Gene:
Gene Symbol:
ARHGEF10L
Gene Name:
Rho guanine nucleotide exchange factor (GEF) 10-like
Original Clone ID:
ccsbBroadEn_08479
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC117171.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00506001
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCTTCCTCCAACCCTCCTCCACAGCCTGCCATAGGAGATCAGCTGGTTCCAGGAGTC
CCAGGCCCCTCCTCTGAGGCAGAGGACGACCCAGGAGAGGCGTTTGAGTTTGATGACAGT
GATGATGAAGAGGACACCAGCGCAGCCCTGGGCGTCCCCAGCCTTGCTCCTGAGAGGGAC
ACAGACCCCCCACTGATCCACTTGGACTCCATCCCTGTCACTGACCCAGACCCAGCAGCT
GCTCCACCCGGCACAGGGGTGCCAGCCTGGGTGAGCAATGGGGATGCAGCGGACGCAGCC
TTCTCCGGGGCCCGGCACTCCAGCTGGAAGCGGAAGAGTTCCCGTCGCATTGACCGGTTC
ACTTTCCCCGCCCTGGAAGAGGATGTGATTTATGACGACGTCCCCTGCGAGAGCCCAGAT
GCGCATCAGCCCGGGGCAGAGAGGAACCTGCTCTACGAGGATGCGCACCGGGCTGGGGCC
CCTCGGCAGGCGGAGGACCTAGGCTGGAGCTCCAGTGAGTTCGAGAGCTACAGCGAGGAC
TCGGGGGAGGAGGCCAAGCCGGAGGTCGAGGTCGAGCCCGCCAAGCACCGAGTGTCCTTC
CAGCCCAAGATGACCCAGCTCATGAAGGCCGCCAAGAGCGGGACCAAGGATGGGCTGGAG
AAGACACGGATGGCCGTGATGCGCAAAGTCTCCTTCCTGCACAGGAAGGACGTCCTCGGT
GACTCGGAGGAGGAGGACATGGGGCTCCTGGAGGTCAGCGTTTCGGACATCAAGCCCCCA
GCCCCAGAGCTGGGCCCCATGCCAGAGGGCCTGAGCCCTCAGCAGGTGGTCCGGAGGCAT
ATCCTGGGCTCCATCGTGCAGAGCGAAGGCAGCTACGTGGAGTCTCTGAAGCGGATACTC
CAGGACTACCGCAACCCCCTGATGGAGATGGAGCCCAAGGCGCTGAGCGCCCGCAAGTGC
CAGGTGGTGTTCTTCCGCGTGAAGGAGATCCTGCACTGCCACTCCATGTTCCAGATCGCC
CTGTCCTCCCGCGTGGCTGAGTGGGATTCCACCGAGAAGATCGGGGACCTCTTCGTGGCC
TCGTTTTCCAAGTCCATGGTGCTAGATGTGTACAGTGACTACGTGAACAACTTCACCAGT
GCCATGTCCATCATCAAGAAGGCCTGCCTCACCAAGCCTGCCTTCCTCGAGTTCCTCAAG
CGACGGCAGGTGTGCAGCCCAGACCGTGTCACCCTCTACGGGCTGATGGTCAAGCCCATC
CAGAGGTTCCCACAGTTCATACTCCTGCTTCAGGACATGCTGAAGAACACCCCCAGGGGC
CATCCGGACAGGCTGTCGCTGCAGCTGGCCCTCACAGAGCTGGAGACGCTGGCTGAGAAG
CTGAACGAGCAGAAGCGGCTGGCTGACCAGGTGGCTGAGATCCAGCAGCTGACCAAGAGC
GTCAGTGACCGCAGCAGCCTCAACAAGCTGTTGACCTCAGGCCAGCGGCAGCTGCTCCTG
TGTGAGACGTTGACGGAGACCGTGTACGGTGACCGCGGGCAGCTAATTAAGTCCAAGGAG
CGTCGGGTCTTCCTGCTCAACGACATGCTTGTCTGTGCCAACATCAACTTCAAGCCTGCC
AACCACAGGGGCCAGCTGGAGATCAGCAGCCTGGTGCCCCTGGGGCCCAAGTATGTGGTG
AAGTGGAACACGGCGCTGCCCCAGGTGCAGGTGGTGGAGGTGGGCCAGGACGGTGGCACC
TATGACAAGGACAATGTGCTCATCCAGCACTCAGGCGCCAAGAAGGCCTCTGCCTCAGGG
CAGGCTCAGAATAAGGTGTACCTCGGCCCCCCACGCCTCTTCCAGGAGCTGCAGGACCTG
CAGAAGGACCTGGCCGTGGTGGAGCAGATCACGCTTCTCATCAGCACGCTGCACGGCACC
TACCAGAACCTGAACATGACTGTGGCTCAAGACTGGTGCCTGGCCCTGCAGAGGCTGATG
CGGGTGAAGGAGGAAGAGATCCACTCGGCCAACAAGTGCCGTCTCAGGCTCCTGCTTCCT
GGGAAACCCGACAAGTCCGGCCGCCCCATTAGCTTCATGGTGGTTTTCATCACCCCCAAC
CCCCTGAGCAAGATTTCCTGGGTCAACAGGTTACATTTGGCCAAAATCGGACTCCGGGAG
GAGAACCAGCCAGGCTGGCTATGCCCGGATGAGGACAAGAAGAGCAAAGCCCCATTCTGG
TGCCCGATCCTGGCCTGCTGCATCCCTGCCTTCTCCTCCCGGGCACTCAGCCTGCAGCTT
GGGGCCCTGGTCCACAGTCCTGTCAACTGTCCCCTGCTGGGTTTCTCAGCCGTCAGCACC
TCCCTTCCACAGGGCTACCTCTGGGTCGGGGGCGGACAGGAAGGCGCAGGGGGCCAGGTG
GAAATCTTTTCCTTGAACCGGCCCTCGCCCCGCACCGTCAAGTCCTTCCCACTGGCAGCC
CCTGTGCTCTGCATGGAGTATATCCCGGAGCTGGAGGAGGAGGCGGAGAGCAGAGACGAG
AGCCCGACAGTTGCTGACCCCTCGGCCACGGTGCATCCAACCATCTGCCTCGGGCTCCAG
GATGGCAGCATCCTCCTCTACAGCAGTGTGGACACTGGCACCCAGTGCCTGGTGAGCTGC
AGGAGCCCAGGTCTGCAGCCTGTGCTCTGCCTGCGACACAGCCCCTTCCACCTGCTCGCT
GGCCTGCAGGATGGGACCCTTGCTGCTTACCCTCGGACCAGCGGAGGTGTCCTGTGGGAC
CTGGAGAGCCCTCCCGTGTGCCTGACTGTGGGGCCCGGGCCTGTCCGCACCCTGTTGAGC
CTGGAGGATGCCGTGTGGGCCAGCTGTGGGCCCCGGGTCACTGTCCTGGAAGCCACCACC
CTGCAGCCTCAGCAAAGCTTCGAGGCGCACCAGGACGAGGCAGTGAGCGTGACACACATG
GTGAAGGCGGGCAGCGGCGTCTGGATGGCCTTCTCCTCCGGCACCTCCATCCGCCTCTTC
CACACTGAGACCCTGGAGCATCTGCAAGAGATCAACATCGCCACCAGGACCACCTTCCTC
CTGCCAGGCCAGAAGCACTTGTGTGTCACCAGCCTCCTGATCTGCCAGGGTCTGCTCTGG
GTGGGCACTGACCAGGGTGTCATCGTCCTGCTGCCCGTGCCTCGGCTGGAAGGCATCCCC
AAGATCACAGGGAAAGGCATGGTCTCACTCAATGGGCACTGTGGGCCTGTGGCCTTCCTG
GCTGTGGCTACCAGCATCCTGGCCCCTGACATCCTGCGGAGTGACCAGGAGGAGGCTGAG
GGGCCCCGGGCTGAGGAGGACAAGCCAGACGGGCAGGCACACGAGCCCATGCCCGACAGC
CACGTGGGCCGAGAGCTGACCCGCAAGAAGGGCATCCTCTTGCAGTACCGCCTGCGCTCC
ACCGCACACCTCCCGGGCCCGCTGCTCTCCATGCGGGAGCCGGCGCCTGCTGATGGCGCA
GCTTTGGAGCACAGCGAGGAGGACGGCTCCATTTACGAGATGGCCGACGACCCCGACGTC
TGGGTGCGCAGCCGGCCCTGCGCCCGCGACGCCCACCGCAAGGAGATTTGCTCTGTGGCC
ATCATCTCCGGCGGGCAGGGCTACCGCAACTTTGGCAGCGCTCTGGGCAGCAGTGGGAGG
CAGGCCCCGTGTGGGGAGACGGACAGCACCCTCCTCATCTGGCAGGTGCCCTTGATGCTA
Protein sequence predicted based on nucleotide sequence
MASSNPPPQPAIGDQLVPGVPGPSSEAEDDPGEAFEFDDSDDEEDTSAALGVPSLAPERD TDPPLIHLDSIPVTDPDPAAAPPGTGVPAWVSNGDAADAAFSGARHSSWKRKSSRRIDRF TFPALEEDVIYDDVPCESPDAHQPGAERNLLYEDAHRAGAPRQAEDLGWSSSEFESYSED SGEEAKPEVEVEPAKHRVSFQPKMTQLMKAAKSGTKDGLEKTRMAVMRKVSFLHRKDVLG DSEEEDMGLLEVSVSDIKPPAPELGPMPEGLSPQQVVRRHILGSIVQSEGSYVESLKRIL QDYRNPLMEMEPKALSARKCQVVFFRVKEILHCHSMFQIALSSRVAEWDSTEKIGDLFVA SFSKSMVLDVYSDYVNNFTSAMSIIKKACLTKPAFLEFLKRRQVCSPDRVTLYGLMVKPI QRFPQFILLLQDMLKNTPRGHPDRLSLQLALTELETLAEKLNEQKRLADQVAEIQQLTKS VSDRSSLNKLLTSGQRQLLLCETLTETVYGDRGQLIKSKERRVFLLNDMLVCANINFKPA NHRGQLEISSLVPLGPKYVVKWNTALPQVQVVEVGQDGGTYDKDNVLIQHSGAKKASASG QAQNKVYLGPPRLFQELQDLQKDLAVVEQITLLISTLHGTYQNLNMTVAQDWCLALQRLM RVKEEEIHSANKCRLRLLLPGKPDKSGRPISFMVVFITPNPLSKISWVNRLHLAKIGLRE ENQPGWLCPDEDKKSKAPFWCPILACCIPAFSSRALSLQLGALVHSPVNCPLLGFSAVST SLPQGYLWVGGGQEGAGGQVEIFSLNRPSPRTVKSFPLAAPVLCMEYIPELEEEAESRDE SPTVADPSATVHPTICLGLQDGSILLYSSVDTGTQCLVSCRSPGLQPVLCLRHSPFHLLA GLQDGTLAAYPRTSGGVLWDLESPPVCLTVGPGPVRTLLSLEDAVWASCGPRVTVLEATT LQPQQSFEAHQDEAVSVTHMVKAGSGVWMAFSSGTSIRLFHTETLEHLQEINIATRTTFL LPGQKHLCVTSLLICQGLLWVGTDQGVIVLLPVPRLEGIPKITGKGMVSLNGHCGPVAFL AVATSILAPDILRSDQEEAEGPRAEEDKPDGQAHEPMPDSHVGRELTRKKGILLQYRLRS TAHLPGPLLSMREPAPADGAALEHSEEDGSIYEMADDPDVWVRSRPCARDAHRKEICSVA IISGGQGYRNFGSALGSSGRQAPCGETDSTLLIWQVPLML
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
