Gene:
Gene Symbol:
LARP1
Gene Name:
La ribonucleoprotein domain family, member 1
Original Clone ID:
ccsbBroadEn_07871
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC001460.2
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00506007
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCTTTGGAGGGTGCTTTTGTCAAAGAGGCCTCCTTTCCCTCACCCAGAGCTGGATTTC
CAAGAGGCTCCCATACCTAGCTGCCCTGGCAGACTCCCAGGGAGGAAAAACAGCGTGGCC
TTGGCAGCTGCCCCGAGGAAGGAGCCCACAGGTGACAGGGAGAAGCCATTGCCATTCCCT
GTCCTGGCCCCCTTCAGCAACCCTGAACACTCTGCTCCAGCCAAGGTGGTGAGGGCAGCT
GTTCCTAAACAGCGCAAAGGCAGCAAGGTTGGTGACTTTGGAGATGCAATCAATTGGCCC
ACACCTGGAGAGATAGCCCACAAGAGTGTTCAGCCACAGTCCCACAAGCCTCAGCCTACC
CGTAAACTGCCACCCAAGAAGGACATGAAGGAACAGGAGAAAGGAGAAGGGAGTGATAGT
AAGGAGAGTCCAAAAACCAAATCAGATGAATCAGGGGAGGAAAAGAATGGAGATGAGGAT
TGCCAGCGAGGCGGGCAGAAGAAGAAAGGAAACAAACACAAGTGGGTTCCATTACAAATA
GACATGAAGCCTGAAGTGCCCAGAGAGAAACTGGCTTCACGCCCCACTCGCCCACCGGAG
CCTAGACACATACCTGCCAATCGCGGAGAGATCAAAGGGTCTGAGTCTGCCACCTACGTG
CCCGTGGCCCCCCCCACCCCAGCCTGGCAACCAGAGATCAAACCGGAGCCTGCCTGGCAC
GACCAGGATGAGACATCGAGTGTGAAGAGTGATGGGGCTGGTGGGGCGCGGGCTTCCTTC
CGTGGCCGTGGACGGGGGCGTGGTCGCGGCCGGGGACGCGGCCGGGGTGGCACTCGAACC
CATTTTGACTACCAGTTTGGCTACCGAAAGTTTGATGGTGTGGAGGGGCCTCGTACGCCC
AAGTACATGAACAACATCACCTACTACTTTGACAATGTCAGCAGCACCGAGCTTTACAGT
GTGGATCAGGAACTGCTCAAAGACTACATCAAGCGCCAGATTGAATACTACTTCAGCGTG
GACAATTTAGAGCGAGACTTCTTCCTGCGAAGGAAAATGGATGCTGATGGTTTCCTACCC
ATCACCCTTATTGCTTCCTTCCACCGAGTGCAGGCCCTTACCACTGACATTTCACTCATC
TTTGCGGCCCTAAAGGACAGCAAGGTGGTGGAGATCGTTGATGAGAAAGTTCGTAGGAGG
GAGGAACCAGAAAAGTGGCCTCTTCCCCCAATAGTGGATTATTCACAGACTGATTTCTCC
CAGCTTCTCAACTGCCCTGAATTTGTTCCCCGTCAGCACTACCAAAAGGAGACAGAGTCG
GCACCTGGCTCTCCTCGTGCAGTCACCCCAGTGCCAACCAAAACAGAGGAGGTCAGCAAC
CTAAAGACACTACCCAAGGGCCTGTCTGCCAGCCTGCCTGACCTGGATTCTGAGAACTGG
ATTGAAGTGAAGAAGAGGCCTCGGCCATCCCCAGCACGGCCCAAGAAGTCAGAGGAGTCC
AGATTTTCCCACCTGACCTCCCTGCCTCAGCAGCTGCCTTCCCAGCAGCTGATGTCCAAG
GATCAGGATGAGCAAGAGGAACTGGATTTTCTGTTTGACGAGGAGATGGAGCAGATGGAT
GGGCGGAAGAACACCTTCACTGCCTGGTCTGATGAGGAATCTGACTATGAGATTGATGAC
AGGGATGTCAACAAGATCCTCATTGTCACCCAGACACCACATTACATGCGCCGGCACCCA
GGGGGGGACCGCACAGGCAACCACACCTCGCGTGCCAAGATGAGCGCCGAACTGGCCAAG
GTCATTAATGATGGCCTCTTCTACTATGAGCAGGACCTGTGGGCTGAAAAGTTTGAACCT
GAGTATTCCCAGATCAAGCAAGAAGTCGAGAACTTCAAAAAGGTCAATATGATCAGCCGG
GAGCAGTTTGACACACTGACCCCTGAGCCCCCTGTGGATCCCAACCAGGAAGTTCCTCCT
GGGCCACCTCGGTTCCAGCAAGTTCCTACGGATGCCCTGGCCAACAAGTTGTTTGGTGCT
CCTGAGCCCTCCACCATCGCCCGCTCTCTACCAACCACTGTCCCAGAGTCACCAAACTAC
CGCAACACCAGGACCCCTCGCACTCCCCGGACACCACAGCTCAAAGACTCAAGCCAGACA
TCACGGTTTTACCCAGTGGTGAAAGAAGGACGGACACTGGATGCCAAGATGCCTCGAAAA
AGAAAGACAAGACACAGTTCAAACCCACCCTTGGAGAGCCATGTGGGCTGGGTGATGGAT
TCCCGTGAGCACAGGCCCCGTACTGCTTCCATCAGCTCCAGCCCCTCAGAAGGGACGCCT
ACAGTTGGCAGCTATGGCTGTACCCCTCAGTCATTGCCCAAGTTCCAGCATCCTTCCCAT
GAACTGCTCAAGGAAAATGGCTTCACACAACACGTCTACCATAAGTATCGTAGGCGCTGC
CTTAATGAGCGGAAACGCTTGGGCATTGGCCAGTCTCAGGAGATGAACACACTCTTCCGC
TTCTGGTCCTTCTTCCTCCGAGATCACTTCAACAAAAAGATGTATGAGGAGTTCAAGCAG
CTGGCTCTGGAGGACGCCAAAGAAGGCTACAGATATGGTTTGGAGTGCCTTTTTCGATAC
TACAGTTATGGCCTGGAAAAGAAGTTCCGGCTGGACATATTCAAGGATTTTCAGGAGGAA
ACGGTGAAGGACTATGAAGCTGGCCAACTGTATGGGCTGGAGAAGTTCTGGGCCTTCTTG
AAATATTCCAAAGCCAAAAATTTGGACATTGACCCCAAACTGCAAGAATACCTCGGCAAA
TTCCGACGTCTTGAAGACTTCCGAGTAGATCCCCCCATGGGTGAGGAGGGCAACCACAAG
CGACACTCAGTGGTAGCAGGAGGTGGCGGCGGTGAGGGCAGGAAGCGGTGCCCCTCCCAG
TCTTCCAGCAGGCCTGCTGCCATGATCAGCCAACCCCCTACACCACCCACCGGCCAGCCT
GTCCGGGAAGATGCCAAATGGACAAGCCAGCACTCGAACACACAGACTTTGGGAAAG
Protein sequence predicted based on nucleotide sequence
MLWRVLLSKRPPFPHPELDFQEAPIPSCPGRLPGRKNSVALAAAPRKEPTGDREKPLPFP VLAPFSNPEHSAPAKVVRAAVPKQRKGSKVGDFGDAINWPTPGEIAHKSVQPQSHKPQPT RKLPPKKDMKEQEKGEGSDSKESPKTKSDESGEEKNGDEDCQRGGQKKKGNKHKWVPLQI DMKPEVPREKLASRPTRPPEPRHIPANRGEIKGSESATYVPVAPPTPAWQPEIKPEPAWH DQDETSSVKSDGAGGARASFRGRGRGRGRGRGRGRGGTRTHFDYQFGYRKFDGVEGPRTP KYMNNITYYFDNVSSTELYSVDQELLKDYIKRQIEYYFSVDNLERDFFLRRKMDADGFLP ITLIASFHRVQALTTDISLIFAALKDSKVVEIVDEKVRRREEPEKWPLPPIVDYSQTDFS QLLNCPEFVPRQHYQKETESAPGSPRAVTPVPTKTEEVSNLKTLPKGLSASLPDLDSENW IEVKKRPRPSPARPKKSEESRFSHLTSLPQQLPSQQLMSKDQDEQEELDFLFDEEMEQMD GRKNTFTAWSDEESDYEIDDRDVNKILIVTQTPHYMRRHPGGDRTGNHTSRAKMSAELAK VINDGLFYYEQDLWAEKFEPEYSQIKQEVENFKKVNMISREQFDTLTPEPPVDPNQEVPP GPPRFQQVPTDALANKLFGAPEPSTIARSLPTTVPESPNYRNTRTPRTPRTPQLKDSSQT SRFYPVVKEGRTLDAKMPRKRKTRHSSNPPLESHVGWVMDSREHRPRTASISSSPSEGTP TVGSYGCTPQSLPKFQHPSHELLKENGFTQHVYHKYRRRCLNERKRLGIGQSQEMNTLFR FWSFFLRDHFNKKMYEEFKQLALEDAKEGYRYGLECLFRYYSYGLEKKFRLDIFKDFQEE TVKDYEAGQLYGLEKFWAFLKYSKAKNLDIDPKLQEYLGKFRRLEDFRVDPPMGEEGNHK RHSVVAGGGGGEGRKRCPSQSSSRPAAMISQPPTPPTGQPVREDAKWTSQHSNTQTLGK
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
