TAF1C (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  TAF1C
Gene Name:  TATA box binding protein (TBP)-associated factor, RNA polymerase I, C, 110kDa
Original Clone ID:
ccsbBroadEn_07336
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC028131.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505658
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2326nts
Open reading frame: 1 to 2326

ATGCTGCCTCCCCTCATCGATCCCTGGGACCCTGGCCTGACTGCCCGGGACCTGCTTTTC
CGCGGAGGGTACCGGTATCGGAAGCGGCCCCGAGTCGTGCTGGATGTGACTGAGCAGATC
AGCCGGTTCCTCTTGGATCATGGAGACGTAGCCTTTGCGCCCCTGGGGAAGCTGATGCTG
GAGAATTTCAAGCTGGAGGGAGCGGGGAGCCGCACTAAGAAGAAGACAGTGGTCAGTGTG
AAGAAGCTGCTCCAGGACCTCGGTGGACACCAGCCCTGGGGGTGTCCCTGGGCTTACCTC
AGCAACCGACAGCGCCGCTTCTCTATCCTCGGGGGCCCCATCCTGGGCACGTCGGTGGCG
AGCCACTTGGCAGAGCTGCTGCACGAGGAGCTGGTGCTGCGGTGGGAGCAGCTGCTTCTG
GATGAGGCCTGCACTGGGGGCGCGCTGGCCTGGGTTCCTGGAAGGACACCCCAGTTCGGG
CAGCTGGTCTACCCTGCTGGAGGCGCCCAGGACAGGCTGCATTTCCAAGAGGTCGTTCTG
ACCCCAGGTGACAATCCCCAATTCCTTGGGAAACCTGGACGCATCCAGCTCCAGGGACCT
GTCCGGCAAGTGGTGACATGCACCGTCCAGGGAGAAACTCTGCTGGCCGTCCGCTCTGAC
TACCACTGTGCCGTGTGGAAGTTTGGTAAACAGTGGCAGCCAACCCTTCTGCAGGCGATG
CAGGTGGAGAAAGGGGCCACGGGGATCAGCCTCAGCCCTCACCTGCCCGGGGAGCTGGCC
ATCTGCAGCCGCTCGGGAGCCGTCTGCCTGTGGAGCCCTGAGGATGGGCTGCGGCAAATC
TACAGGGACCCTGAGACCCTCGTGTTCCGGGACTCCTCTTCGTGGCGTTGGGCAGACTTC
ACTGCGCACCCTCGGGTGCTGACCGTGGGTGACCGCACCGGAGTGAAGATGCTGGACACT
CAGGGCCCGCCGGGCTGTGGTCTGTTGCTTTTTCGTTTGGGGGCAGAGGCTTCGTGCCAG
AAAGGGGAACGTGTCCTGCTTACCCAGTACCTGGGGCACTCCAGCCCCAAATGCCTCCCC
CCTACTCTTCATCTCGTCTGTACCCAGTTCTCTCTCTACCTAGTGGACGAGCGCCTTCCC
CTGGTGCCGATGCTGAAGTGGAACCATGGCCTCCCCTCCCCGCTCCTGCTGGCCCGACTG
CTGCCTCCGCCCCGGCCCAGCTGCGTGCAGCCCCTGCTCCTCGGAGGCCAGGGTGGGCAG
CTGCAGCTGCTGCACCTGGCAGAAGGGGCGTCGGTGCCCCGCCTGGCAGGCCCCCCCCAG
TCTCTTCCTTCCAGGATCGACTCCCTCCCTGCATTTCCTCTGCTGGAGCCTAAGATCCAG
TGGCGGCTGCAGGAGCGCCTGAAAGCACCGACCATAGGTCTGGCTGCCGTCGTCCCGCCC
ATGCCCTCAGCGCCCACACCAGGCCTGGTGCTCTTCCAGCTCTCGGCGGCGGGAGATGTC
TTCTACCAGCAGCTCCGCCCCCAGGTGGACTCCAGCCTCCGCAGAGATGCTGGGCCTCCT
GGCGACACCCAACCTGACTGCCATGCCCCCACAGCTTCCTGGACCTCCCAGGACACTGCC
GGCTGCAGCCAGTGGCTGAAGGCCCTGCTAAAAGTGCCCCTGGCTCCTCCTGTGTGGACA
GCACCCACCTTCACCCACCGCCAGATGCTGGGCAGCACAGAGCTGCGGAGGGAGGAAGAG
GAAGGGCAGCGGCTGGGTGTACTCCGCAAGGCCATGGCCCGAGGGCAGCTCCTGCTGCAG
AGAGACCTGGGCTCCCTCCCTGCGGCAGAGCCACCCCCTGCACCCGAGTCAGGCCTAGAG
GACAAGCTCAGTGAGCGCCTGGGGGAAGCCTGGGCAGGCCGAGGGGCTGCCTGGTGGGAG
AGGCAGCAGGGCAGGACCTCGGAGCCCGGGAGACAGACCAGGCGGCCCAAGCGCCGGACC
CAGCTGTCCAGCAGCTTTTCGCTCAGTGGCCATGTGGATCCCTCAGAGGACACCAGCTCC
CCTCATAGCCCTGAGTGGCCACCTGCTGATGCTCTGCCCCTGCCCCCCACGACCCCGCCC
TCCCAGGAGTTGACTCCGGATGCATGCGCCCAGGGCGTCCCATCAGAGCAGCGGCAGATG
CTCCGTGACTACATGGCCAAGCTACCACCCCAGAGGGACACCCCAGGCTGTGCCACCACA
CCTCCCCACTCCCAGGCCTCCAGCGTCCGGGCCACTCGCTCCCAGCAGCACACACCCGTC
CTCTCTAGCTCTCAGCCCCTCCGTAAGAAGCCTCGAATGGGCTTC

Protein sequence predicted based on nucleotide sequence

MLPPLIDPWDPGLTARDLLFRGGYRYRKRPRVVLDVTEQISRFLLDHGDVAFAPLGKLML
ENFKLEGAGSRTKKKTVVSVKKLLQDLGGHQPWGCPWAYLSNRQRRFSILGGPILGTSVA
SHLAELLHEELVLRWEQLLLDEACTGGALAWVPGRTPQFGQLVYPAGGAQDRLHFQEVVL
TPGDNPQFLGKPGRIQLQGPVRQVVTCTVQGETLLAVRSDYHCAVWKFGKQWQPTLLQAM
QVEKGATGISLSPHLPGELAICSRSGAVCLWSPEDGLRQIYRDPETLVFRDSSSWRWADF
TAHPRVLTVGDRTGVKMLDTQGPPGCGLLLFRLGAEASCQKGERVLLTQYLGHSSPKCLP
PTLHLVCTQFSLYLVDERLPLVPMLKWNHGLPSPLLLARLLPPPRPSCVQPLLLGGQGGQ
LQLLHLAEGASVPRLAGPPQSLPSRIDSLPAFPLLEPKIQWRLQERLKAPTIGLAAVVPP
MPSAPTPGLVLFQLSAAGDVFYQQLRPQVDSSLRRDAGPPGDTQPDCHAPTASWTSQDTA
GCSQWLKALLKVPLAPPVWTAPTFTHRQMLGSTELRREEEEGQRLGVLRKAMARGQLLLQ
RDLGSLPAAEPPPAPESGLEDKLSERLGEAWAGRGAAWWERQQGRTSEPGRQTRRPKRRT
QLSSSFSLSGHVDPSEDTSSPHSPEWPPADALPLPPTTPPSQELTPDACAQGVPSEQRQM
LRDYMAKLPPQRDTPGCATTPPHSQASSVRATRSQQHTPVLSSSQPLRKKPRMGF

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable