Gene:
Gene Symbol:
TONSL
Gene Name:
tonsoku-like, DNA repair protein
Original Clone ID:
ccsbBroadEn_10997
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC008782.2
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00506011
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAGGACCCGCCTCTATCTCAACCTGGGCCTCACCTTTGAGAGCCTGCAGCAGACAGCC
CTGTGCAACGATTACTTCAGGAAGAGCATCTTCCTTGCGGAGCAGAACCACCTTTACGAG
GACCTATTCCGCGCCCGCTACAACCTGGGCACCATCCACTGGCGCGCGGGCCAGCACTCC
CAGGCTATGCGCTGCTTGGAGGGTGCCCGGGAGTGTGCGCACACCATGAGGAAGCGGTTC
ATGGAGAGCGAGTGCTGCGTGGTTATTGCACAGGTCCTCCAAGACCTGGGAGACTTTTTG
GCTGCCAAGCGAGCCCTGAAGAAGGCCTACAGGCTGGGCTCCCAGAAGCCTGTGCAGAGG
GCAGCCATCTGTCAGAACCTCCAGCATGTGCTGGCAGTGGTCCGGCTGCAGCAACAGCTG
GAAGAGGCTGAGGGCAGAGACCCTCAGGGTGCCATGGTCATCTGTGAGCAGCTAGGGGAC
CTCTTCTCCAAGGCAGGAGACTTTCCCAGGGCAGCTGAGGCTTACCAGAAGCAGCTGCGT
TTTGCTGAGCTGCTGGACAGACCGGGTGCTGAGCGGGCCATCATCCACGTGTCCCTGGCC
ACCACACTGGGAGACATGAAGGACCACCATGGGGCCGTGCGCCACTATGAGGAGGAACTG
AGGCTGCGCAGCGGCAACGTGCTGGAGGAGGCCAAGACCTGGCTGAACATTGCACTGTCC
CGCGAGGAGGCCGGCGATGCCTACGAGCTGCTGGCCCCGTGCTTCCAGAAAGCGCTCAGC
TGTGCCCAGCAGGCCCAGCGTCCCCAGCTGCAGAGGCAGGTCTTGCAGCATCTCCATACC
GTGCAGCTGAGGCTGCAGCCCCAGGAGGCCCCTGAGACCGAAACCAGACTGCGGGAGCTC
AGTGTAGCTGAAGATGAAGATGAGGAGGAGGAGGCGGAGGAGGCGGCAGCCACAGCGGAG
AGCGAAGCCCTGGAGGCCGGCGAGGTGGAGCTCTCAGAGGGCGAGGACGACACCGATGGC
CTGACCCCGCAGCTGGAGGAGGACGAGGAGCTTCAGGGCCACCTGGGCCGGCGGAAGGGG
AGCAAGTGGAACCGGCGAAACGACATGGGGGAGACCCTGCTGCACCGAGCCTGCATCGAG
GGCCAGCTGCGCCGCGTCCAGGACCTTGTGAGGCAGGGCCACCCCCTTAACCCTCGGGAC
TACTGTGGCTGGACACCTCTGCACGAGGCCTGCAACTACGGGCATCTAGAAATTGTCCGC
TTCCTGCTGGACCACGGGGCCGCAGTGGACGACCCAGGTGGCCAGGGCTGCGAAGGCATC
ACCCCCCTCCACGATGCCCTCAACTGTGGCCACTTCGAGGTGGCTGAGCTGCTGCTTGAA
CGGGGGGCGTCCGTCACCCTCCGCACTCGAAAGGGCCTCAGCCCGCTGGAGACGCTGCAG
CAGTGGGTGAAGCTGTACCGCAGGGACCTGGACCTGGAGACGCGGCAGAAGGCCAGGGCC
ATGGAGATGCTGCTCCAGGCGGCTGCCTCGGGCCAAGATCCCCACAGCTCCCAGGCCTTC
CACACCCCAAGCAGCCTTCTGTTTGACCCCGAGACCTCTCCTCCTTTGAGCCCCTGCCCA
GAACCCCCCTCTAATAGCACTAGACTCCCAGAGGCCTCTCAGGTCCATGTCAGGGTCTCC
CCAGGGCAGGCGGCACCAGCCATGGCCAGGCCTCGGAGGAGCAGGCATGGGCCAGCCAGC
AGCAGCAGCAGCTCAGAAGGCGAGGACAGCGCAGGCCCCGCACGGCCGTCCCAGAAGAGG
CCTCGGTGCTCGGCCACAGCACAACGGGTGGCAGCCTGGACGCCTGGCCCCGCCAGCAAC
AGGGAAGCAGCCACAGCCAGCACCAGCCGGGCAGCCTACCAGGCAGCCATCCGGGGTGTG
GGCAGTGCTCAGAGCCGGCTGGGGCCTGGCCCACCGCGGGGCCACAGCAAAGCCCTTGCC
CCCCAGGCAGCGCTCATCCCGGAGGAGGAGTGCCTGGCTGGGGACTGGCTGGAGCTGGAC
ATGCCCCTGACCCGCAGCCGCCGGCCCCGCCCCCGGGGCACTGGAGACAACCGCAGGCCC
AGTAGTACCTCTGGGTCGGACAGTGAGGAGAGCAGGCCCCGTGCCCGAGCCAAGCAGGTC
CGCCTGACCTGCATGCAGAGTTGCAGTGCGCCAGTTAACGCAGGGCCCAGCAGCCTGGCT
TCAGAACCTCCAGGGAGCCCCAGCACCCCCAGGGTCTCAGAGCCCAGTGGGGACAGCTCT
GCGGCAGGCCAGCCCTTGGGTCCGGCCCCGCCCCCTCCCATCCGGGTTCGAGTTCAAGTT
CAGGATCATCTCTTCCTCATCCCTGTCCCACACAGCAGTGACACCCACTCTGTGGCCTGG
CTGGCCGAGCAGGCGGCCCAGCGCTACTACCAGACCTGCGGGCTGCTGCCCAGGCTCACC
CTACGGAAAGAGGGGGCCCTGCTGGCCCCACAGGACCTCATCCCTGATGTGCTGCAGAGC
AATGACGAGGTGTTGGCTGAGGTGACTTCGTGGGACCTGCCCCCGTTGACTGACCGCTAC
CGCAGGGCCTGCCAGAGCCTGGGGCAAGGGGAGCACCAACAGGTGCTGCAGGCCGTGGAG
CTCCAGGGCTTGGGCCTCTCGTTCAGCGCCTGCTCCCTGGCCCTGGACCAGGCCCAGCTT
ACACCCCTGCTGCGGGCCCTCAAGCTGCACACAGCACTCCGGGAGCTGCGCCTGGCAGGG
AACCGGCTGGGGGACAAGTGTGTGGCTGAGCTGGTGGCTGCCCTGGGCACCATGCCCAGC
CTGGCCCTCCTTGACCTCTCCTCCAATCACCTGGGTCCCGAAGGCCTGCGCCAGCTTGCC
ATGGGGCTCCCAGGCCAAGCCACCTTGCAGAGTTTGGAGGAGCTGGACTTAAGCATGAAC
CCCCTGGGGGACGGCTGTGGCCAGTCCCTGGCCTCCCTCCTGCACGCCTGCCCCTTACTC
AGCACCCTGCGCCTGCAGGCGTGTGGCTTCGGCCCCAGCTTCTTTCTGAGCCACCAGACA
GCACTGGGTAGTGCTTTCCAAGATGCTGAGCACCTGAAGACCCTGTCCCTGTCCTACAAC
GCCCTGGGAGCCCCTGCCCTGGCCAGGACCCTGCAGAGCCTGCCCGCCGGCACCCTCCTG
CACTTAGAGCTCAGCTCCGTGGCAGCCGGCAAGGGTGATTCGGACCTCATGGAGCCTGTA
TTCCGATACCTGGCCAAGGAAGGCTGTGCTCTAGCCCACCTGACCCTGTCTGCAAACCAC
CTGGGGGACAAGGCTGTTAGAGACCTGTGCAGATGTCTCTCTCTGTGCCCCTCACTCATC
TCACTGGATCTGTCTGCCAACCCTGAGATCAGCTGTGCCAGCTTGGAAGAGCTCCTGTCC
ACCCTCCAAAAGCGGCCCCAAGGCCTTAGCTTCCTTGGCCTGTCAGGCTGCGCCGTCCAG
GGTCCCCTGGGCCTGGGCCTGTGGGACAAGATAGCCGCGCAGCTCCGGGAACTGCAGCTG
TGCAGCAGACGCCTCTGCGCTGAGGACAGGGACGCCCTGCGCCAGCTGCAGCCCAGTCGG
CCGGGCCCCGGCGAGTGCACGCTGGACCACGGCTCCAAGCTCTTCTTTCGGCGCCTC
Protein sequence predicted based on nucleotide sequence
MRTRLYLNLGLTFESLQQTALCNDYFRKSIFLAEQNHLYEDLFRARYNLGTIHWRAGQHS QAMRCLEGARECAHTMRKRFMESECCVVIAQVLQDLGDFLAAKRALKKAYRLGSQKPVQR AAICQNLQHVLAVVRLQQQLEEAEGRDPQGAMVICEQLGDLFSKAGDFPRAAEAYQKQLR FAELLDRPGAERAIIHVSLATTLGDMKDHHGAVRHYEEELRLRSGNVLEEAKTWLNIALS REEAGDAYELLAPCFQKALSCAQQAQRPQLQRQVLQHLHTVQLRLQPQEAPETETRLREL SVAEDEDEEEEAEEAAATAESEALEAGEVELSEGEDDTDGLTPQLEEDEELQGHLGRRKG SKWNRRNDMGETLLHRACIEGQLRRVQDLVRQGHPLNPRDYCGWTPLHEACNYGHLEIVR FLLDHGAAVDDPGGQGCEGITPLHDALNCGHFEVAELLLERGASVTLRTRKGLSPLETLQ QWVKLYRRDLDLETRQKARAMEMLLQAAASGQDPHSSQAFHTPSSLLFDPETSPPLSPCP EPPSNSTRLPEASQVHVRVSPGQAAPAMARPRRSRHGPASSSSSSEGEDSAGPARPSQKR PRCSATAQRVAAWTPGPASNREAATASTSRAAYQAAIRGVGSAQSRLGPGPPRGHSKALA PQAALIPEEECLAGDWLELDMPLTRSRRPRPRGTGDNRRPSSTSGSDSEESRPRARAKQV RLTCMQSCSAPVNAGPSSLASEPPGSPSTPRVSEPSGDSSAAGQPLGPAPPPPIRVRVQV QDHLFLIPVPHSSDTHSVAWLAEQAAQRYYQTCGLLPRLTLRKEGALLAPQDLIPDVLQS NDEVLAEVTSWDLPPLTDRYRRACQSLGQGEHQQVLQAVELQGLGLSFSACSLALDQAQL TPLLRALKLHTALRELRLAGNRLGDKCVAELVAALGTMPSLALLDLSSNHLGPEGLRQLA MGLPGQATLQSLEELDLSMNPLGDGCGQSLASLLHACPLLSTLRLQACGFGPSFFLSHQT ALGSAFQDAEHLKTLSLSYNALGAPALARTLQSLPAGTLLHLELSSVAAGKGDSDLMEPV FRYLAKEGCALAHLTLSANHLGDKAVRDLCRCLSLCPSLISLDLSANPEISCASLEELLS TLQKRPQGLSFLGLSGCAVQGPLGLGLWDKIAAQLRELQLCSRRLCAEDRDALRQLQPSR PGPGECTLDHGSKLFFRRL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
