Gene:
Gene Symbol:
RNF20
Gene Name:
ring finger protein 20, E3 ubiquitin protein ligase
Original Clone ID:
ccsbBroadEn_03713
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC110585.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505940
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTCAGGAATTGGAAATAAAAGAGCAGCTGGAGAACCTGGCACCTCCATGCCTCCTGAG
AAGAAGGCAGCTGTTGAAGATTCAGGGACCACAGTGGAAACAATTAAGCTAGGAGGTGTC
TCTTCAACGGAGGAACTAGACATTAGAACACTGCAAACCAAAAATCGCAAGCTGGCAGAA
ATGTTGGATCAGCGGCAGGCCATTGAAGATGAACTTCGTGAGCACATTGAAAAACTGGAA
CGACGACAGGCCACTGATGATGCCTCACTATTGATTGTCAACCGATACTGGAGTCAGTTT
GATGAAAACATCCGTATCATCCTTAAACGTTATGATCTGGAGCAGGGCTTGGGAGACCTA
CTCACAGAACGAAAAGCCCTTGTTGTGCCTGAACCAGAACCAGACTCTGATAGCAATCAG
GAGCGTAAAGATGACCGAGAGAGAGGGGAAGGGCAAGAGCCAGCTTTCTCTTTCCTTGCT
ACTTTGGCCAGCAGTTCCAGTGAAGAGATGGAGTCTCAGCTGCAGGAACGTGTGGAGTCT
TCCCGCCGAGCCGTGTCCCAGATTGTGACTGTTTATGATAAATTGCAAGAAAAAGTGGAG
CTCTTATCCCGGAAGCTAAACAGTGGAGATAATCTGATAGTGGAGGAAGCAGTGCAGGAG
CTGAACTCTTTCCTCGCACAGGAGAATATGAGGCTACAGGAATTGACAGATCTTCTTCAG
GAAAAGCATCGCACCATGTCTCAGGAGTTCTCCAAGTTGCAGAGTAAAGTGGAGACAGCC
GAATCACGAGTGTCTGTCCTGGAGTCCATGATTGATGACCTGCAGTGGGATATTGACAAA
ATTCGAAAGAGGGAACAGCGACTCAACCGACACTTAGCAGAAGTCCTAGAACGGGTGAAT
TCCAAAGGTTATAAGGTGTATGGAGCGGGGAGCAGTCTGTATGGCGGCACAATCACTATC
AATGCTCGGAAGTTTGAGGAAATGAATGCAGAGCTTGAGGAGAACAAAGAGTTGGCTCAG
AACCGTCTCTGTGAGCTGGAGAAACTTCGGCAAGACTTTGAGGAGGTCACTACACAAAAT
GAAAAGCTGAAGGTGGAATTGCGGAGTGCAGTGGAGCAAGTCGTTAAGGAAACTCCAGAA
TATCGCTGCATGCAGTCACAGTTCTCCGTCTTGTATAATGAGAGCCTACAGTTGAAAGCA
CACTTGGATGAGGCTCGGACCCTGCTTCATGGCACCAGAGGAACCCACCAGCACCAGGTT
GAGCTTATTGAGCGAGATGAGGTTAGTCTTCATAAGAAGCTGAGGACTGAAGTAATTCAG
CTAGAAGATACATTGGCCCAGGTCCGCAAGGAGTATGAAATGCTGAGGATAGAATTTGAG
CAGACCCTTGCTGCCAATGAACAAGCAGGCCCTATAAACAGGGAGATGCGCCACCTCATC
AGTAGCCTCCAGAATCACAATCACCAGCTGAAAGGGGAGGTCCTGAGATATAAGCGGAAA
TTGAGAGAAGCCCAGTCTGACCTGAACAAGACACGCCTGCGTAGTGGTAGTGCCCTCCTG
CAGTCCCAGTCTAGTACTGAGGACCCGAAGGATGAGCCTGCGGAGCTAAAACCAGATTCT
GAGGACTTATCCTCCCAGTCCTCAGCTTCAAAGGCATCTCAGGAGGATGCCAATGAAATC
AAGTCTAAACGGGATGAAGAAGAACGAGAACGAGAAAGGAGGGAGAAGGAGAGGGAACGA
GAAAGAGAACGGGAGAAGGAGAAGGAGAGAGAACGAGAGAAGCAGAAGCTAAAAGAGTCA
GAAAAAGAGAGAGATTCTGCTAAGGATAAAGAGAAAGGCAAACATGATGATGGACGGAAA
AAGGAAGCAGAAATTATCAAACAATTGAAGATTGAACTCAAGAAGGCACAGGAGAGCCAA
AAGGAGATGAAACTATTGCTGGATATGTACCGTTCTGCCCCAAAGGAACAGAGAGACAAA
GTTCAGCTGATGGCAGCTGAGAAGAAGTCTAAGGCAGAGTTGGAAGATCTAAGGCAAAGA
CTCAAGGATCTGGAAGATAAAGAGAAGAAAGAGAACAAGAAAATGGCTGATGAGGATGCC
TTGAGGAAGATCCGGGCAGTGGAGGAGCAGATAGAATACCTACAGAAGAAGCTAGCCATG
GCCAAGCAGGAAGAAGAAGCACTCCTCTCTGAAATGGATGTCACAGGCCAGGCCTTTGAA
GACATGCAGGAGCAAAATATCCGTTTGATGCAGCAATTGCGGGAGAAGGATGATGCAAAT
TTCAAGCTCATGTCAGAGCGTATCAAGTCCAATCAGATCCATAAGTTGCTTAAAGAAGAG
AAGGAGGAGCTGGCAGACCAGGTGTTGACTCTGAAGACTCAGGTTGATGCCCAGCTACAG
GTAGTAAGGAAACTGGAAGAGAAGGAGCATCTGTTACAGAGCAACATTGGCACAGGGGAG
AAAGAGCTGGGTCTTAGGACCCAAGCCTTAGAGATGAATAAACGCAAGGCAATGGAGGCA
GCCCAGCTTGCAGATGACCTCAAAGCACAACTGGAGTTGGCTCAGAAGAAGCTACATGAT
TTTCAGGATGAGATCGTGGAGAACAGTGTTACCAAAGAAAAGGACATGTTCAATTTCAAA
CGAGCCCAGGAGGACATCTCTAGACTTCGCAGGAAGCTGGAGACCACAAAGAAACCAGAC
AATGTACCCAAGTGTGATGAGATTCTGATGGAAGAGATTAAGGATTACAAGGCACGCTTG
ACCTGTCCGTGCTGTAACATGCGTAAAAAGGATGCTGTTCTTACTAAGTGTTTTCATGTC
TTCTGCTTTGAGTGTGTGAAGACACGCTATGACACCCGCCAGCGCAAATGTCCCAAGTGT
AATGCTGCTTTTGGTGCCAATGATTTTCATCGCATCTACATTGGT
Protein sequence predicted based on nucleotide sequence
MSGIGNKRAAGEPGTSMPPEKKAAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAE MLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDL LTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVES SRRAVSQIVTVYDKLQEKVELLSRKLNSGDNLIVEEAVQELNSFLAQENMRLQELTDLLQ EKHRTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVN SKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRLCELEKLRQDFEEVTTQN EKLKVELRSAVEQVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQHQV ELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLI SSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKPDS EDLSSQSSASKASQEDANEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKES EKERDSAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDK VQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAM AKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEE KEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEA AQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPD NVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKC NAAFGANDFHRIYIG
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
