EIF3C (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  EIF3CL
Gene Name:  eukaryotic translation initiation factor 3, subunit C-like
Original Clone ID:
ccsbBroadEn_01982
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC001571.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505903
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2740nts
Open reading frame: 1 to 2740

ATGTCGCGGTTTTTCACCACCGGTTCGGACAGCGAGTCCGAGTCGTCCTTGTCCGGGGAG
GAGCTCGTCACCAAACCTGTCGGAGGCAACTATGGCAAACAGCCATTGTTGCTGAGCGAG
GATGAAGAAGATACCAAGAGAGTTGTCCGCAGTGCCAAGGACAAGAGGTTTGAGGAGCTG
ACCAACCTTATCCGGACCATCCGTAATGCCATGAAGATTCGTGATGTCACCAAGTGCCTG
GAAGAGTTTGAGCTCCTGGGAAAAGCATATGGGAAGGCCAAAAGCATTGTGGACAAAGAA
GGTGTCCCCCGGTTCTATATCCGCATCCTGGCTGACCTAGAGGACTATCTTAATGAGCTT
TGGGAAGATAAGGAAGGGAAGAAGAAGATGAACAAGAACAATGCCAAGGCTCTGAGCACC
TTGCGTCAGAAGATCCGAAAATACAACCGTGATTTCGAGTCCCATATCACAAGCTACAAG
CAGAACCCCGAGCAGTCTGCGGATGAAGATGCTGAGAAAAATGAGGAGGATTCAGAAGGC
TCTTCAGATGAGGATGAGGATGAGGACGGAGTCAGTGCTGCAACTTTCTTGAAGAAGAAA
TCAGAAGCTCCTTCTGGGGAGAGTCGCAAGTTCCTCAAAAAGATGGATGATGAAGATGAG
GACTCAGAAGATTCCGAAGATGATGAAGACTGGGACACAGGTTCCACATCTTCCGATTCC
GACTCAGAGGAGGAAGAAGGGAAACAAACCGCGCTGGCCTCAAGATTTCTTAAAAAGGCA
CCCACCACAGATGAGGACAAGAAGGCAGCCGAGAAGAAACGGGAGGACAAAGCTAAGAAG
AAGCACGACAGGAAATCCAAGCGCCTGGATGAGGAGGAGGAGGACAATGAAGGCGGGGAG
TGGGAAAGGGTCCGGGGCGGAGTGCCGTTGGTTAAGGAGAAGCCAAAAATGTTTGCCAAG
GGAACTGAGATCACCCATGCTGTTGTTATCAAGAAACTGAATGAGATCCTACAGGCACGA
GGCAAGAAGGGAACTGATCGTGCTGCCCAGATTGAGCTGCTGCAACTGCTGGTTCAGATT
GCAGCGGAAAACAACCTGGGAGAGGGCGTCATTGTCAAGATCAAGTTCAATATCATCGCC
TCTCTCTATGACTACAACCCCAACCTGGCAACCTACATGAAGCCAGAGATGTGGGGGAAG
TGCCTGGACTGCATCAATGAGCTGATGGATATCCTGTTTGCAAATCCCAACATTTTTGTT
GGAGAGAATATTCTGGAAGAGAGTGAGAACCTGCACAACGCTGACCAGCCACTGCGTGTC
CGTGGCTGCATCCTAACTCTGGTGGAACGAATGGATGAAGAATTTACCAAAATAATGCAA
AATACTGACCCTCACTCCCAAGAGTACGTGGAGCACTTGAAGGATGAGGCCCAGGTGTGT
GCCATCATCGAGCGTGTGCAGCGCTACCTGGAGGAGAAGGGCACTACCGAGGAGGTCTGC
CGCATCTACCTGCTGCGCATCCTGCACACCTACTACAAGTTTGATTACAAGGCCCATCAG
CGACAGCTGACCCCGCCTGAGGGCTCCTCAAAGTCTGAGCAAGACCAGGCAGAAAATGAG
GGCGAGGACTCGGCTGTGTTGATGGAGAGACTGTGCAAGTACATCTACGCCAAGGACCGC
ACAGACCGGATCCGCACATGTGCCATCCTCTGCCACATCTACCACCATGCTCTGCACTCG
CGCTGGTACCAGGCCCGCGACCTCATGCTCATGAGCCACTTGCAGGACAACATTCAGCAT
GCAGACCCGCCAGTGCAGATCCTTTACAACCGCACCATGGTGCAGCTGGGCATCTGTGCC
TTCCGCCAAGGCCTGACCAAGGACGCACACAACGCCCTGCTGGACATCCAGTCGAGTGGC
CGAGCCAAGGAGCTTCTGGGCCAGGGCCTGCTGCTGCGCAGCCTGCAGGAGCGCAACCAG
GAGCAGGAGAAGGTGGAGCGGCGCCGTCAGGTCCCCTTCCACCTGCACATCAACCTGGAG
CTGCTGGAGTGTGTCTACCTGGTGTCTGCCATGCTCCTGGAGATCCCCTACATGGCCGCC
CATGAGAGCGATGCCCGCCGACGCATGATCAGCAAGCAGTTCCACCACCAGCTGCGCGTG
GGCGAGCGACAGCCCCTGCTGGGTCCCCCTGAGTCCATGCGGGAACATGTGGTCGCTGCC
TCCAAGGCCATGAAGATGGGTGACTGGAAGACCTGTCACAGTTTTATCATCAATGAGAAG
ATGAATGGGAAAGTGTGGGACCTTTTCCCCGAGGCTGACAAAGTCCGCACCATGCTGGTT
AGGAAGATCCAGGAAGAGTCACTGAGGACCTACCTCTTCACCTACAGCAGTGTCTATGAC
TCCATCAGCATGGAGACGCTGTCAGACATGTTTGAGCTGGATCTGCCCACTGTGCACTCC
ATCATCAGCAAAATGATCATTAATGAGGAGCTGATGGCCTCCCTGGACCAGCCAACACAG
ACAGTGGTGATGCACCGCACTGAGCCCACTGCCCAGCAGAACCTGGCTCTGCAGCTGGCC
GAGAAGCTGGGCAGCCTGGTGGAGAACAACGAACGGGTGTTTGACCACAAGCAGGGCACC
TACGGGGGCTACTTCCGAGACCAGAAGGACGGCTACCGCAAAAACGAGGGCTACATGCGC
CGCGGTGGCTACCGCCAGCAGCAGTCTCAGACGGCCTAC

Protein sequence predicted based on nucleotide sequence

MSRFFTTGSDSESESSLSGEELVTKPVGGNYGKQPLLLSEDEEDTKRVVRSAKDKRFEEL
TNLIRTIRNAMKIRDVTKCLEEFELLGKAYGKAKSIVDKEGVPRFYIRILADLEDYLNEL
WEDKEGKKKMNKNNAKALSTLRQKIRKYNRDFESHITSYKQNPEQSADEDAEKNEEDSEG
SSDEDEDEDGVSAATFLKKKSEAPSGESRKFLKKMDDEDEDSEDSEDDEDWDTGSTSSDS
DSEEEEGKQTALASRFLKKAPTTDEDKKAAEKKREDKAKKKHDRKSKRLDEEEEDNEGGE
WERVRGGVPLVKEKPKMFAKGTEITHAVVIKKLNEILQARGKKGTDRAAQIELLQLLVQI
AAENNLGEGVIVKIKFNIIASLYDYNPNLATYMKPEMWGKCLDCINELMDILFANPNIFV
GENILEESENLHNADQPLRVRGCILTLVERMDEEFTKIMQNTDPHSQEYVEHLKDEAQVC
AIIERVQRYLEEKGTTEEVCRIYLLRILHTYYKFDYKAHQRQLTPPEGSSKSEQDQAENE
GEDSAVLMERLCKYIYAKDRTDRIRTCAILCHIYHHALHSRWYQARDLMLMSHLQDNIQH
ADPPVQILYNRTMVQLGICAFRQGLTKDAHNALLDIQSSGRAKELLGQGLLLRSLQERNQ
EQEKVERRRQVPFHLHINLELLECVYLVSAMLLEIPYMAAHESDARRRMISKQFHHQLRV
GERQPLLGPPESMREHVVAASKAMKMGDWKTCHSFIINEKMNGKVWDLFPEADKVRTMLV
RKIQEESLRTYLFTYSSVYDSISMETLSDMFELDLPTVHSIISKMIINEELMASLDQPTQ
TVVMHRTEPTAQQNLALQLAEKLGSLVENNERVFDHKQGTYGGYFRDQKDGYRKNEGYMR
RGGYRQQQSQTAY

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable