Gene:
Gene Symbol:
DHX38
Gene Name:
DEAH (Asp-Glu-Ala-His) box polypeptide 38
Original Clone ID:
ccsbBroadEn_07485
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC004235.2
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00506014
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGGGGACACCAGTGAGGATGCCTCGATCCATCGATTGGAAGGCACTGATCTGGACTGT
CAGGTTGGCGGTCTTATTTGCAAGTCCAAAAGTGCGGCCAGCGAGCAGCATGTCTTCAAG
GCTCCTGCTCCCCGCCCTTCATTACTGGGACTGGACTTGCTGGCTTCCCTGAAACGGAGA
GAGCGAGAGGAGAAGGACGATGGGGAGGACAAGAAGAAGTCCAAAGTCTCCTCCTACAAG
GACTGGGAAGAGAGCAAGGATGACCAGAAGGATGCTGAGGAAGAGGGCGGTGACCAGGCT
GGCCAAAATATCCGGAAAGACAGACATTATCGGTCTGCTCGGGTAGAGACTCCATCCCAT
CCGGGTGGTGTGAGCGAAGAGTTTTGGGAACGCAGTCGGCAGAGAGAGCGGGAGCGGCGG
GAACATGGTGTCTATGCCTCGTCCAAAGAAGAAAAGGATTGGAAGAAGGAGAAATCGCGG
GATCGAGACTATGACCGCAAGAGGGACAGAGATGAGCGGGATAGAAGTAGGCACAGCAGC
AGATCAGAGCGAGATGGAGGGTCAGAGCGTAGCAGCAGAAGAAATGAACCCGAGAGCCCA
CGACATCGACCTAAAGATGCAGCCACCCCTTCAAGGTCTACCTGGGAGGAAGAGGACAGT
GGCTATGGCTCCTCAAGGCGCTCACAGTGGGAATCGCCCTCCCCGACGCCTTCCTATCGG
GATTCTGAGCGGAGCCATCGGCTGTCCACTCGAGATCGAGACAGGTCTGTGAGGGGCAAG
TACTCGGATGACACGCCTCTGCCAACTCCCTCCTACAAATATAACGAGTGGGCCGATGAC
AGAAGACACTTGGGGTCCACCCCGCGTCTGTCCAGGGGCCGAGGAAGACGTGAGGAGGGC
GAAGAAGGAATTTCATTTGACACGGAGGAGGAGCGGCAGCAGTGGGAAGATGACCAGAGG
CAAGCCGATCGGGATTGGTACATGATGGACGAGGGCTATGACGAGTTCCACAACCCGCTG
GCCTACTCCTCCGAGGACTACGTGAGGAGGCGGGAGCAGCACCTGCATAAACAGAAGCAG
AAGCGCATTTCAGCTCAGCGGAGACAGATCAATGAGGATAACGAGCGCTGGGAGACAAAC
CGCATGCTCACCAGTGGGGTGGTCCATCGGCTGGAGGTGGATGAGGACTTTGAAGAGGAC
AACGCGGCCAAGGTGCATCTGATGGTGCACAATCTGGTGCCTCCCTTTCTGGATGGGCGC
ATTGTCTTCACCAAGCAGCCGGAGCCGGTGATTCCAGTGAAGGATGCCACTTCTGACCTG
GCCATCATTGCTCGGAAAGGCAGCCAGACAGTGCGGAAGCACAGGGAGCAGAAGGAGCGC
AAGAAGGCTCAGCACAAACACTGGGAACTGGCGGGGACCAAACTGGGAGATATAATGGGC
GTCAAGAAGGAGGAAGAGCCAGATAAAGCTGTGACGGAGGATGGGAAGGTGGACTACAGG
ACAGAGCAGAAGTTTGCAGATCACATGAAGAGAAAGAGCGAAGCCAGCAGTGAATTTGCA
AAGAAGAAGTCCATCCTGGAGCAGAGGCAGTACCTGCCCATCTTTGCAGTGCAGCAGGAG
CTGCTCACTATTATCAGAGACAACAGCATCGTGATCGTGGTTGGGGAGACGGGGAGTGGT
AAGACCACTCAGCTGACGCAGTACCTGCATGAAGATGGTTACACGGACTATGGGATGATT
GGGTGTACCCAGCCCCGGCGTGTAGCTGCCATGTCAGTGGCCAAGAGAGTCAGTGAAGAG
ATGGGGGGAAACCTTGGCGAGGAGGTGGGCTATGCCATCCGCTTTGAAGACTGCACTTCA
GAGAACACCTTGATCAAATACATGACTGACGGGATCCTGCTCCGAGAGTCCCTCCGGGAA
GCCGACCTGGATCACTACAGTGCCATCATCATGGACGAGGCCCACGAGCGCTCCCTCAAC
ACTGACGTGCTCTTTGGGCTGCTCCGGGAGGTAGTGGCTCGGCGCTCAGACCTGAAGCTC
ATCGTCACATCAGCCACGATGGATGCGGAGAAGTTTGCTGCCTTTTTTGGGAATGTCCCC
ATCTTCCACATCCCTGGCCGTACCTTCCCTGTTGACATCCTCTTCAGCAAGACCCCACAG
GAGGATTACGTGGAGGCTGCAGTGAAGCAGTCCTTGCAGGTGCACCTGTCGGGGGCCCCT
GGAGACATCCTTATCTTCATGCCTGGCCAAGAGGACATTGAGGTGACCTCAGACCAGATT
GTGGAGCATCTGGAGGAACTGGAGAACGCGCCTGCCCTGGCTGTGCTGCCCATCTACTCT
CAGCTGCCTTCTGACCTCCAGGCCAAAATCTTCCAGAAGGCTCCAGATGGCGTTCGGAAG
TGCATCGTTGCCACCAATATTGCCGAGACGTCTCTCACTGTTGACGGCATCATGTTTGTT
ATCGATTCTGGTTATTGCAAATTAAAGGTCTTCAACCCCAGGATTGGCATGGATGCTCTG
CAGATCTATCCCATTAGCCAGGCCAATGCCAACCAGCGGTCAGGGCGAGCCGGCAGGACG
GGCCCAGGTCAGTGTTTCAGGCTCTACACCCAGAGCGCCTACAAGAATGAGCTCCTGACC
ACCACAGTGCCCGAGATCCAGAGGACTAACCTGGCCAACGTGGTGCTGCTGCTCAAGTCC
CTCGGGGTGCAGGACCTGCTGCAGTTCCACTTCATGGACCCGCCCCCGGAGGACAACATG
CTCAACTCTATGTATCAGCTCTGGATCCTCGGGGCCCTGGACAACACAGGTGGTCTGACC
TCTACCGGGCGGCTGATGGTGGAGTTCCCGCTGGACCCTGCCCTGTCCAAGATGCTCATC
GTGTCCTGTGACATGGGCTGCAGCTCCGAGATCCTGCTCATCGTTTCCATGCTCTCGGTC
CCAGCCATCTTCTACAGGCCCAAGGGTCGAGAGGAGGAGAGTGATCAAATCCGGGAGAAG
TTCGCTGTTCCTGAGAGCGATCATTTGACCTACCTGAATGTTTACCTGCAGTGGAAGAAC
AATAATTACTCCACCATCTGGTGTAACGATCATTTCATCCATGCTAAGGCCATGCGGAAG
GTCCGGGAGGTGCGAGCTCAACTCAAGGACATCATGGTGCAGCAGCGGATGAGCCTGGCC
TCGTGTGGCACTGACTGGGACATCGTCAGGAAGTGCATCTGTGCTGCCTATTTCCACCAA
GCAGCCAAGCTCAAGGGAATCGGGGAGTACGTGAACATCCGCACAGGGATGCCCTGCCAC
TTGCACCCCACCAGCTCCCTTTTTGGAATGGGCTACACCCCAGATTACATAGTGTATCAC
GAGTTGGTCATGACCACCAAGGAGTATATGCAGTGTGTGACCGCTGTGGACGGGGAGTGG
CTGGCGGAGCTGGGCCCCATGTTCTATAGCGTGAAACAGGCGGGCAAGTCACGGCAGGAG
AACCGTCGTCGGGCCAAAGAGGAAGCCTCTGCCATGGAGGAGGAGATGGCGCTGGCCGAG
GAGCAGCTGCGAGCCCGGCGGCAGGAGCAGGAGAAGCGCAGCCCCCTGGGCAGTGTCAGG
TCTACGAAGATCTACACTCCAGGCCGGAAAGAGCAAGGGGAGCCCATGACCCCTCGCCGC
ACGCCAGCCCGCTTTGGTCTG
Protein sequence predicted based on nucleotide sequence
MGDTSEDASIHRLEGTDLDCQVGGLICKSKSAASEQHVFKAPAPRPSLLGLDLLASLKRR EREEKDDGEDKKKSKVSSYKDWEESKDDQKDAEEEGGDQAGQNIRKDRHYRSARVETPSH PGGVSEEFWERSRQRERERREHGVYASSKEEKDWKKEKSRDRDYDRKRDRDERDRSRHSS RSERDGGSERSSRRNEPESPRHRPKDAATPSRSTWEEEDSGYGSSRRSQWESPSPTPSYR DSERSHRLSTRDRDRSVRGKYSDDTPLPTPSYKYNEWADDRRHLGSTPRLSRGRGRREEG EEGISFDTEEERQQWEDDQRQADRDWYMMDEGYDEFHNPLAYSSEDYVRRREQHLHKQKQ KRISAQRRQINEDNERWETNRMLTSGVVHRLEVDEDFEEDNAAKVHLMVHNLVPPFLDGR IVFTKQPEPVIPVKDATSDLAIIARKGSQTVRKHREQKERKKAQHKHWELAGTKLGDIMG VKKEEEPDKAVTEDGKVDYRTEQKFADHMKRKSEASSEFAKKKSILEQRQYLPIFAVQQE LLTIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTDYGMIGCTQPRRVAAMSVAKRVSEE MGGNLGEEVGYAIRFEDCTSENTLIKYMTDGILLRESLREADLDHYSAIIMDEAHERSLN TDVLFGLLREVVARRSDLKLIVTSATMDAEKFAAFFGNVPIFHIPGRTFPVDILFSKTPQ EDYVEAAVKQSLQVHLSGAPGDILIFMPGQEDIEVTSDQIVEHLEELENAPALAVLPIYS QLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGIMFVIDSGYCKLKVFNPRIGMDAL QIYPISQANANQRSGRAGRTGPGQCFRLYTQSAYKNELLTTTVPEIQRTNLANVVLLLKS LGVQDLLQFHFMDPPPEDNMLNSMYQLWILGALDNTGGLTSTGRLMVEFPLDPALSKMLI VSCDMGCSSEILLIVSMLSVPAIFYRPKGREEESDQIREKFAVPESDHLTYLNVYLQWKN NNYSTIWCNDHFIHAKAMRKVREVRAQLKDIMVQQRMSLASCGTDWDIVRKCICAAYFHQ AAKLKGIGEYVNIRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEW LAELGPMFYSVKQAGKSRQENRRRAKEEASAMEEEMALAEEQLRARRQEQEKRSPLGSVR STKIYTPGRKEQGEPMTPRRTPARFGL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
