTGCCGCCCCCAGCCCCCCACGCCTTCGGCTCTCCTTCCAAGAGCTCCAGGCC
TGGCATGGTCTCCAGACTTTCAGCCTGGAGCGAACCTGCTGCTACCAGGCCTTGCTGGTG
GATGAGGAGCGTGGACGCCTGTTTGTGGGTGCCGAGAACCATGTGGCCTCCCTCAACCTG
GACAACATCAGCAAGCGGGCCAAGAAGCTGGCCTGGCCGGCCCCTGTGGAATGGCGAGAG
GAGTGCAACTGGGCAGGGAAGGACATTGGTACTGAGTGCATGAACTTCGTGAAGTTGCTG
CATGCCTACAACCGCACCCATTTGCTGGCCTGTGGCACGGGAGCCTTCCACCCAACCTGT
GCCTTTGTGGAAGTGGGCCACCGGGCAGAGGAGCCCGTCCTCCGGCTGGACCCAGGAAGG
ATAGAGGATGGCAAGGGGAAGAGTCCTTATGACCCCAGGCATCGGGCTGCCTCCGTGCTG
GTGGGGGAGGAGCTATACTCAGGGGTGGCAGCAGACCTCATGGGACGAGACTTTACCATC
TTTCGCAGCCTAGGGCAACGTCCAAGTCTCCGAACAGAGCCACACGACTCCCGCTGGCTC
AATGAGCCCAAGTTTGTCAAGGTATTTTGGATCCCGGAGAGCGAGAACCCAGACGACGAC
AAAATCTACTTCTTCTTTCGTGAGACGGCGGTAGAGGCGGCGCCGGCACTGGGACGCCTG
TCCGTGTCCCGCGTTGGCCAGATCTGCCGGAACGACGTGGGCGGCCAGCGCAGCCTGGTC
AACAAGTGGACGACGTTCCTGAAGGCGCGGCTGGTGTGCTCGGTGCCCGGCGTCGAGGGC
GACACCCACTTCGATCAGCTCCAGGATGTGTTTCTGTTGTCCTCGCGGGACCACCGGACC
CCGCTGCTCTATGCCGTCTTCTCCACGTCCAGCATCTTCCAGGGCTCTGCGGTGTGCGTG
TACAGCATGAACGACGTGCGCCGGGCCTTCTTGGGACCCTTTGCACACAAGGAGGGGCCC
ATGCACCAGTGGGTGTCATACCAGGGTCGCGTCCCCTACCCGCGGCCAGGCATGTGCCCC
AGCAAGACCTTTGGCACCTTCAGTTCCACCAAGGACTTCCCAGACGATGTCATCCAGTTT
GCGCGGAACCACCCCCTCATGTACAACTCTGTCCTGCCCACTGGGGGGCGCCCTCTTTTC
CTACAAGTTGGAGCCAATTACACCTTCACTCAAATTGCCGCGGACCGGGTTGCAGCCGCT
GACGGACACTATGACGTCCTCTTCATTGGCACAGACGTTGGCACGGTGCTGAAGGTGATC
TCGGTCCCCAAGGGCAGTAGGCCCAGCGCAGAGGGGCTGCTCCTGGAGGAGCTGCACGTG
TTTGAGGACTCGGCCGCTGTCACCAGCATGCAAATTTCTTCCAAGAGGCACCAGCTGTAC
GTAGCCTCGCGGAGCGCGGTGGCCCAGATCGCGTTGCACCGCTGCGCTGCCCACGGCCGC
GTCTGCACCGAATGCTGTCTGGCGCGTGACCCCTACTGCGCCTGGGACGGGGTCGCGTGC
ACGCGCTTCCAGCCCAGTGCCAAGAGGCGGTTCCGGCGGCAAGACGTAAGGAATGGCGAC
CCCAGCACGTTGTGCTCCGGAGACTCGTCTCGTCCCGCGCTGCTGGAACACAAGGTGTTC
GGCGTGGAGGGCAGCAGCGCCTTTCTGGAGTGTGAGCCCCGCTCGCTGCAGGCGCGCGTG
GAGTGGACTTTCCAGCGCGCAGGGGTGACAGCCCACACCCAGGTGCTGGCAGAGGAGCGC
ACCGAGCGCACCGCCCGGGGACTACTGCTGCGCAGGCTGCGGCGCCGGGACTCGGGCGTG
TACTTGTGCGCCGCCGTCGAGCAGGGCTTTACGCAACCGCTGCGTCGCCTGTCGCTGCAC
GTGTTGAGTGCTACGCAGGCCGAACGACTGGCGCGGGCCGAGGAGGCTGCGCCCGCCGCG
CCGCCGGGCCCCAAACTCTGGTACCGGGACTTTCTGCAGCTGGTGGAGCCGGGCGGAGGT
GGCAGCGCGAACTCCCTGCGCATGTGCCGCCCGCAGCCTGCGCTGCAGTCACTGCCCCTG
GAGTCGCGGAGAAAGGGCCGTAACCGGAGGACCCACGCCCCTGAGCCTCGCGCTGAGCGG
GGGCCGCGCAGCGCAACGCACTGG
Protein sequence predicted based on nucleotide sequence
MGRAGAAAVIPGLALLWAVGLGSAAPSPPRLRLSFQELQAWHGLQTFSLERTCCYQALLV DEERGRLFVGAENHVASLNLDNISKRAKKLAWPAPVEWREECNWAGKDIGTECMNFVKLL HAYNRTHLLACGTGAFHPTCAFVEVGHRAEEPVLRLDPGRIEDGKGKSPYDPRHRAASVL VGEELYSGVAADLMGRDFTIFRSLGQRPSLRTEPHDSRWLNEPKFVKVFWIPESENPDDD KIYFFFRETAVEAAPALGRLSVSRVGQICRNDVGGQRSLVNKWTTFLKARLVCSVPGVEG DTHFDQLQDVFLLSSRDHRTPLLYAVFSTSSIFQGSAVCVYSMNDVRRAFLGPFAHKEGP MHQWVSYQGRVPYPRPGMCPSKTFGTFSSTKDFPDDVIQFARNHPLMYNSVLPTGGRPLF LQVGANYTFTQIAADRVAAADGHYDVLFIGTDVGTVLKVISVPKGSRPSAEGLLLEELHV FEDSAAVTSMQISSKRHQLYVASRSAVAQIALHRCAAHGRVCTECCLARDPYCAWDGVAC TRFQPSAKRRFRRQDVRNGDPSTLCSGDSSRPALLEHKVFGVEGSSAFLECEPRSLQARV EWTFQRAGVTAHTQVLAEERTERTARGLLLRRLRRRDSGVYLCAAVEQGFTQPLRRLSLH VLSATQAERLARAEEAAPAAPPGPKLWYRDFLQLVEPGGGGSANSLRMCRPQPALQSLPL ESRRKGRNRRTHAPEPRAERGPRSATHW
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
