DNASU Plasmid | RAD54B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: RAD54B

Gene Name: RAD54 homolog B (S. cerevisiae)

Original Clone ID:

ccsbBroadEn_02852

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC001965.2

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00505963

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2731nts

Open reading frame: 1 to 2731

Reference Sequence Alignment

ATGAGACGATCTGCAGCACCAAGTCAGTTGCAGGGGAATTCCTTCAAAAAACCAAAATTT
ATACCTCCAGGAAGAAGTAATCCAGGTCTGAATGAAGAGATTACAAAACTGAATCCAGAT
ATAAAATTATTTGAGGGTGTTGCAATTAATAACACCTTTCTCCCGTCACAAAATGATCTT
AGAATATGCAGTTTAAATCTGCCTAGTGAAGAAAGTACTAGAGAAATCAATAACAGAGAT
AATTGCAGTGGAAAATATTGTTTTGAAGCACCTACACTGGCAACATTAGATCCACCTCAT
ACAGTTCATTCGGCTCCTAAAGAAGTAGCAGTGTCCAAGGAACAAGAAGAGAAATCTGAT
AGCCTAGTTAAATATTTCAGTGTTGTTTGGTGTAAGCCTTCAAAGAAAAAACATAAAAAG
TGGGAAGGTGATGCTGTTCTTATTGTAAAAGGAAAGTCATTTATATTAAAGAATTTGGAA
GGCAAAGACATTGGAAGAGGCATTGGTTATAAATTCAAAGAGCTTGAAAAGATTGAAGAG
GGCCAAACACTGATGATTTGTGGAAAAGAAATAGAAGTCATGGGTGTAATCTCTCCAGAT
GACTTCAGCAGTGGCAGGTGTTTTCAGCTTGGAGGAGGAAGTACTGCTATCTCGCATTCT
TCTCAGGTTGCCAGGAAATGTTTCTCTAACCCTTTCAAAAGTGTTTGTAAACCAAGTTCA
AAGGAAAATAGACAGAATGATTTCCAAAATTGCAAACCACGCCATGACCCATATACGCCA
AATTCCCTCGTTATGCCACGACCAGATAAGAATCACCAGTGGGTATTCAATAAGAACTGT
TTCCCTCTTGTGGATGTAGTGATTGATCCTTACCTTGTATATCATCTTCGACCACATCAG
AAAGAAGGAATCATATTCCTTTATGAATGTGTAATGGGAATGAGAATGAATGGCAGATGT
GGAGCTATTCTTGCTGATGAAATGGGTTTAGGGAAGACATTGCAATGTATTTCGCTCATC
TGGACCCTGCAGTGTCAGGGACCCTATGGAGGCAAGCCAGTAATAAAGAAGACACTAATT
GTCACACCTGGAAGCTTGGTGAATAATTGGAAGAAAGAATTTCAAAAATGGCTAGGAAGT
GAAAGGATCAAGATATTTACTGTTGATCAGGACCACAAAGTTGAAGAATTCATCAAGTCT
ATATTTTATTCTGTTCTTATTATCAGTTATGAAATGTTACTTCGTTCCCTGGATCAAATT
AAGAATATAAAATTTGATCTTCTAATCTGTGACGAGGGGCATCGTTTGAAGAACAGTGCC
ATTAAGACAACTACAGCCCTCATTAGCCTTTCTTGTGAGAAAAGAATAATTCTAACTGGT
ACTCCAATTCAGAATGATCTGCAAGAATTTTTTGCATTAATTGATTTTGTAAATCCAGGA
ATATTAGGCTCTTTGTCATCTTATAGGAAAATATATGAAGAACCCATCATTTTATCGAGA
GAACCTTCTGCTTCTGAGGAAGAAAAGGAGTTAGGAGAAAGAAGAGCAGCTGAACTTACT
TGCCTCACTGGACTCTTTATCCTTAGAAGAACCCAAGAAATTATAAATAAATATCTCCCA
CCTAAAATAGAGAATGTTGTCTTTTGCCGACCAGGAGCACTACAGATTGAGCTTTATCGA
AAGCTGTTAAATTCTCAGGTTGTCAGGTTCTGCCTTCAAGGGTTGTTGGAAAATAGTCCC
CATCTAATATGTATAGGAGCTCTTAAAAAACTGTGCAATCACCCCTGCCTTTTGTTCAAC
TCTATAAAGGAAAAGGAATGTAGCTCAACTTGTGATAAAAATGAAGAAAAGAGTCTATAC
AAAGGCTTGCTAAGTGTGTTTCCTGCTGACTACAACCCTCTCCTGTTTACTGAAAAGGAG
TCAGGAAAACTACAGGTGTTGTCCAAGCTCTTAGCGGTTATCCACGAACTTCGACCTACT
GAAAAGGTGGTGTTGGTATCCAACTATACACAAACCTTGAACATTTTACAAGAAGTATGT
AAGCGTCATGGATATGCTTATACAAGACTTGATGGACAAACACCAATCTCTCAAAGGCAG
CAGATTGTTGATGGCTTTAACAGTCAACACTCTTCTTTTTTTATTTTTTTGTTAAGTTCA
AAAGCTGGTGGTGTAGGACTTAACCTCATTGGAGGATCTCACTTAATTCTCTATGACATT
GATTGGAATCCAGCCACTGACATTCAGGCAATGTCTAGAGTATGGAGAGATGGTCAGAAA
TATCCTGTACATATTTACAGACTCCTAACTACAGGTACAATAGAAGAAAAGATCTATCAA
AGGCAGATCAGTAAGCAAGGTCTTTGTGGGGCAGTTGTCGACCTCACCAAGACATCTGAA
CATATTCAGTTTTCAGTAGAAGAACTTAAAAATTTGTTCACATTACATGAAAGTTCAGAT
TGTGTTACTCATGATCTGCTTGACTGTGAGTGTACAGGAGAAGAAGTTCATACAGGTGAT
TCGTTGGAAAAATTCATTGTCTCTAGAGATTGTCAGCTTGGTCCACATCACCAGAAATCT
AACTCCCTGAAACCTCTTTCTATGTCCCAGCTGAAGCAATGGAAACATTTTTCTGGAGAT
CATTTAAATCTTACAGATCCTTTTCTTGAAAGAATAACAGAAAATGTGTCATTCATTTTT
CAGAATATAACCACTCAAGCTACTGGCACA

Protein sequence predicted based on nucleotide sequence

MRRSAAPSQLQGNSFKKPKFIPPGRSNPGLNEEITKLNPDIKLFEGVAINNTFLPSQNDL
RICSLNLPSEESTREINNRDNCSGKYCFEAPTLATLDPPHTVHSAPKEVAVSKEQEEKSD
SLVKYFSVVWCKPSKKKHKKWEGDAVLIVKGKSFILKNLEGKDIGRGIGYKFKELEKIEE
GQTLMICGKEIEVMGVISPDDFSSGRCFQLGGGSTAISHSSQVARKCFSNPFKSVCKPSS
KENRQNDFQNCKPRHDPYTPNSLVMPRPDKNHQWVFNKNCFPLVDVVIDPYLVYHLRPHQ
KEGIIFLYECVMGMRMNGRCGAILADEMGLGKTLQCISLIWTLQCQGPYGGKPVIKKTLI
VTPGSLVNNWKKEFQKWLGSERIKIFTVDQDHKVEEFIKSIFYSVLIISYEMLLRSLDQI
KNIKFDLLICDEGHRLKNSAIKTTTALISLSCEKRIILTGTPIQNDLQEFFALIDFVNPG
ILGSLSSYRKIYEEPIILSREPSASEEEKELGERRAAELTCLTGLFILRRTQEIINKYLP
PKIENVVFCRPGALQIELYRKLLNSQVVRFCLQGLLENSPHLICIGALKKLCNHPCLLFN
SIKEKECSSTCDKNEEKSLYKGLLSVFPADYNPLLFTEKESGKLQVLSKLLAVIHELRPT
EKVVLVSNYTQTLNILQEVCKRHGYAYTRLDGQTPISQRQQIVDGFNSQHSSFFIFLLSS
KAGGVGLNLIGGSHLILYDIDWNPATDIQAMSRVWRDGQKYPVHIYRLLTTGTIEEKIYQ
RQISKQGLCGAVVDLTKTSEHIQFSVEELKNLFTLHESSDCVTHDLLDCECTGEEVHTGD
SLEKFIVSRDCQLGPHHQKSNSLKPLSMSQLKQWKHFSGDHLNLTDPFLERITENVSFIF
QNITTQATGT

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

RAD54B

Symbol Nomenclature :

RAD54B

SYNONYM :

RDH54

Designation :

DNA repair and recombination protein RAD54B

Full Nomenclature :

RAD54 homolog B (S. cerevisiae)

GENEID :

25788

Locus Tag :

hCG_2009220

HGNC :

17228

MIM :

604289

Vega :

OTTHUMG00000133658

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00505963      ATGAGACGATCTGCAGCACCAAGTCAGTTGCAGGGGAATTCCTTCAAAAAACCAAAATTT
NM_012415.3       ATGAGACGATCTGCAGCACCAAGTCAGTTGCAGGGGAATTCCTTCAAAAAACCAAAATTT
                  ************************************************************

HsCD00505963      ATACCTCCAGGAAGAAGTAATCCAGGTCTGAATGAAGAGATTACAAAACTGAATCCAGAT
NM_012415.3       ATACCTCCAGGAAGAAGTAATCCAGGTCTGAATGAAGAGATTACAAAACTGAATCCAGAT
                  ************************************************************

HsCD00505963      ATAAAATTATTTGAGGGTGTTGCAATTAATAACACCTTTCTCCCGTCACAAAATGATCTT
NM_012415.3       ATAAAATTATTTGAGGGTGTTGCAATTAATAACACCTTTCTCCCGTCACAAAATGATCTT
                  ************************************************************

HsCD00505963      AGAATATGCAGTTTAAATCTGCCTAGTGAAGAAAGTACTAGAGAAATCAATAACAGAGAT
NM_012415.3       AGAATATGCAGTTTAAATCTGCCTAGTGAAGAAAGTACTAGAGAAATCAATAACAGAGAT
                  ************************************************************

HsCD00505963      AATTGCAGTGGAAAATATTGTTTTGAAGCACCTACACTGGCAACATTAGATCCACCTCAT
NM_012415.3       AATTGCAGTGGAAAATATTGTTTTGAAGCACCTACACTGGCAACATTAGATCCACCTCAT
                  ************************************************************

HsCD00505963      ACAGTTCATTCGGCTCCTAAAGAAGTAGCAGTGTCCAAGGAACAAGAAGAGAAATCTGAT
NM_012415.3       ACAGTTCATTCGGCTCCTAAAGAAGTAGCAGTGTCCAAGGAACAAGAAGAGAAATCTGAT
                  ************************************************************

HsCD00505963      AGCCTAGTTAAATATTTCAGTGTTGTTTGGTGTAAGCCTTCAAAGAAAAAACATAAAAAG
NM_012415.3       AGCCTAGTTAAATATTTCAGTGTTGTTTGGTGTAAGCCTTCAAAGAAAAAACATAAAAAG
                  ************************************************************

HsCD00505963      TGGGAAGGTGATGCTGTTCTTATTGTAAAAGGAAAGTCATTTATATTAAAGAATTTGGAA
NM_012415.3       TGGGAAGGTGATGCTGTTCTTATTGTAAAAGGAAAGTCATTTATATTAAAGAATTTGGAA
                  ************************************************************

HsCD00505963      GGCAAAGACATTGGAAGAGGCATTGGTTATAAATTCAAAGAGCTTGAAAAGATTGAAGAG
NM_012415.3       GGCAAAGACATTGGAAGAGGCATTGGTTATAAATTCAAAGAGCTTGAAAAGATTGAAGAG
                  ************************************************************

HsCD00505963      GGCCAAACACTGATGATTTGTGGAAAAGAAATAGAAGTCATGGGTGTAATCTCTCCAGAT
NM_012415.3       GGCCAAACACTGATGATTTGTGGAAAAGAAATAGAAGTCATGGGTGTAATCTCTCCAGAT
                  ************************************************************

HsCD00505963      GACTTCAGCAGTGGCAGGTGTTTTCAGCTTGGAGGAGGAAGTACTGCTATCTCGCATTCT
NM_012415.3       GACTTCAGCAGTGGCAGGTGTTTTCAGCTTGGAGGAGGAAGTACTGCTATCTCGCATTCT
                  ************************************************************

HsCD00505963      TCTCAGGTTGCCAGGAAATGTTTCTCTAACCCTTTCAAAAGTGTTTGTAAACCAAGTTCA
NM_012415.3       TCTCAGGTTGCCAGGAAATGTTTCTCTAACCCTTTCAAAAGTGTTTGTAAACCAAGTTCA
                  ************************************************************

HsCD00505963      AAGGAAAATAGACAGAATGATTTCCAAAATTGCAAACCACGCCATGACCCATATACGCCA
NM_012415.3       AAGGAAAATAGACAGAATGATTTCCAAAATTGCAAACCACGCCATGACCCATATACGCCA
                  ************************************************************

HsCD00505963      AATTCCCTCGTTATGCCACGACCAGATAAGAATCACCAGTGGGTATTCAATAAGAACTGT
NM_012415.3       AATTCCCTCGTTATGCCACGACCAGATAAGAATCACCAGTGGGTATTCAATAAGAACTGT
                  ************************************************************

HsCD00505963      TTCCCTCTTGTGGATGTAGTGATTGATCCTTACCTTGTATATCATCTTCGACCACATCAG
NM_012415.3       TTCCCTCTTGTGGATGTAGTGATTGATCCTTACCTTGTATATCATCTTCGACCACATCAG
                  ************************************************************

HsCD00505963      AAAGAAGGAATCATATTCCTTTATGAATGTGTAATGGGAATGAGAATGAATGGCAGATGT
NM_012415.3       AAAGAAGGAATCATATTCCTTTATGAATGTGTAATGGGAATGAGAATGAATGGCAGATGT
                  ************************************************************

HsCD00505963      GGAGCTATTCTTGCTGATGAAATGGGTTTAGGGAAGACATTGCAATGTATTTCGCTCATC
NM_012415.3       GGAGCTATTCTTGCTGATGAAATGGGTTTAGGGAAGACATTGCAATGTATTTCGCTCATC
                  ************************************************************

HsCD00505963      TGGACCCTGCAGTGTCAGGGACCCTATGGAGGCAAGCCAGTAATAAAGAAGACACTAATT
NM_012415.3       TGGACCCTGCAGTGTCAGGGACCCTATGGAGGCAAGCCAGTAATAAAGAAGACACTAATT
                  ************************************************************

HsCD00505963      GTCACACCTGGAAGCTTGGTGAATAATTGGAAGAAAGAATTTCAAAAATGGCTAGGAAGT
NM_012415.3       GTCACACCTGGAAGCTTGGTGAATAATTGGAAGAAAGAATTTCAAAAATGGCTAGGAAGT
                  ************************************************************

HsCD00505963      GAAAGGATCAAGATATTTACTGTTGATCAGGACCACAAAGTTGAAGAATTCATCAAGTCT
NM_012415.3       GAAAGGATCAAGATATTTACTGTTGATCAGGACCACAAAGTTGAAGAATTCATCAAGTCT
                  ************************************************************

HsCD00505963      ATATTTTATTCTGTTCTTATTATCAGTTATGAAATGTTACTTCGTTCCCTGGATCAAATT
NM_012415.3       ATATTTTATTCTGTTCTTATTATCAGTTATGAAATGTTACTTCGTTCCCTGGATCAAATT
                  ************************************************************

HsCD00505963      AAGAATATAAAATTTGATCTTCTAATCTGTGACGAGGGGCATCGTTTGAAGAACAGTGCC
NM_012415.3       AAGAATATAAAATTTGATCTTCTAATCTGTGACGAGGGGCATCGTTTGAAGAACAGTGCC
                  ************************************************************

HsCD00505963      ATTAAGACAACTACAGCCCTCATTAGCCTTTCTTGTGAGAAAAGAATAATTCTAACTGGT
NM_012415.3       ATTAAGACAACTACAGCCCTCATTAGCCTTTCTTGTGAGAAAAGAATAATTCTAACTGGT
                  ************************************************************

HsCD00505963      ACTCCAATTCAGAATGATCTGCAAGAATTTTTTGCATTAATTGATTTTGTAAATCCAGGA
NM_012415.3       ACTCCAATTCAGAATGATCTGCAAGAATTTTTTGCATTAATTGATTTTGTAAATCCAGGA
                  ************************************************************

HsCD00505963      ATATTAGGCTCTTTGTCATCTTATAGGAAAATATATGAAGAACCCATCATTTTATCGAGA
NM_012415.3       ATATTAGGCTCTTTGTCATCTTATAGGAAAATATATGAAGAACCCATCATTTTATCGAGA
                  ************************************************************

HsCD00505963      GAACCTTCTGCTTCTGAGGAAGAAAAGGAGTTAGGAGAAAGAAGAGCAGCTGAACTTACT
NM_012415.3       GAACCTTCTGCTTCTGAGGAAGAAAAGGAGTTAGGAGAAAGAAGAGCAGCTGAACTTACT
                  ************************************************************

HsCD00505963      TGCCTCACTGGACTCTTTATCCTTAGAAGAACCCAAGAAATTATAAATAAATATCTCCCA
NM_012415.3       TGCCTCACTGGACTCTTTATCCTTAGAAGAACCCAAGAAATTATAAATAAATATCTCCCA
                  ************************************************************

HsCD00505963      CCTAAAATAGAGAATGTTGTCTTTTGCCGACCAGGAGCACTACAGATTGAGCTTTATCGA
NM_012415.3       CCTAAAATAGAGAATGTTGTCTTTTGCCGACCAGGAGCACTACAGATTGAGCTTTATCGA
                  ************************************************************

HsCD00505963      AAGCTGTTAAATTCTCAGGTTGTCAGGTTCTGCCTTCAAGGGTTGTTGGAAAATAGTCCC
NM_012415.3       AAGCTGTTAAATTCTCAGGTTGTCAGGTTCTGCCTTCAAGGGTTGTTGGAAAATAGTCCC
                  ************************************************************

HsCD00505963      CATCTAATATGTATAGGAGCTCTTAAAAAACTGTGCAATCACCCCTGCCTTTTGTTCAAC
NM_012415.3       CATCTAATATGTATAGGAGCTCTTAAAAAACTGTGCAATCACCCCTGCCTTTTGTTCAAC
                  ************************************************************

HsCD00505963      TCTATAAAGGAAAAGGAATGTAGCTCAACTTGTGATAAAAATGAAGAAAAGAGTCTATAC
NM_012415.3       TCTATAAAGGAAAAGGAATGTAGCTCAACTTGTGATAAAAATGAAGAAAAGAGTCTATAC
                  ************************************************************

HsCD00505963      AAAGGCTTGCTAAGTGTGTTTCCTGCTGACTACAACCCTCTCCTGTTTACTGAAAAGGAG
NM_012415.3       AAAGGCTTGCTAAGTGTGTTTCCTGCTGACTACAACCCTCTCCTGTTTACTGAAAAGGAG
                  ************************************************************

HsCD00505963      TCAGGAAAACTACAGGTGTTGTCCAAGCTCTTAGCGGTTATCCACGAACTTCGACCTACT
NM_012415.3       TCAGGAAAACTACAGGTGTTGTCCAAGCTCTTAGCGGTTATCCACGAACTTCGACCTACT
                  ************************************************************

HsCD00505963      GAAAAGGTGGTGTTGGTATCCAACTATACACAAACCTTGAACATTTTACAAGAAGTATGT
NM_012415.3       GAAAAGGTGGTGTTGGTATCCAACTATACACAAACCTTGAACATTTTACAAGAAGTATGT
                  ************************************************************

HsCD00505963      AAGCGTCATGGATATGCTTATACAAGACTTGATGGACAAACACCAATCTCTCAAAGGCAG
NM_012415.3       AAGCGTCATGGATATGCTTATACAAGACTTGATGGACAAACACCAATCTCTCAAAGGCAG
                  ************************************************************

HsCD00505963      CAGATTGTTGATGGCTTTAACAGTCAACACTCTTCTTTTTTTATTTTTTTGTTAAGTTCA
NM_012415.3       CAGATTGTTGATGGCTTTAACAGTCAACACTCTTCTTTTTTTATTTTTTTGTTAAGTTCA
                  ************************************************************

HsCD00505963      AAAGCTGGTGGTGTAGGACTTAACCTCATTGGAGGATCTCACTTAATTCTCTATGACATT
NM_012415.3       AAAGCTGGTGGTGTAGGACTTAACCTCATTGGAGGATCTCACTTAATTCTCTATGACATT
                  ************************************************************

HsCD00505963      GATTGGAATCCAGCCACTGACATTCAGGCAATGTCTAGAGTATGGAGAGATGGTCAGAAA
NM_012415.3       GATTGGAATCCAGCCACTGACATTCAGGCAATGTCTAGAGTATGGAGAGATGGTCAGAAA
                  ************************************************************

HsCD00505963      TATCCTGTACATATTTACAGACTCCTAACTACAGGTACAATAGAAGAAAAGATCTATCAA
NM_012415.3       TATCCTGTACATATTTACAGACTCCTAACTACAGGTACAATAGAAGAAAAGATCTATCAA
                  ************************************************************

HsCD00505963      AGGCAGATCAGTAAGCAAGGTCTTTGTGGGGCAGTTGTCGACCTCACCAAGACATCTGAA
NM_012415.3       AGGCAGATCAGTAAGCAAGGTCTTTGTGGGGCAGTTGTCGACCTCACCAAGACATCTGAA
                  ************************************************************

HsCD00505963      CATATTCAGTTTTCAGTAGAAGAACTTAAAAATTTGTTCACATTACATGAAAGTTCAGAT
NM_012415.3       CATATTCAGTTTTCAGTAGAAGAACTTAAAAATTTGTTCACATTACATGAAAGTTCAGAT
                  ************************************************************

HsCD00505963      TGTGTTACTCATGATCTGCTTGACTGTGAGTGTACAGGAGAAGAAGTTCATACAGGTGAT
NM_012415.3       TGTGTTACTCATGATCTGCTTGACTGTGAGTGTACAGGAGAAGAAGTTCATACAGGTGAT
                  ************************************************************

HsCD00505963      TCGTTGGAAAAATTCATTGTCTCTAGAGATTGTCAGCTTGGTCCACATCACCAGAAATCT
NM_012415.3       TCGTTGGAAAAATTCATTGTCTCTAGAGATTGTCAGCTTGGTCCACATCACCAGAAATCT
                  ************************************************************

HsCD00505963      AACTCCCTGAAACCTCTTTCTATGTCCCAGCTGAAGCAATGGAAACATTTTTCTGGAGAT
NM_012415.3       AACTCCCTGAAACCTCTTTCTATGTCCCAGCTGAAGCAATGGAAACATTTTTCTGGAGAT
                  ************************************************************

HsCD00505963      CATTTAAATCTTACAGATCCTTTTCTTGAAAGAATAACAGAAAATGTGTCATTCATTTTT
NM_012415.3       CATTTAAATCTTACAGATCCTTTTCTTGAAAGAATAACAGAAAATGTGTCATTCATTTTT
                  ************************************************************

HsCD00505963      CAGAATATAACCACTCAAGCTACTGGCACA---
NM_012415.3       CAGAATATAACCACTCAAGCTACTGGCACATAG
                  ******************************