PDZD8 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  PDZD8
Gene Name:  PDZ domain containing 8
Original Clone ID:
ccsbBroadEn_04735
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC028375.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00506009
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3463nts
Open reading frame: 1 to 3463

ATGGGGCTGCTGCTCATGATCCTGGCGTCGGCCGTGCTGGGTTCCTTCCTCACGCTCCTC
GCCCAGTTCTTCCTGCTGTACCGCAGACAGCCCGAGCCGCCGGCGGACGAGGCCGCCCGC
GCGGGCGAGGGCTTCCGCTACATCAAGCCAGTGCCGGGCCTGCTCCTAAGGGAGTACCTT
TATGGCGGCGGCCGGGATGAGGAGCCCTCCGGAGCGGCCCCTGAGGGCGGCGCGACCCCC
ACCGCGGCCCCCGAGACCCCCGCCCCGCCGACGCGGGAGACTTGCTACTTCCTCAACGCC
ACCATCCTATTCCTGTTCCGGGAGTTGCGGGACACCGCGCTGACCCGCCGCTGGGTCACC
AAGAAGATCAAGGTGGAGTTCGAGGAGCTGCTGCAGACCAAGACGGCCGGGCGCCTGCTG
GAGGGGCTGAGCCTGCGGGACGTGTTCCTGGGCGAGACGGTGCCCTTCATCAAGACCATC
CGGCTCGTGCGGCCAGTCGTGCCCTCGGCCACCGGGGAGCCCGATGGCCCTGAAGGGGAG
GCGCTGCCCGCCGCCTGCCCCGAGGAGCTGGCCTTCGAGGCGGAGGTGGAGTACAACGGG
GGCTTCCACCTGGCCATCGACGTGGACCTGGTCTTCGGCAAGTCCGCCTACTTGTTTGTC
AAGCTGTCCCGCGTGGTGGGAAGGCTGCGCTTGGTCTTTACGCGCGTGCCCTTCACCCAC
TGGTTCTTCTCCTTCGTGGAAGACCCGCTGATCGACTTCGAGGTGCGCTCCCAGTTTGAA
GGGCGGCCCATGCCCCAGCTCACCTCCATCATCGTCAACCAGCTCAAGAAGATCATCAAG
CGCAAGCACACCCTACCGAATTACAAGATCAGGTTTAAGCCGTTTTTTCCATACCAGACC
TTGCAAGGATTTGAAGAAGATGAAGAGCATATCCATATACAACAATGGGCACTTACTGAA
GGCCGTCTTAAAGTTACGTTGTTAGAATGTAGCAGGTTACTCATTTTTGGATCCTATGAC
AGAGAGGCAAATGTTCATTGTACACTTGAGTTAAGCAGTAGTGTTTGGGAAGAAAAACAG
AGGAGTTCTATTAAGACGGTTGAATTAATAAAAGGAAATTTACAAAGTGTTGGACTTACA
CTTCGTCTTGTCCAGTCAACTGATGGGTATGCTGGGCACGTCATCATTGAAACTGTGGCT
CCAAACTCGCCTGCTGCAATTGCAGATCTTCAGCGGGGAGATCGACTTATCGCCATTGGA
GGTGTGAAAATCACATCAACACTGCAAGTGTTGAAGCTTATCAAGCAGGCTGGTGACCGA
GTCCTGGTGTACTATGAAAGGCCTGTTGGCCAGAGTAATCAAGGTGCAGTGCTGCAAGAT
AACTTTGGCCAGTTGGAAGAAAACTTTTTGTCAAGCTCATGCCAATCGGGTTATGAAGAG
GAAGCTGCCGGGTTGACAGTAGATACTGAAAGTAGAGAGCTGGATTCTGAATTTGAAGAC
TTGGCAAGTGATGTCAGAGCACAAAATGAGTTCAAAGATGAGGCACAATCATTAAGTCAT
AGTCCCAAACGTGTTCCAACAACACTTTCTATTAAACCCCTTGGAGCTATATCACCAGTT
TTAAACCGTAAATTAGCTGTAGGAAGTCACCCACTACCACCGAAAATTCAGTCCAAAGAT
GGAAATAAACCTCCACCCCTAAAAACTTCTGAGATAACAGACCCAGCACAAGTGTCAAAA
CCAACCCAAGGATCTGCTTTCAAACCACCTGTGCCACCACGACCACAAGCGAAAGTTCCT
TTGCCTTCCGCCGATGCTCCAAATCAGGCAGAACCAGATGTTCTCGTTGAAAAGCCAGAG
AAGGTGGTGCCACCTCCTCTTGTAGATAAATCTGCTGAAAAGCAAGCAAAAAATGTGGAT
GCCATAGACGATGCAGCTGCACCTAAGCAATTTTTAGCAAAGCAAGAAGTGGCCAAAGAT
GTCACTTCAGAAACTTCCTGCCCTACTAAGGACAGTTCGGACGACCGTCAAACATGGGAA
TCATCAGAAATTCTTTATCGTAATAAGCTAGGAAAATGGACAAGAACCAGAGCATCCTGT
TTGTTTGACATAGAAGCCTGTCACAGGTACTTAAACATTGCATTGTGGTGCAGGGATCCT
TTCAAGTTGGGAGGTCTCATCTGTTTGGGGCATGTTAGTTTAAAACTTGAAGATGTGGCT
TTAGGATGCCTAGCTACATCAAACACGGAATACCTTTCCAAATTGAGACTGGAAGCCCCC
TCACCTAAGGCTATAGTCACTAGAACCGCACTACGCAATCTGAGTATGCAAAAGGGATTC
AATGACAAATTTTGCTATGGTGACATTACTATTCACTTCAAATATTTGAAAGAAGGAGAA
TCAGACCACCATGTAGTTACTAACGTAGAAAAAGAAAAAGAACCCCATTTGGTTGAAGAA
GTTTCTGTTCTCCCTAAAGAGGAGCAATTTGTTGGACAGATGGGTTTAACAGAAAACAAA
CACAGTTTTCAGGATACTCAGTTCCAGAACCCAACATGGTGTGACTACTGTAAGAAAAAA
GTTTGGACTAAAGCAGCTTCCCAGTGTATGTTTTGTGCTTATGTTTGCCATAAAAAATGT
CAAGAAAAGTGTCTAGCTGAGACTTCTGTTTGTGGAGCAACTGATAGGCGAATAGACAGG
ACACTGAAAAACCTTAGGCTGGAAGGACAGGAAACCCTCTTAGGCCTGCCTCCTCGTGTT
GATGCTGAAGCTAGCAAGTCAGTCAATAAAACAACAGGTTTGACAAGGCATATTATCAAT
ACTAGTTCTCGTTTATTAAATTTGCGTCAAGTCTCTAAAACTCGCCTTTCTGAACCAGGA
ACCGATCTCGTAGAACCTTCACCAAAACACACACCCAACACGTCAGACAACGAAGGCAGT
GACACGGAGGTCTGTGGTCCAAACAGTCCTTCTAAACGGGGAAACAGCACAGGAATAAAG
TTAGTGAGAAAAGAGGGTGGTCTGGATGACAGTGTTTTCATTGCAGTTAAAGAAATTGGT
CGTGATCTGTACAGGGGCTTGCCTACAGAGGAAAGGATCCAGAAACTAGAGTTCATGTTG
GATAAGCTACAGAATGAAATTGATCAGGAGTTGGAACACAATAATTCCCTTGTTAGAGAA
GAAAAAGAGACAACTGATACAAGGAAAAAATCACTTCTTTCTGCTGCCTTAGCTAAATCA
GGTGAAAGGCTACAAGCTCTAACACTTCTTATGATTCACTACAGAGCAGGCATTGAAGAT
ATAGAAACTTTAGAAAGTCTGTCTTTAGACCAGCACTCCAAAAAAATAAGCAAGTACACA
GATGATACAGAAGAAGACCTTGATAATGAAATAAGCCAACTAATAGACTCTCAGCCATTC
AGCAGCATATCAGATGACTTATTTGGCCCATCCGAGTCTGTG

Protein sequence predicted based on nucleotide sequence

MGLLLMILASAVLGSFLTLLAQFFLLYRRQPEPPADEAARAGEGFRYIKPVPGLLLREYL
YGGGRDEEPSGAAPEGGATPTAAPETPAPPTRETCYFLNATILFLFRELRDTALTRRWVT
KKIKVEFEELLQTKTAGRLLEGLSLRDVFLGETVPFIKTIRLVRPVVPSATGEPDGPEGE
ALPAACPEELAFEAEVEYNGGFHLAIDVDLVFGKSAYLFVKLSRVVGRLRLVFTRVPFTH
WFFSFVEDPLIDFEVRSQFEGRPMPQLTSIIVNQLKKIIKRKHTLPNYKIRFKPFFPYQT
LQGFEEDEEHIHIQQWALTEGRLKVTLLECSRLLIFGSYDREANVHCTLELSSSVWEEKQ
RSSIKTVELIKGNLQSVGLTLRLVQSTDGYAGHVIIETVAPNSPAAIADLQRGDRLIAIG
GVKITSTLQVLKLIKQAGDRVLVYYERPVGQSNQGAVLQDNFGQLEENFLSSSCQSGYEE
EAAGLTVDTESRELDSEFEDLASDVRAQNEFKDEAQSLSHSPKRVPTTLSIKPLGAISPV
LNRKLAVGSHPLPPKIQSKDGNKPPPLKTSEITDPAQVSKPTQGSAFKPPVPPRPQAKVP
LPSADAPNQAEPDVLVEKPEKVVPPPLVDKSAEKQAKNVDAIDDAAAPKQFLAKQEVAKD
VTSETSCPTKDSSDDRQTWESSEILYRNKLGKWTRTRASCLFDIEACHRYLNIALWCRDP
FKLGGLICLGHVSLKLEDVALGCLATSNTEYLSKLRLEAPSPKAIVTRTALRNLSMQKGF
NDKFCYGDITIHFKYLKEGESDHHVVTNVEKEKEPHLVEEVSVLPKEEQFVGQMGLTENK
HSFQDTQFQNPTWCDYCKKKVWTKAASQCMFCAYVCHKKCQEKCLAETSVCGATDRRIDR
TLKNLRLEGQETLLGLPPRVDAEASKSVNKTTGLTRHIINTSSRLLNLRQVSKTRLSEPG
TDLVEPSPKHTPNTSDNEGSDTEVCGPNSPSKRGNSTGIKLVRKEGGLDDSVFIAVKEIG
RDLYRGLPTEERIQKLEFMLDKLQNEIDQELEHNNSLVREEKETTDTRKKSLLSAALAKS
GERLQALTLLMIHYRAGIEDIETLESLSLDQHSKKISKYTDDTEEDLDNEISQLIDSQPF
SSISDDLFGPSESV

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable