Gene:
Gene Symbol:
ZBED4
Gene Name:
zinc finger, BED-type containing 4
Original Clone ID:
ccsbBroadEn_07504
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC117670.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00505999
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGAATAACTTGAAAACTTGTCCCAAAGAGGACGGTGATTTCGTTTCTGATAAAATA
AAGTTTAAAATAGAAGAGGAAGATGATGATGGAATTCCTCCTGATAGTTTGGAAAGAATG
GACTTTAAAAGCGAGCAGGAGGACATGAAGCAGACAGACAGCGGTGGGGAGCGAGCGGGC
CTCGGTGGGACGGGTTGCAGCTGCAAGCCCCCTGGGAAGTACTTGTCTGCAGAGAGTGAG
GATGACTATGGCGCGCTGTTCTCCCAGTACAGCAGCACCCTATACGACGTGGCCATGGAG
GCCGTGACCCAGAGCCTCCTTTCCAGCCGGAACATGAGCTCCAGGAAGAAGTCTCCAGCC
TGGAAGCATTTTTTTATCTCTCCCCGAGACAGCACTAAAGCAATATGCATGTACTGTGTG
AAGGAGTTCAGCAGAGGCAAAAACGAGAAAGACTTGAGTACCAGTTGTCTCATGAGGCAC
GTGAGGCGCGCACACCCGACCGTGCTCATTCAGGAAAATGGCAGCGTGTCTGCCGTGTCC
TCGTTCCCCTCTCCCTCACTCCTGCTTCCACCGCAGCCTGCGGACGCGGGTGACCTCAGC
ACCATCCTCTCACCCATCAAACTTGTCCAGAAAGTGGCGTCTAAGATCCCGTCCCCCGAT
CGAATAACAGAGGAGTCTGTGTCTGTAGTTTCTTCTGAAGAAATCTCCTCTGACATGTCC
GTTTCGGAGAAGTGCGGCAGAGAAGAAGCCCTGGTGGGGTCGTCTCCCCACCTCCCTGCT
CTCCATTACGATGAACCTGCAGAGAACTTAGCGGAGAAGAGCCTTCCACTTCCAAAGAGC
ACCTCTGGGTCCAGGAGAAGGTCCGCTGTCTGGAAGCACTTCTACCTGTCGCCACTGGAC
AACTCCAAAGCTGTCTGCATTCACTGCATGAACGAGTTCAGCCGGGGGAAGAATGGGAAG
GACCTGGGCACGAGCTGCCTCATCAGGCACATGTGGAGGGCACACCGCGCCATCGTGTTG
CAGGAGAACGGGGGCACGGGCATCCCGCCACTGTACTCCACCCCTCCCACTCTGCTGCCT
TCCTTGCTGCCGCCGGAGGGGGAGCTCAGCTCTGTGTCCTCGTCTCCGGTAAAGCCGGTC
AGAGAGTCCCCTTCGGCCTCCTCCTCCCCTGACAGGCTGACTGAGGACTTGCAGTCTCAC
TTGAACCCTGGAGATGGGCTGATGGAAGACGTGGCGGCCTTCTCATCTTCCGATGACGTA
GGGGAGGCCTCGGCGTCCTCTCCTGAGAAGCAGCAGGCTGATGGGCTGAGTCCTAGATTG
TTTGAATCTGGTGCCATCTTCCAGCAGAATAAAAAGGTCATGAAAAGACTGAAGTCGGAG
GTCTGGCATCACTTCTCCCTGGCCCCCATGGACAGCCTCAAGGCCGAGTGTCGGTACTGC
GGCTGTGCCATCAGCCGGGGGAAGAAGGGTGATGTGGGCACCAGCTGCCTGATGAGACAT
CTCTACCGGCGCCATCCAGAAGTTGTCGGGAGCCAGAAGGGCTTCCTGGGTGCAAGTCTG
GCAAACTCTCCGTATGCCACTTTGGCCTCTGCAGAAAGTTCCTCTTCCAAATTGACTGAC
TTGCCAACAGTGGTCACAAAAAACAATCAAGTTATGTTTCCTGTTAATAGCAAAAAGACC
TCGAAGCTGTGGAATCATTTTTCTATTTGCTCCGCAGACTCCACAAAAGTCGTGTGCTTG
CACTGTGGCCAGACCATCAGCCGGGGGAAGAAGCCGACTAACTTGGGTACCAGCTGTCTT
CTGAGACATTTACAGCGGTTCCATAGCAACGTGCTGAAAACTGAGGTCTCGGAGACGGCT
CGGCCCTCCTCTCCGGACACCCGGGTGCCGCGGGGCACAGAATTATCAGGCGCTTCCTCT
TTTGATGACACCAATGAGAAGTTTTACGATTCTCACCCAGTTGCCAAAAAAATCACAAGT
CTCATAGCTGAAATGATTGCACTTGACCTCCAGCCATATTCTTTTGTAGACAACGTTGGC
TTTAACAGGCTGCTTGAATACTTGAAACCTCAGTACTCCCTCCCCGCCCCTTCCTACTTC
TCCAGGACAGCTATCCCAGGTATGTATGATAATGTGAAGCAGATAATTATGTCCCACCTT
AAGGAAGCTGAGAGTGGTGTGATCCACTTCACGTCTGGAATATGGATGAGTAACCAGACC
CGTGAGTACCTGACCCTCACGGCCCACTGGGTTTCCTTCGAGTCGCCAGCCCGGCCGCGC
TGTGACGACCACCACTGCTCGGCGCTGTTGGACGTGTCGCAGGTGGACTGCGACTACAGT
GGCAACAGCATTCAGAAGCAGCTGGAGTGCTGGTGGGAAGCGTGGGTGACCTCCACCGGC
CTTCAGGTGGGCATCACCGTCACCGACAACGCCAGCATCGGGAAGACGCTGAACGAGGGG
GAGCACTCGAGCGTGCAGTGCTTCAGCCATACGGTGAACCTGATCGTCAGCGAGGCCATT
AAGAGCCAGCGGATGGTGCAGAACCTGCTGAGCCTCGCCCGGAAGATCTGCGAGCGGGTG
CACCGGTCGCCCAAGGCGAAGGAGAAACTGGCCGAGCTGCAGAGGGAGTACGCGCTGCCT
CAGCATCACCTCATCCAGGACGTCCCGTCCAAGTGGAGCACTTCCTTCCACATGCTCGAG
CGGCTCATTGAGCAGAAAAGGGCCATTAACGAGATGTCCGTCGAGTGTAACTTCCGAGAG
CTGATCAGCTGCGACCAGTGGGAGGTCATGCAGTCCGTGTGCCGTGCGCTAAAGCCCTTC
GAGGCCGCGAGCCGGGAGATGAGCACGCAGATGTCCACCCTCAGCCAGGTCATCCCCATG
GTACACATCCTCAACAGGAAGGTGGAGATGCTCTTCGAGGAGACGATGGGCATCGACACC
ATGCTGCGCTCTCTGAAGGAGGCCATGGTGAGCCGCCTGTCTGCCACCCTCCACGACCCG
CGGTACGTCTTCGCCACGCTGCTGGATCCTCGCTACAAGGCCTCCCTGTTTACGGAGGAG
GAGGCGGAGCAGTACAAACAGGATTTAATCAGGGAACTCGAACTCATGAATTCTACCTCA
GAGGACGTGGCTGCCTCCCACAGGTGTGATGCTGGCTCCCCGTCGAAAGACTCTGCCGCA
GAGGAGAACCTGTGGTCACTTGTGGCCAAGGTGAAGAAGAAAGACCCAAGAGAAAAGCTG
CCTGAAGCCATGGTGCTTGCGTATCTGGAGGAGGAGGTGCTTGAACACAGCTGTGACCCG
CTCACCTACTGGAACCTGAAGAAGGCGTCCTGGCCGGGGCTGTCCGCGCTGGCCGTCAGA
TTTTTGGGCTGCCCCCCAAGCATCGTCCCTTCAGAAAAGCTGTTCAACACACCCACTGAG
AACGGTAGCCTTGGCCAATCCAGGCTCATGATGGAACATTTTGAAAAACTTATCTTTTTG
AAAGTGAATCTTCCCTTAATATACTTTCAGTAT
Protein sequence predicted based on nucleotide sequence
MENNLKTCPKEDGDFVSDKIKFKIEEEDDDGIPPDSLERMDFKSEQEDMKQTDSGGERAG LGGTGCSCKPPGKYLSAESEDDYGALFSQYSSTLYDVAMEAVTQSLLSSRNMSSRKKSPA WKHFFISPRDSTKAICMYCVKEFSRGKNEKDLSTSCLMRHVRRAHPTVLIQENGSVSAVS SFPSPSLLLPPQPADAGDLSTILSPIKLVQKVASKIPSPDRITEESVSVVSSEEISSDMS VSEKCGREEALVGSSPHLPALHYDEPAENLAEKSLPLPKSTSGSRRRSAVWKHFYLSPLD NSKAVCIHCMNEFSRGKNGKDLGTSCLIRHMWRAHRAIVLQENGGTGIPPLYSTPPTLLP SLLPPEGELSSVSSSPVKPVRESPSASSSPDRLTEDLQSHLNPGDGLMEDVAAFSSSDDV GEASASSPEKQQADGLSPRLFESGAIFQQNKKVMKRLKSEVWHHFSLAPMDSLKAECRYC GCAISRGKKGDVGTSCLMRHLYRRHPEVVGSQKGFLGASLANSPYATLASAESSSSKLTD LPTVVTKNNQVMFPVNSKKTSKLWNHFSICSADSTKVVCLHCGQTISRGKKPTNLGTSCL LRHLQRFHSNVLKTEVSETARPSSPDTRVPRGTELSGASSFDDTNEKFYDSHPVAKKITS LIAEMIALDLQPYSFVDNVGFNRLLEYLKPQYSLPAPSYFSRTAIPGMYDNVKQIIMSHL KEAESGVIHFTSGIWMSNQTREYLTLTAHWVSFESPARPRCDDHHCSALLDVSQVDCDYS GNSIQKQLECWWEAWVTSTGLQVGITVTDNASIGKTLNEGEHSSVQCFSHTVNLIVSEAI KSQRMVQNLLSLARKICERVHRSPKAKEKLAELQREYALPQHHLIQDVPSKWSTSFHMLE RLIEQKRAINEMSVECNFRELISCDQWEVMQSVCRALKPFEAASREMSTQMSTLSQVIPM VHILNRKVEMLFEETMGIDTMLRSLKEAMVSRLSATLHDPRYVFATLLDPRYKASLFTEE EAEQYKQDLIRELELMNSTSEDVAASHRCDAGSPSKDSAAEENLWSLVAKVKKKDPREKL PEAMVLAYLEEEVLEHSCDPLTYWNLKKASWPGLSALAVRFLGCPPSIVPSEKLFNTPTE NGSLGQSRLMMEHFEKLIFLKVNLPLIYFQY
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
