ATP12A (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ATP12A
Gene Name:  ATPase, H+/K+ transporting, nongastric, alpha polypeptide
Original Clone ID:
ccsbBroadEn_05866
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC031609.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00506038
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3136nts
Open reading frame: 1 to 3136

ATGCACCAGAAAACCCCAGAAATTTACTCCGTGGAGCTCAGCGGAACTAAGGACATCGTG
AAAACAGACAAGGGGGATGGCAAGGAGAAGTATAGGGGTCTGAAGAACAACTGCCTGGAA
CTCAAAAAGAAAAATCACAAAGAGGAGTTTCAGAAAGAACTCCATCTGGATGACCACAAA
CTCAGCAATAGGGAATTGGAAGAGAAATATGGCACAGACATCATTATGGGTCTCTCCAGC
ACCAGAGCTGCCGAGCTCCTGGCCCGGGATGGGCCCAACTCCCTCACCCCTCCCAAGCAG
ACGCCTGAGATCGTCAAGTTCCTCAAGCAGATGGTGGGGGGGTTCTCTATCCTCCTGTGG
GTGGGCGCCTTTCTCTGTTGGATTGCATATGGGATTCAGTACTCCAGCGACAAGTCTGCA
TCCCTGAACAACGTGTACTTGGGCTGTGTGCTTGGTCTGGTGGTCATTTTAACGGGGATC
TTTGCTTATTACCAAGAGGCAAAAAGCACCAACATCATGTCCAGCTTCAATAAGATGATC
CCTCAGCAAGCTCTCGTCATCCGAGATTCCGAGAAGAAGACCATCCCTTCAGAGCAGCTG
GTGGTGGGGGACATTGTGGAGGTCAAAGGAGGAGACCAGATCCCTGCAGACATCAGGGTG
CTGTCTTCTCAGGGGTGTCGGGTGGATAACTCATCTCTCACGGGGGAGTCTGAGCCCCAG
CCCCGCTCCTCTGAGTTTACCCATGAAAACCCCCTGGAAACAAAGAACATCTGCTTCTAT
TCCACAACGTGTCTGGAAGCATCTACTTCCCCTGTAGGCACTGTCACCGGCATGGTTATC
AACACGGGTGACCGCACCATCATTGGCCATATTGCCTCATTGGCCTCAGGAGTTGGAAAT
GAGAAGACGCCCATTGCCATTGAGATCGAGCACTTTGTTCACATTGTGGCAGGAGTGGCT
GTCTCCATCGGCATCCTTTTCTTCATCATCGCTGTGTCCCTGAAGTATCAAGTCCTGGAC
TCCATCATCTTCCTCATTGGCATCATTGTGGCCAATGTGCCCGAGGGCCTCCTGGCCACT
GTCACTGTGACCCTGTCGCTGACAGCAAAACGGATGGCCAAGAAGAACTGCCTGGTGAAG
AACCTGGAGGCTGTGGAGACCCTCGGCTCCACCTCCATCATCTGCTCGGACAAGACTGGG
ACACTGACCCAGAACAGGATGACAGTGGCCCATCTGTGGTTCGACAATCAGATCTTTGTG
GCTGACACCAGTGAGGACCATTCAAACCAAGTCTTTGACCAAAGCTCTAGGACTTGGGCC
TCCTTATCCAAGATAATAACATTGTGTAACCGAGCAGAGTTCAAGCCAGGACAGGAAAAT
GTCCCCATCATGAAGAAAGCTGTGATTGGAGATGCCTCAGAAACTGCTCTTTTAAAATTC
TCAGAGGTCATTTTGGGTGATGTGATGGAAATTAGAAAAAGAAACCGCAAAGTAGCTGAA
ATCCCTTTTAACTCTACTAATAAATTTCAGCTCTCCATCCACGAGATGGATGACCCCCAC
GGCAAGCGCTTCCTCATGGTGATGAAGGGGGCCCCTGAGCGCATTCTAGAGAAATGCAGC
ACCATCATGATCAACGGCGAGGAGCACCCACTGGACAAGAGCACTGCCAAGACCTTCCAC
ACAGCCTACATGGAGCTGGGCGGGTTGGGCGAGCGTGTGCTGGGTTTCTGTCATCTCTAC
CTGCCAGCAGACGAGTTTCCAGAAACCTACTCATTTGACATAGACGCTATGAACTTTCCG
ACCTCCAACCTCTGTTTTGTGGGACTCTTGTCAATGATCGATCCCCCTCGGTCCACCGTG
CCAGATGCAGTCACCAAATGCCGGAGTGCAGGGATCAAGGTTATTATGGTTACTGGTGAT
CATCCCATCACAGCCAAAGCTATTGCCAAGAGTGTGGGGATCATTTCAGCCAACAGTGAA
ACAGTGGAAGACATTGCACATCGCCTCAACATTGCTGTGGAGCAAGTTAACAAACGGGAT
GCCAAGGCCGCTGTGGTGACTGGCATGGAGCTGAAGGACATGAGCTCAGAACAGCTGGAT
GAGATCTTAGCCAACTACCAGGAGATTGTCTTTGCCCGGACATCCCCCCAGCAGAAGCTG
ATCATTGTGGAGGGCTGTCAGAGGCAGGATGCTGTTGTTGCTGTGACCGGGGATGGAGTT
AATGACTCTCCGGCTCTAAAGAAGGCAGACATTGGGATTGCCATGGGGATAGCAGGTTCT
GATGCAGCCAAAAATGCAGCCGACATGGTCTTGCTGGACGACAACTTCGCATCCATCGTC
ACAGGGGTGGAGGAAGGTCGCCTGATCTTTGACAACCTCAAGAAGACTATTGCTTATTCC
CTGACCAAGAACATTGCCGAGCTGTGCCCCTTTCTGATCTACATCATTGTCGGGCTCCCC
CTGCCCATTGGCACCATCACCATTCTGTTCATTGACTTGGGGACAGACATTATCCCCTCC
ATTGCCTTGGCGTACGAGAAAGCTGAAAGTGACATCATGAACAGGAAGCCTCGCCACAAG
AATAAGGACAGGCTGGTGAACCAGCCGCTCGCTGTGTACTCATACCTGCACATTGGCCTC
ATGCAAGCCCTGGGAGCTTTCCTTGTGTATTTCACCGTCTATGCACAAGAGGGCTTTCTG
CCCCGCACTCTCATTAACCTGCGGGTAGAATGGGAGAAGGACTACGTGAATGACTTGAAA
GACAGCTATGGGCAGGAATGGACAAGGTACCAGAGGGAATACCTAGAATGGACGGGCTAC
ACGGCTTTCTTTGTTGGCATCCTAGTCCAGCAAATAGCAGATCTGATCATCAGGAAAACC
CGGAGGAATTCCATCTTCCAGCAGGGTCTCTTCAGAAATAAAGTCATCTGGGTGGGGATC
ACCTCACAGATCATCATTGGTCTGATCCTCTCCTATGGCCTCGGAAGTGTCACAGCCTTG
AGTTTCACCATGCTTAGGGCTCAGTACTGGTTTGTGGCTGTGCCGCACGCCATCCTGATC
TGGGTGTATGATGAGGTGCGGAAGCTCTTCATCAGGCTCTACCCTGGAAGCTGGTGGGAT
AAGAACATGTATTAT

Protein sequence predicted based on nucleotide sequence

MHQKTPEIYSVELSGTKDIVKTDKGDGKEKYRGLKNNCLELKKKNHKEEFQKELHLDDHK
LSNRELEEKYGTDIIMGLSSTRAAELLARDGPNSLTPPKQTPEIVKFLKQMVGGFSILLW
VGAFLCWIAYGIQYSSDKSASLNNVYLGCVLGLVVILTGIFAYYQEAKSTNIMSSFNKMI
PQQALVIRDSEKKTIPSEQLVVGDIVEVKGGDQIPADIRVLSSQGCRVDNSSLTGESEPQ
PRSSEFTHENPLETKNICFYSTTCLEASTSPVGTVTGMVINTGDRTIIGHIASLASGVGN
EKTPIAIEIEHFVHIVAGVAVSIGILFFIIAVSLKYQVLDSIIFLIGIIVANVPEGLLAT
VTVTLSLTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLWFDNQIFV
ADTSEDHSNQVFDQSSRTWASLSKIITLCNRAEFKPGQENVPIMKKAVIGDASETALLKF
SEVILGDVMEIRKRNRKVAEIPFNSTNKFQLSIHEMDDPHGKRFLMVMKGAPERILEKCS
TIMINGEEHPLDKSTAKTFHTAYMELGGLGERVLGFCHLYLPADEFPETYSFDIDAMNFP
TSNLCFVGLLSMIDPPRSTVPDAVTKCRSAGIKVIMVTGDHPITAKAIAKSVGIISANSE
TVEDIAHRLNIAVEQVNKRDAKAAVVTGMELKDMSSEQLDEILANYQEIVFARTSPQQKL
IIVEGCQRQDAVVAVTGDGVNDSPALKKADIGIAMGIAGSDAAKNAADMVLLDDNFASIV
TGVEEGRLIFDNLKKTIAYSLTKNIAELCPFLIYIIVGLPLPIGTITILFIDLGTDIIPS
IALAYEKAESDIMNRKPRHKNKDRLVNQPLAVYSYLHIGLMQALGAFLVYFTVYAQEGFL
PRTLINLRVEWEKDYVNDLKDSYGQEWTRYQREYLEWTGYTAFFVGILVQQIADLIIRKT
RRNSIFQQGLFRNKVIWVGITSQIIIGLILSYGLGSVTALSFTMLRAQYWFVAVPHAILI
WVYDEVRKLFIRLYPGSWWDKNMYY

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable