Gene:
Gene Symbol:
SETD5
Gene Name:
SET domain containing 5
Original Clone ID:
ccsbBroadEn_12183
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC020956.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505965
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCATGCTTTTGAAAACTTAGAGAAAAGAAAGAAGCGGCGGGATCAGCCCTTGGAACAG
AGCAACTCTGATGTAGAGATTACTACAACCACCTCAGAGACTCCTGTTGGTGAAGAGACA
AAAACTGAAGCCCCTGAATCTGAAGTTAGCAACTCTGTTTCAAATGTTACCATCCCAAGC
ACCCCACAGAGTGTTGGTGTGAATACCCGGAGGTCTTCCCAAGCAGGGGATATTGCTGCA
GAAAAACTAGTCCCCAAGCCACCTCCAGCAAAGCCTTCTAGGCCCCGGCCGAAGAGTCGA
ATTTCTCGGTACAGGACCAGTTCAGCCCAAAGACTAAAGCGTCAGAAGCAGGCCAATGCA
CAGCAGGCAGAATTGTCACAAGCTGCCTTGGAAGAGGGAGGAAGTAACAGTTTAGTAACT
CCTACTGAAGCTGGAAGTCTAGACAGTTCAGGAGAAAACAGGCCATTAACAGGGTCTGAC
CCAACTGTGGTGTCAATTACTGGATCCCATGTCAACCGTGCTGCATCTAAATACCCCAAA
ACCAAAAAGTATCTAGTTACAGAATGGTTGAATGACAAAGCAGAGAAGCAAGAGTGCCCT
GTTGAGTGCCCTTTACGTATCACAACGGATCCAACTGTACTGGCAACGACCCTAAACATG
TTACCAGGTCTTATCCATTCCCCGTTAATTTGCACCACCCCCAAACACTACATTCGCTTT
GGCTCACCCTTTATCCCTGAGAGACGTCGAAGGCCCCTTCTGCCTGATGGCACATTCAGC
TCCTGTAAGAAGCGCTGGATAAAACAAGCCTTAGAAGAAGGGATGACTCAAACATCATCT
GTACCCCAAGAGACTAGAACTCAGCACCTATACCAAAGCAATGAGAATAGTAGCTCTTCT
AGTATCTGCAAAGACAATGCAGACTTGTTGAGCCCATTAAAGAAATGGAAGTCTCGCTAT
CTGATGGAGCAGAATGTCACCAAGTTACTTCGGCCTCTGTCTCCAGTCACACCACCCCCT
CCCAATTCAGGCTCAAAGAGTCCCCAGCTGGCCACACCTGGCTCATCTCACCCAGGAGAA
GAGGAGTGTCGAAATGGATACAGCCTCATGTTTTCACCAGTCACATCTCTTACTACTGCT
AGTCGCTGCAACACTCCTCTACAGTTTGAGCTTTGTCACCGAAAAGACCTGGATTTGGCA
AAAGTAGGATACCTTGACTCCAACACTAACAGCTGTGCTGATAGACCTTCCCTACTCAAC
TCAGGTCATTCTGACCTGGCTCCTCATCCCTCCCTCGGACCCACTTCTGAGACTGGTTTC
CCAAGCAGAAGTGGAGATGGACATCAGACCCTCGTGAGAAACTCAGACCAGGCATTTCGG
ACAGAGTTCAACTTGATGTATGCCTACTCCCCTTTGAATGCTATGCCTCGAGCAGATGGA
CTGTATCGAGGATCTCCTCTAGTGGGGGATAGGAAGCCTTTACATTTGGATGGGGGATAT
TGTTCCCCTGCAGAAGGATTTTCCAGCAGATATGAACATGGCTTAATGAAAGACCTCTCT
CGTGGATCCTTGTCACCTGGTGGTGAAAGGGCCTGTGAAGGAGTCCCATCTGCCCCCCAG
AACCCACCACAGAGGAAAAAAGTATCCCTGCTGGAGTACCGAAAACGGAAACAAGAAGCT
AAGGAAAATTCTGCTGGTGGGGGAGGTGACTCTGCACAGAGCAAAAGCAAGTCTGCAGGA
GCTGGGCAAGGCAGCAGTAACTCCGTTTCCGACACTGGTGCCCATGGTGTGCAGGGATCC
TCAGCCCGAACTCCATCTTCCCCTCACAAAAAATTCTCCCCATCTCATTCCTCTATGTCC
CATTTGGAGGCGGTAAGCCCATCAGATTCCAGAGGCACTTCTTCATCTCACTGCAGACCT
CAAGAGAATATCAGCAGTAGGTGGATGGTTCCCACATCAGTAGAACGACTCCGAGAAGGA
GGGAGCATCCCCAAGGTCCTCCGAAGCAGCGTGAGGGTGGCCCAAAAGGGAGAGCCCTCT
CCCACATGGGAGAGTAACATCACAGAGAAAGACTCAGACCCTGCAGATGGAGAAGGCCCA
GAGACATTAAGCTCAGCACTCTCTAAAGGAGCAACAGTTTACAGCCCTTCCAGATACAGC
TACCAGCTCCTGCAGTGTGATAGTCCTCGGACAGAATCACAAAGCCTCCTTCAGCAGAGT
TCCTCCCCCTTCAGAGGACATCCTACACAGTCTCCAGGATACAGTTATCGAACTACTGCA
CTGAGACCTGGAAACCCCCCCTCTCACGGTTCTTCAGAATCATCCCTCTCTTCCACGTCC
TATTCCAGCCCCGCCCACCCTGTGTCCACAGACTCGTTGGCCCCATTTACGGGGACACCA
GGGTATTTTAGCAGCCAGCCACATTCTGGAAACAGCACTGGCAGCAATCTTCCAAGGAGG
AGCTGCCCTTCTAGTGCTGCTAGCCCTACCCTGCAGGGACCCTCAGACTCGCCAACCTCA
GATTCAGTTTCTCAGTCCAGCACAGGAACTCTGAGTTCCACCTCCTTTCCTCAGAACTCT
AGGTCGTCATTGCCATCAGACTTACGGACTATCAGTCTGCCCAGTGCTGGGCAGTCAGCT
GTCTACCAGGCCTCCAGGGTATCTGCGGTTTCCAATTCACAGCACTACCCACACCGTGGG
AGTGGGGGTGTGCACCAGTACCGACTCCAGCCACTGCAAGGGTCAGGAGTCAAGACTCAG
ACGGGACTTTCC
Protein sequence predicted based on nucleotide sequence
MHAFENLEKRKKRRDQPLEQSNSDVEITTTTSETPVGEETKTEAPESEVSNSVSNVTIPS TPQSVGVNTRRSSQAGDIAAEKLVPKPPPAKPSRPRPKSRISRYRTSSAQRLKRQKQANA QQAELSQAALEEGGSNSLVTPTEAGSLDSSGENRPLTGSDPTVVSITGSHVNRAASKYPK TKKYLVTEWLNDKAEKQECPVECPLRITTDPTVLATTLNMLPGLIHSPLICTTPKHYIRF GSPFIPERRRRPLLPDGTFSSCKKRWIKQALEEGMTQTSSVPQETRTQHLYQSNENSSSS SICKDNADLLSPLKKWKSRYLMEQNVTKLLRPLSPVTPPPPNSGSKSPQLATPGSSHPGE EECRNGYSLMFSPVTSLTTASRCNTPLQFELCHRKDLDLAKVGYLDSNTNSCADRPSLLN SGHSDLAPHPSLGPTSETGFPSRSGDGHQTLVRNSDQAFRTEFNLMYAYSPLNAMPRADG LYRGSPLVGDRKPLHLDGGYCSPAEGFSSRYEHGLMKDLSRGSLSPGGERACEGVPSAPQ NPPQRKKVSLLEYRKRKQEAKENSAGGGGDSAQSKSKSAGAGQGSSNSVSDTGAHGVQGS SARTPSSPHKKFSPSHSSMSHLEAVSPSDSRGTSSSHCRPQENISSRWMVPTSVERLREG GSIPKVLRSSVRVAQKGEPSPTWESNITEKDSDPADGEGPETLSSALSKGATVYSPSRYS YQLLQCDSPRTESQSLLQQSSSPFRGHPTQSPGYSYRTTALRPGNPPSHGSSESSLSSTS YSSPAHPVSTDSLAPFTGTPGYFSSQPHSGNSTGSNLPRRSCPSSAASPTLQGPSDSPTS DSVSQSSTGTLSSTSFPQNSRSSLPSDLRTISLPSAGQSAVYQASRVSAVSNSQHYPHRG SGGVHQYRLQPLQGSGVKTQTGLS
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
