Gene:
Gene Symbol:
PCDH10
Gene Name:
protocadherin 10
Original Clone ID:
ccsbBroadEn_08745
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC111560.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505992
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGATTGTGCTATTATTGTTTGCCTTGCTCTGGATGGTGGAAGGAGTCTTTTCCCAGCTT
CACTACACGGTACAGGAGGAGCAGGAACATGGCACTTTCGTGGGGAATATCGCTGAAGAT
CTGGGTCTGGACATTACAAAACTTTCGGCTCGCGGGTTTCAGACGGTGCCCAACTCAAGG
ACCCCTTACTTAGACCTCAACCTGGAGACAGGGGTGCTGTACGTGAACGAGAAAATAGAC
CGCGAACAAATCTGCAAACAGAGCCCCTCCTGTGTCCTGCACCTGGAGGTCTTTCTGGAG
AACCCCCTGGAGCTGTTCCAGGTGGAGATCGAGGTGCTGGACATTAATGACAACCCCCCC
TCTTTCCCGGAGCCAGACCTGACGGTGGAAATCTCTGAGAGCGCCACGCCAGGCACTCGC
TTCCCCTTGGAGAGCGCATTCGACCCAGACGTGGGCACCAACTCCTTGCGCGACTACGAG
ATCACCCCCAACAGCTACTTCTCCCTGGACGTGCAGACCCAGGGGGATGGCAACCGATTC
GCTGAGCTGGTGCTGGAGAAGCCACTGGACCGAGAGCAGCAAGCGGTGCACCGCTACGTG
CTGACCGCGGTGGACGGAGGAGGTGGGGGAGGAGTAGGAGAAGGAGGGGGAGGTGGCGGG
GGAGCAGGCCTGCCCCCCCAGCAGCAGCGCACCGGCACGGCCCTACTCACCATCCGAGTG
CTGGACTCCAATGACAATGTGCCCGCTTTCGACCAACCCGTCTACACTGTGTCCCTACCA
GAGAACTCTCCCCCAGGCACTCTCGTGATCCAGCTCAACGCCACCGACCCGGACGAGGGC
CAGAACGGTGAGGTCGTGTACTCCTTCAGCAGCCACATTTCGCCCCGGGCGCGGGAGCTT
TTCGGACTCTCGCCGCGCACTGGCAGACTGGAGGTAAGCGGCGAGTTGGACTATGAAGAG
AGCCCAGTGTACCAAGTGTACGTGCAAGCCAAGGACCTGGGCCCCAACGCCGTGCCTGCG
CACTGCAAGGTGCTAGTGCGAGTACTGGATGCTAATGACAACGCGCCAGAGATCAGCTTC
AGCACCGTGAAGGAAGCGGTGAGTGAGGGCGCGGCGCCCGGCACTGTGGTGGCCCTTTTC
AGCGTGACTGACCGCGACTCAGAGGAGAATGGGCAGGTGCAGTGCGAGCTACTGGGAGAC
GTGCCTTTCCGCCCCAAGTCTTCCTTTAAGAATTACTACACCATCGTTACCGAAGCCCCC
CTGGACCGAGAGGCGGGGGACTCCTACACCCTGACTGTAGTGGCTCGGGACCGGGGCGAG
CCTGCGCTCTCCACCAGTAAGTCGATCCAGGTACAAGTGTCGGATGTGAACGACAACGCG
CCGCGTTTCAGCCAGCCGGTCTACGACGTGTATGTGACTGAAAACAACGTGCCTGGCGCC
TACATCTACGCGGTGAGCGCCACCGACCGGGATGAGGGCGCCAACGCCCAGCTTGCCTAC
TCTATCCTCGAGTGCCAGATCCAGGGCATGAGCGTCTTCACCTACGTTTCTATCAACTCT
GAGAACGGCTACTTGTACGCCCTGCGCTCCTTCGACTATGAGCAGCTGAAGGACTTCAGT
TTTCAGGTGGAAGCCCGGGACGCTGGCAGCCCCCAGGCGCTGGCTGGTAACGCCACTGTC
AACATCCTCATAGTGGATCAAAATGACAACGCCCCTGCCATCGTGGCGCCTCTACCAGGG
CGCAACGGGACTCCAGCGCGTGAGGTGCTGCCCCGCTCGGCGGAGCCGGGTTACCTGCTC
ACCCGCGTGGCCGCCGTGGACGCGGACGACGGCGAGAACGCCCGGCTCACTTACAGCATC
GTGCGTGGCAACGAAATGAACCTCTTTCGCATGGACTGGCGCACCGGGGAGCTGCGCACA
GCACGCCGAGTCCCGGCCAAGCGCGACCCCCAGCGGCCTTATGAGCTGGTGATCGAGGTG
CGCGACCATGGGCAGCCGCCCCTTTCCTCCACCGCCACCCTGGTGGTTCAGCTGGTGGAT
GGCGCCGTGGAGCCCCAGGGCGGGGGCGGGAGCGGAGGCGGAGGGTCAGGAGAGCACCAG
CGCCCCAGTCGCTCTGGCGGCGGGGAAACCTCGCTAGACCTCACCCTCATCCTCATCATC
GCGTTGGGCTCGGTGTCCTTCATCTTCCTGCTGGCCATGATCGTGCTGGCCGTGCGTTGC
CAAAAAGAGAAGAAGCTCAACATCTATACTTGTCTGGCCAGCGATTGCTGCCTCTGCTGC
TGCTGCTGCGGTGGCGGAGGTTCGACCTGCTGTGGCCGCCAAGCCCGGGCGCGCAAGAAG
AAACTCAGCAAGTCAGACATCATGCTGGTGCAGAGCTCCAATGTACCCAGTAACCCGGCC
CAGGTGCCGATAGAGGAGTCCGGGGGCTTTGGCTCCCACCACCACAACCAGAATTACTGC
TATCAGGTATGCCTGACCCCTGAGTCCGCCAAGACCGACCTGATGTTTCTTAAGCCCTGC
AGCCCTTCGCGGAGTACGGACACTGAGCACAACCCCTGCGGGGCCATCGTCACCGGTTAC
ACCGACCAGCAGCCTGATATCATCTCCAACGGAAGCATTTTGTCCAACGAGACTAAACAC
CAGCGAGCAGAGCTCAGCTATCTAGTTGACAGACCTCGCCGAGTTAACAGTTCTGCATTC
CAGGAAGCCGACATAGTAAGCTCTAAGGACAGTGGTCATGGAGACAGTGAACAGGGAGAT
AGTGATCATGATGCCACCAACCGTGCCCAGTCAGCTGGTATGGATCTCTTCTCCAATTGC
ACTGAGGAATGTAAAGCTCTGGGCCACTCAGATCGGTGCTGGATGCCTTCTTTTGTCCCT
TCTGATGGACGCCAGGCTGCTGATTATCGCAGCAATCTGCATGTTCCTGGCATGGACTCT
GTTCCAGACACTGAGGTGTTTGAAACTCCAGAAGCCCAGCCTGGGGCAGAGCGGTCCTTT
TCCACCTTTGGCAAAGAGAAGGCCCTTCACAGCACTCTGGAGAGGAAGGAGCTGGATGGA
CTGCTGACTAATACGCGAGCGCCTTACAAACCACCATATTTGACACGGAAAAGGATATGC
Protein sequence predicted based on nucleotide sequence
MIVLLLFALLWMVEGVFSQLHYTVQEEQEHGTFVGNIAEDLGLDITKLSARGFQTVPNSR TPYLDLNLETGVLYVNEKIDREQICKQSPSCVLHLEVFLENPLELFQVEIEVLDINDNPP SFPEPDLTVEISESATPGTRFPLESAFDPDVGTNSLRDYEITPNSYFSLDVQTQGDGNRF AELVLEKPLDREQQAVHRYVLTAVDGGGGGGVGEGGGGGGGAGLPPQQQRTGTALLTIRV LDSNDNVPAFDQPVYTVSLPENSPPGTLVIQLNATDPDEGQNGEVVYSFSSHISPRAREL FGLSPRTGRLEVSGELDYEESPVYQVYVQAKDLGPNAVPAHCKVLVRVLDANDNAPEISF STVKEAVSEGAAPGTVVALFSVTDRDSEENGQVQCELLGDVPFRPKSSFKNYYTIVTEAP LDREAGDSYTLTVVARDRGEPALSTSKSIQVQVSDVNDNAPRFSQPVYDVYVTENNVPGA YIYAVSATDRDEGANAQLAYSILECQIQGMSVFTYVSINSENGYLYALRSFDYEQLKDFS FQVEARDAGSPQALAGNATVNILIVDQNDNAPAIVAPLPGRNGTPAREVLPRSAEPGYLL TRVAAVDADDGENARLTYSIVRGNEMNLFRMDWRTGELRTARRVPAKRDPQRPYELVIEV RDHGQPPLSSTATLVVQLVDGAVEPQGGGGSGGGGSGEHQRPSRSGGGETSLDLTLILII ALGSVSFIFLLAMIVLAVRCQKEKKLNIYTCLASDCCLCCCCCGGGGSTCCGRQARARKK KLSKSDIMLVQSSNVPSNPAQVPIEESGGFGSHHHNQNYCYQVCLTPESAKTDLMFLKPC SPSRSTDTEHNPCGAIVTGYTDQQPDIISNGSILSNETKHQRAELSYLVDRPRRVNSSAF QEADIVSSKDSGHGDSEQGDSDHDATNRAQSAGMDLFSNCTEECKALGHSDRCWMPSFVP SDGRQAADYRSNLHVPGMDSVPDTEVFETPEAQPGAERSFSTFGKEKALHSTLERKELDG LLTNTRAPYKPPYLTRKRIC
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
