Gene:
Gene Symbol:
SMC1B
Gene Name:
structural maintenance of chromosomes 1B
Original Clone ID:
ccsbBroadEn_11845
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC126208.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00506029
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCCCACCTGGAGCTGCTGCTTGTGGAAAATTTCAAGTCGTGGCGGGGCCGCCAGGTC
ATTGGCCCCTTCCGGAGGTTCACCTGCATCATCGGCCCCAACGGCTCTGGAAAATCTAAT
GTAATGGATGCACTTAGTTTTGTAATGGGAGAGAAAATAGCTAATTTAAGAGTGAAAAAT
ATTCAAGAACTCATTCATGGAGCACATATTGGAAAACCTATTTCTTCTTCTGCAAGTGTA
AAAATTATATATGTGGAGGAAAGTGGCGAAGAGAAAACATTTGCAAGGATTATCCGAGGG
GGATGCTCAGAATTTCGCTTTAATGATAATCTTGTGAGTCGTTCTGTTTACATTGCAGAG
TTGGAAAAGATAGGCATAATAGTCAAAGCACAAAATTGTTTGGTTTTTCAGGGAACTGTA
GAGTCAATTTCAGTGAAGAAACCCAAAGAAAGGACCCAGTTTTTTGAGGAAATCAGCACT
TCAGGAGAGCTTATAGGAGAATATGAAGAAAAGAAAAGAAAGTTACAAAAAGCCGAAGAG
GATGCACAGTTTAACTTTAATAAGAAAAAAAATATAGCGGCAGAGCGCAGACAAGCAAAA
TTAGAGAAGGAAGAGGCAGAACGTTACCAGAGTCTCCTTGAAGAACTGAAAATGAACAAG
ATACAACTGCAGCTTTTTCAACTATACCATAATGAGAAAAAGATTCATCTCCTGAACACC
AAGTTAGAGCATGTGAATAGGGATTTGAGTGTCAAAAGAGAGTCTTTGTCTCATCATGAA
AACATAGTTAAAGCCAGGAAAAAGGAACATGGAATGCTAACTAGACAACTACAACAAACA
GAAAAAGAATTAAAATCGGTTGAAACCCTTTTAAATCAGAAGAGGCCTCAGTACATTAAA
GCCAAAGAAAACACTTCTCACCACCTTAAGAAATTAGATGTGGCTAAGAAATCAATAAAG
GACAGCGAAAAACAATGTTCTAAACAGGAAGATGATATAAAAGCCCTGGAGACAGAGCTG
GCTGATTTAGATGCTGCATGGAGAAGTTTTGAAAAGCAGATTGAGGAAGAAATTTTACAT
AAAAAGCGAGACATTGAACTGGAAGCCAGTCAGCTGGATCGTTATAAAGAACTTAAGGAA
CAAGTAAGAAAGAAAGTAGCTACAATGACTCAACAACTGGAAAAACTGCAGTGGGAACAG
AAGACAGATGAAGAAAGACTGGCATTTGAAAAGAGGAGGCATGGAGAAGTTCAGGGAAAT
CTAAAACAAATAAAAGAACAAATAGAAGATCATAAAAAACGAATAGAGAAGTTAGAGGAG
TATACAAAGACATGCATGGATTGCTTGAAAGAGAAAAAACAGCAAGAGGAAACCCTAGTG
GATGAAATTGAAAAAACAAAATCAAGAATGTCTGAAGTTAATGAAGAATTGAATCTTATT
AGAAGTGAATTGCAGAATGCTGGGATTGATACCCATGAGGGAAAACGTCAGCAAAAGAGA
GCAGAGGTTCTGGAACACCTTAAAAGACTGTACCCAGATTCTGTGTTTGGAAGACTATTT
GACCTGTGTCATCCTATTCATAAGAAATACCAGCTGGCTGTTACTAAGGTTTTTGGCCGG
TTCATCACTGCCATTGTTGTAGCCTCTGAAAAGGTAGCAAAAGATTGTATTCGATTTCTG
AAGGAGGAAAGAGCTGAACCTGAGACATTCCTCGCTCTAGATTACCTTGATATCAAGCCA
ATCAATGAAAGACTAAGGGAGCTTAAAGGCTGTAAAATGGTGATTGATGTCATAAAGACT
CAGTTTCCTCAGCTGAAGAAAGTGATTCAGTTTGTGTGTGGAAATGGTCTTGTTTGTGAG
ACTATGGAAGAAGCAAGGCATATTGCACTCAGTGGACCTGAAAGACAGAAAACAGTAGCT
CTTGATGGAACATTATTTTTAAAATCTGGAGTGATCTCTGGAGGGTCAAGTGACTTAAAA
TACAAGGCTAGATGCTGGGATGAGAAAGAGTTAAAGAATCTAAGAGACAGACGAAGCCAG
AAAATCCAAGAGCTAAAGGGTTTAATGAAGACACTCCGCAAAGAAACAGATTTGAAACAA
ATACAGACCCTGATACAGGGAACTCAAACACGACTCAAATATTCACAAAATGAACTAGAG
ATGATTAAGAAGAAGCACCTTGTTGCTTTTTACCAGGAACAATCTCAGTTACAAAGTGAA
CTACTAAATATTGAGTCTCAATGTATTATGTTGAGTGAAGGAATCAAGGAACGACAACGA
AGAATTAAAGAATTTCAAGAAAAGATAGATAAGGTAGAAGACGATATCTTCCAACACTTC
TGTGAAGAAATTGGCGTGGAAAATATTCGTGAATTTGAGAACAAACATGTTAAACGGCAA
CAAGAAATTGATCAAAAAAGATTAGAATTTGAAAAACAAAAAACTCGGCTTAATGTTCAA
CTTGAGTATAGTCGCAGTCACCTTAAGAAGAAACTGAATAAGATCAACACATTAAAAGAA
ACTATCCAGAAAGGTAGTGAAGATATTGATCACCTAAAGAAGGCTGAAGAAAACTGTCTG
CAGACAGTGAATGAACTCATGGCAAAGCAGCAGCAACTTAAGGACATACGTGTCACTCAG
AACTCCAGTGCCGAGAAAGTTCAAACTCAAATTGAAGAGGAACGGAAGAAGTTTCTGGCT
GTTGATAGGGAAGTGGGGAAATTGCAAAAAGAAGTTGTAAGTATTCAAACTTCTCTGGAA
CAGAAACGATTAGAGAAGCATAACTTGCTGCTTGATTGCAAAGTGCAAGACATTGAGATA
ATCCTTTTGTCGGGGTCACTGGATGACATCATTGAAGTGGAGATGGGAACTGAAGCAGAA
AGTACCCAGGCAACAATTGATATCTATGAAAAAGAAGAAGCCTTTGAAATAGACTACAGC
TCTCTAAAAGAGGATTTGAAGGCTCTACAGTCTGATCAAGAAATCGAGGCCCACCTTAGG
CTCTTATTGCAGCAAGTAGCATCCCAGGAAGATATCTTACTGAAAACAGCAGCCCCAAAC
CTACGAGCACTGGAGAACTTAAAGACTGTCAGAGACAAGTTTCAAGAGTCCACAGATGCT
TTTGAGGCCAGCAGAAAGGAAGCCAGAATGTGTAGGCAAGAGTTCGAGCAAGTGAAAAAA
AGGAGATACGATCTTTTCACCCAGTGTTTTGAGCATGTCTCAATCTCAATTGATCAAATC
TACAAGAAGCTCTGCAGAAACAACAGCGCCCAAGTGTCAAGTTACATCAAAGAGCAAACT
CAAGACCAGTTTCAGATGATAGTCATCTCCCTAAAAGAAGAGTTCTATTCCAGAGCCGAC
GCGCTGATCGGCATCTATCCTGAGTACGATGACTGCATGTTCAGCCGAGTTTTGACCCTA
GATCTTTCTCAGTATCCAGACACTGAAGGCCAAGAAAGCAGCAAGAGACACGGAGAGTCC
CGC
Protein sequence predicted based on nucleotide sequence
MAHLELLLVENFKSWRGRQVIGPFRRFTCIIGPNGSGKSNVMDALSFVMGEKIANLRVKN IQELIHGAHIGKPISSSASVKIIYVEESGEEKTFARIIRGGCSEFRFNDNLVSRSVYIAE LEKIGIIVKAQNCLVFQGTVESISVKKPKERTQFFEEISTSGELIGEYEEKKRKLQKAEE DAQFNFNKKKNIAAERRQAKLEKEEAERYQSLLEELKMNKIQLQLFQLYHNEKKIHLLNT KLEHVNRDLSVKRESLSHHENIVKARKKEHGMLTRQLQQTEKELKSVETLLNQKRPQYIK AKENTSHHLKKLDVAKKSIKDSEKQCSKQEDDIKALETELADLDAAWRSFEKQIEEEILH KKRDIELEASQLDRYKELKEQVRKKVATMTQQLEKLQWEQKTDEERLAFEKRRHGEVQGN LKQIKEQIEDHKKRIEKLEEYTKTCMDCLKEKKQQEETLVDEIEKTKSRMSEVNEELNLI RSELQNAGIDTHEGKRQQKRAEVLEHLKRLYPDSVFGRLFDLCHPIHKKYQLAVTKVFGR FITAIVVASEKVAKDCIRFLKEERAEPETFLALDYLDIKPINERLRELKGCKMVIDVIKT QFPQLKKVIQFVCGNGLVCETMEEARHIALSGPERQKTVALDGTLFLKSGVISGGSSDLK YKARCWDEKELKNLRDRRSQKIQELKGLMKTLRKETDLKQIQTLIQGTQTRLKYSQNELE MIKKKHLVAFYQEQSQLQSELLNIESQCIMLSEGIKERQRRIKEFQEKIDKVEDDIFQHF CEEIGVENIREFENKHVKRQQEIDQKRLEFEKQKTRLNVQLEYSRSHLKKKLNKINTLKE TIQKGSEDIDHLKKAEENCLQTVNELMAKQQQLKDIRVTQNSSAEKVQTQIEEERKKFLA VDREVGKLQKEVVSIQTSLEQKRLEKHNLLLDCKVQDIEIILLSGSLDDIIEVEMGTEAE STQATIDIYEKEEAFEIDYSSLKEDLKALQSDQEIEAHLRLLLQQVASQEDILLKTAAPN LRALENLKTVRDKFQESTDAFEASRKEARMCRQEFEQVKKRRYDLFTQCFEHVSISIDQI YKKLCRNNSAQVSSYIKEQTQDQFQMIVISLKEEFYSRADALIGIYPEYDDCMFSRVLTL DLSQYPDTEGQESSKRHGESR
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
