PGBD1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PGBD1
Gene Name:  piggyBac transposable element derived 1
Original Clone ID:
ccsbBroadEn_04399
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC128585.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505724
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2428nts
Open reading frame: 1 to 2428

ATGTATGAAGCTTTGCCAGGCCCTGCTCCTGAAAATGAAGATGGCCTTGTGAAAGTGAAG
GAGGAAGATCCCACCTGGGAGCAGGTGTGCAACTCACAGGAGGGCAGCTCCCACACTCAG
GAGATTTGCCGCCTGCGCTTTCGGCACTTCTGCTACCAGGAGGCTCACGGACCCCAGGAA
GCTCTGGCCCAACTCCGAGAACTTTGTCATCAATGGCTGAGACCGGAGATGCACACCAAG
GAACAGATAATGGAACTGCTGGTGCTGGAGCAGTTCCTGACCATCCTGCCCAAGGAGCTC
CAGCCCTGTGTGAAGACATATCCTCTGGAGAGTGGAGAGGAGGCAGTGACAGTGCTGGAG
AATCTAGAGACAGGAAGTGGAGACACAGGACAACAGGCCTCTGTCTATATTCAGGGACAG
GACATGCACCCAATGGTGGCAGAATATCAAGGAGTCTCTTTGGAGTGTCAGAGCCTCCAG
CTCCTGCCTGGGATAACCACCCTGAAGTGTGAACCTCCACAGCGTCCTCAAGGGAACCCC
CAAGAAGTGAGTGGGCCTGTTCCCCACGGATCAGCTCATCTCCAGGAAAAAAACCCCAGA
GACAAGGCTGTAGTGCCTGTGTTTAACCCAGTCAGGTCCCAGACATTGGTGAAGACTGAG
GAAGAAACAGCCCAGGCCGTTGCTGCAGAGAAGTGGTCACATCTGAGTCTGACTCGGAGG
AACCTCTGTGGGAACTCAGCTCAGGAGACAGTTATGAGCCTCAGTCCGATGACTGAAGAA
ATTGTAACTAAAGATAGATTGTTTAAAGCAAAGCAAGAAACTTCTGAAGAAATGGAACAA
AGTGGAGAAGCCTCAGGAAAGCCCAACAGAGAGTGTGCACCCCAGATTCCTTGTAGTACT
CCTATTGCTACTGAAAGGACAGTTGCACATTTGAACACTCTGAAGGACCGTCACCCAGGT
GATTTGTGGGCCCGCATGCACATCTCATCCCTGGAATATGCTGCAGGAGACATTACCCGA
AAAGGGAGAAAAAAAGACAAAGCTCGAGTGAGTGAACTGCTCCAAGGCCTCTCATTCTCT
GGTGACTCAGATGTGGAAAAAGATAATGAGCCTGAGATCCAGCCTGCTCAAAAGAAGTTA
AAGGTATCATGTTTCCCAGAAAAGAGTTGGACCAAAAGAGACATTAAACCCAATTTTCCA
AGCTGGTCAGCACTGGATTCTGGACTTTTGAATCTCAAGAGCGAAAAGTTGAACCCAGTA
GAGCTTTTTGAATTATTTTTTGATGATGAAACATTCAACTTAATTGTCAATGAAACCAAT
AATTATGCTTCTCAGAAAAATGTCAGCTTGGAAGTCACAGTTCAGGAAATGAGGTGTGTG
TTTGGTGTCTTACTTTTGAGTGGATTTATGAGGCATCCTAGAAGGGAAATGTATTGGGAA
GTCTCTGACACCGATCAGAACCTGGTTAGAGATGCAATCAGAAGGGACAGATTTGAATTG
ATTTTCTCAAACCTGCACTTTGCAGATAATGGCCACCTAGATCAAAAAGATAAGTTTACA
AAGTTGAGACCTCTCATAAAACAAATGAATAAAAATTTCCTCTTGTATGCTCCCCTGGAA
GAATACTATTGCTTTGATAAGTCAATGTGTGAATGCTTTGATAGTGACCAATTCCTGAAT
GGAAAGCCTATTAGAATTGGCTATAAAATTTGGTGTGGTACAACCACACAGGGTTATCTG
GTTTGGTTTGAACCCTATCAAGAAGAATCAACTATGAAGGTAGATGAGGATCCTGATCTT
GGGTTAGGTGGAAATCTAGTGATGAACTTCGCTGATGTTCTTTTAGAGAGAGGTCAGTAT
CCCTATCACCTGTGTTTTGATAGCTTCTTTACAAGTGTCAAATTGTTGTCAGCCTTGAAA
AAGAAGGGGGTGAGGGCAACAGGAACAATTCGTGAGAACAGGACCGAAAAATGTCCCCTT
ATGAATGTAGAACATATGAAAAAAATGAAGAGAGGGTATTTTGATTTCCGAATAGAAGAA
AACAATGAGATAATTTTGTGTCGTTGGTATGGGGATGGCATTATCAGTCTGTGCTCCAAT
GCTGTGGGCATAGAACCAGTCAATGAGGTAAGCTGTTGTGATGCTGATAACGAAGAAATC
CCTCAGATAAGTCAACCATCCATAGTAAAAGTGTATGATGAATGCAAGGAAGGTGTAGCT
AAAATGGATCAAATTATTTCGAAATACAGGGTGAGGATAAGAAGCAAGAAATGGTACTCA
ATTTTGGTGAGCTACATGATTGATGTAGCCATGAACAATGCATGGCAACTACACAGAGCC
TGTAACCCAGGTGCTTCTCTAGACCCCTTGGATTTTCGGAGATTTGTTGCACATTTCTAC
TTGGAACACAATGCTCATCTGTCAGAT

Protein sequence predicted based on nucleotide sequence

MYEALPGPAPENEDGLVKVKEEDPTWEQVCNSQEGSSHTQEICRLRFRHFCYQEAHGPQE
ALAQLRELCHQWLRPEMHTKEQIMELLVLEQFLTILPKELQPCVKTYPLESGEEAVTVLE
NLETGSGDTGQQASVYIQGQDMHPMVAEYQGVSLECQSLQLLPGITTLKCEPPQRPQGNP
QEVSGPVPHGSAHLQEKNPRDKAVVPVFNPVRSQTLVKTEEETAQAVAAEKWSHLSLTRR
NLCGNSAQETVMSLSPMTEEIVTKDRLFKAKQETSEEMEQSGEASGKPNRECAPQIPCST
PIATERTVAHLNTLKDRHPGDLWARMHISSLEYAAGDITRKGRKKDKARVSELLQGLSFS
GDSDVEKDNEPEIQPAQKKLKVSCFPEKSWTKRDIKPNFPSWSALDSGLLNLKSEKLNPV
ELFELFFDDETFNLIVNETNNYASQKNVSLEVTVQEMRCVFGVLLLSGFMRHPRREMYWE
VSDTDQNLVRDAIRRDRFELIFSNLHFADNGHLDQKDKFTKLRPLIKQMNKNFLLYAPLE
EYYCFDKSMCECFDSDQFLNGKPIRIGYKIWCGTTTQGYLVWFEPYQEESTMKVDEDPDL
GLGGNLVMNFADVLLERGQYPYHLCFDSFFTSVKLLSALKKKGVRATGTIRENRTEKCPL
MNVEHMKKMKRGYFDFRIEENNEIILCRWYGDGIISLCSNAVGIEPVNEVSCCDADNEEI
PQISQPSIVKVYDECKEGVAKMDQIISKYRVRIRSKKWYSILVSYMIDVAMNNAWQLHRA
CNPGASLDPLDFRRFVAHFYLEHNAHLSD

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable