PIWIL2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PIWIL2
Gene Name:  piwi-like RNA-mediated gene silencing 2
Original Clone ID:
ccsbBroadEn_03529
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC025995.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505878
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2920nts
Open reading frame: 1 to 2920

ATGGATCCTTTCCGACCATCGTTCAGGGGCCAGTCTCCTATCCACCCATCCCAGTGCCAG
GCTGTACGGATGCCAGGCTGTTGGCCACAAGCTTCTAAACCTTTGGACCCAGCTCTGGGC
AGGGGAGCACCTGCAGGCAGAGGCCATGTATTTGGAAAGCCAGAGGAACCAAGCACACAG
AGGGGGCCAGCACAAAGGGAGTCTGTGGGTTTGGTCTCCATGTTCCGAGGCCTGGGCATT
GAAACAGTTTCTAAGACCCCTCTGAAACGGGAAATGCTTCCATCAGGTAGAGGCATTTTA
GGTCGAGGCTTGTCTGCTAATCTGGTACGCAAGGACAGGGAGGAACTCTCTCCCACTTTT
TGGGATCCAAAAGTGTTGGCGGCTGGGGACAGCAAGATGGCAGAGACCTCCGTTGGTTGG
AGTAGGACGCTTGGAAGAGGGAGTTCAGATGCGTCTTTATTACCACTGGGAAGAGCAGCA
GGTGGTATCAGCAGAGAAGTGGACAAGCCTCCCTGTACCTTCAGCACACCGTCCCGGGGT
CCCCCGCAGCTGTCATCACCACCAGCTCTGCCCCAGTCTCCCCTGCACTCTCCAGATCGC
CCTCTGGTCCTGACTGTGGAACACAAGGAAAAAGAGCTTATTGTGAAGCAAGGATCAAAA
GGAACACCTCAGTCTTTGGGACTGAACCTCGTCAAAATACAGTGTCATAATGAAGCAGTT
TATCAATATCATGTGACTTTCAGCCCCAATGTGGAGTGCAAAAGCATGAGGTTCGGCATG
TTGAAGGACCATCAAGCTGTCACCGGCAACGTCACTGCGTTTGATGGATCTATTCTCTAT
CTGCCTGTTAAGCTTCAACAAGTTCTTGAGTTAAAAAGTCAAAGGAAAACAGACAGTGCT
GAAATCAGCATTAAGATTCAGATGACAAAGATCCTGGAGCCCTGCTCTGACCTGTGCATT
CCCTTCTACAATGTTGTTTTCCGTCGGGTAATGAAACTTTTAGATATGAAGCTTGTGGGG
AGAAACTTTTATGACCCTACAAGTGCTATGGTACTACAGCAACACAGATTGCAGATCTGG
CCAGGCTATGCAGCTAGCATCCGAAGGACAGATGGAGGGCTCTTCCTGCTAGCTGATGTC
TCCCATAAGGTCATTCGGAATGACTGTGTGCTGGATGTCATGCATGCCATTTATCAGCAG
AATAAAGAACACTTCCAGGATGAGTGTACTAAGCTTCTGGTTGGCAATATTGTTATCACC
CGATATAACAATCGTACCTATCGTATTGATGATGTGGATTGGAATAAGACTCCAAAGGAT
AGCTTCACGATGTCTGATGGGAAAGAGATCACATTCTTGGAATACTACAGCAAAAATTAT
GGGATCACAGTTAAGGAAGAGGACCAGCCATTGCTGATTCACAGGCCCAGTGAGAGACAG
GATAATCATGGGATGCTGCTAAAAGGGGAAATCCTGCTGCTGCCTGAGCTTTCTTTTATG
ACCGGAATCCCAGAGAAGATGAAGAAGGACTTCAGAGCCATGAAGGATTTGGCTCAGCAA
ATCAATCTGAGCCCCAAGCAACACCATAGTGCTTTGGAATGCTTGCTGCAAAGAATTGCA
AAGAACGAGGCAGCCACCAATGAACTGATGCGTTGGGGGCTCCGTCTGCAAAAGGATGTA
CATAAGATTGAAGGACGTGTTCTGCCAATGGAAAGAATTAACTTAAAAAATACTTCGTTT
ATCACATCTCAGGAACTAAACTGGGTTAAGGAAGTAACCAGAGACCCTTCCATCTTGACT
ATCCCCATGCATTTCTGGGCACTTTTTTACCCAAAGAGAGCAATGGACCAGGCTCGAGAA
CTGGTCAACATGTTGGAGAAGATAGCCGGCCCCATTGGCATGCGTATGAGCCCACCGGCC
TGGGTTGAACTAAAGGATGACCGAATAGAGACTTATGTCAGAACCATTCAATCCACGTTA
GGAGCTGAGGGGAAGATACAGATGGTTGTTTGCATCATCATGGGCCCACGTGATGATCTC
TATGGGGCCATCAAGAAGCTGTGCTGTGTGCAGTCCCCAGTGCCCTCCCAGGTTGTCAAT
GTTCGAACCATTGGTCAGCCCACCAGGCTTCGGAGTGTGGCCCAGAAGATTTTACTTCAG
ATTAACTGTAAATTGGGTGGTGAGCTCTGGGGAGTGGATATTCCTCTGAAACAGTTAATG
GTGATCGGGATGGATGTTTACCATGACCCCAGTAGAGGCATGCGCTCCGTGGTTGGCTTC
GTGGCAAGCATCAATCTCACCCTCACAAAATGGTATTCCCGGGTGGTGTTCCAGATGCCG
CATCAGGAGATTGTGGACAGCCTGAAGCTATGCCTCGTGGGCTCCTTAAAAAAGTTTTAT
GAGGTGAACCACTGTCTACCAGAGAAGATTGTGGTGTACCGTGATGGAGTGTCTGATGGC
CAACTGAAGACAGTTGCCAACTATGAGATTCCTCAACTACAGAAGTGTTTTGAAGCTTTT
GAGAATTATCAGCCCAAGATGGTGGTGTTTGTAGTTCAGAAGAAAATCAGTACTAATCTA
TATCTGGCTGCTCCTCAGAACTTTGTAACTCCCACTCCTGGAACTGTGGTAGATCATACA
ATAACAAGCTGTGAGTGGGTGGATTTCTATCTTCTTGCCCATCATGTACGGCAGGGCTGT
GGCATTCCTACGCATTATGTCTGTGTTCTCAACACCGCAAACCTGAGCCCTGATCATATG
CAGAGGCTGACTTTCAAACTGTGCCACATGTACTGGAATTGGCCTGGCACCATCAGAGTT
CCAGCTCCTTGCAAGTATGCCCACAAGCTAGCTTTCCTGTCAGGACACATCTTGCATCAT
GAACCAGCCATCCAGCTGTGCGAGAACCTGTTCTTCCTG

Protein sequence predicted based on nucleotide sequence

MDPFRPSFRGQSPIHPSQCQAVRMPGCWPQASKPLDPALGRGAPAGRGHVFGKPEEPSTQ
RGPAQRESVGLVSMFRGLGIETVSKTPLKREMLPSGRGILGRGLSANLVRKDREELSPTF
WDPKVLAAGDSKMAETSVGWSRTLGRGSSDASLLPLGRAAGGISREVDKPPCTFSTPSRG
PPQLSSPPALPQSPLHSPDRPLVLTVEHKEKELIVKQGSKGTPQSLGLNLVKIQCHNEAV
YQYHVTFSPNVECKSMRFGMLKDHQAVTGNVTAFDGSILYLPVKLQQVLELKSQRKTDSA
EISIKIQMTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAMVLQQHRLQIW
PGYAASIRRTDGGLFLLADVSHKVIRNDCVLDVMHAIYQQNKEHFQDECTKLLVGNIVIT
RYNNRTYRIDDVDWNKTPKDSFTMSDGKEITFLEYYSKNYGITVKEEDQPLLIHRPSERQ
DNHGMLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLAQQINLSPKQHHSALECLLQRIA
KNEAATNELMRWGLRLQKDVHKIEGRVLPMERINLKNTSFITSQELNWVKEVTRDPSILT
IPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRMSPPAWVELKDDRIETYVRTIQSTL
GAEGKIQMVVCIIMGPRDDLYGAIKKLCCVQSPVPSQVVNVRTIGQPTRLRSVAQKILLQ
INCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMP
HQEIVDSLKLCLVGSLKKFYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAF
ENYQPKMVVFVVQKKISTNLYLAAPQNFVTPTPGTVVDHTITSCEWVDFYLLAHHVRQGC
GIPTHYVCVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGHILHH
EPAIQLCENLFFL

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable