DNASU Plasmid | NKIRAS2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: NKIRAS2

Gene Name: NFKB inhibitor interacting Ras-like 2

Original Clone ID:

ccsbBroadEn_03040

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC007450.2,BC063498.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00506785

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 574nts

Open reading frame: 1 to 574

Reference Sequence Alignment

ATGGGGAAGAGCTGCAAGGTGGTCGTGTGTGGCCAGGCGTCTGTGGGCAAAACTTCAATC
CTGGAGCAGCTTCTGTATGGGAACCATGTAGTGGGTTCGGAGATGATCGAGACGCAGGAG
GACATCTACGTGGGCTCCATTGAGACAGACCGGGGGGTGCGAGAGCAGGTGCGTTTCTAT
GACACCCGGGGGCTCCGAGATGGGGCCGAACTGCCCCGACACTGCTTCTCTTGCACTGAT
GGCTACGTCCTGGTCTATAGCACAGATAGCAGAGAGTCTTTTCAGCGTGTGGAGCTGCTC
AAGAAGGAGATTGACAAATCCAAGGACAAGAAGGAGGTCACCATCGTGGTCCTTGGCAAC
AAGTGTGACTTACAGGAGCAGCGGCGTGTAGACCCAGATGTGGCTCAGCACTGGGCCAAG
TCAGAGAAGGTGAAGCTGTGGGAGGTGTCAGTGGCGGACCGGCGCTCCCTCCTGGAGCCC
TTTGTCTACTTGGCCAGCAAGATGACGCAACCCCAGAGCAAGTCTGCCTTCCCCCTCAGC
CGGAAGAACAAGGGCAGCGGCTCCTTGGATGGC

Protein sequence predicted based on nucleotide sequence

MGKSCKVVVCGQASVGKTSILEQLLYGNHVVGSEMIETQEDIYVGSIETDRGVREQVRFY
DTRGLRDGAELPRHCFSCTDGYVLVYSTDSRESFQRVELLKKEIDKSKDKKEVTIVVLGN
KCDLQEQRRVDPDVAQHWAKSEKVKLWEVSVADRRSLLEPFVYLASKMTQPQSKSAFPLS
RKNKGSGSLDG

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

NKIRAS2

Symbol Nomenclature :

NKIRAS2

SYNONYM :

KBRAS2; kappaB-Ras2

Designation :

I-kappa-B-interacting Ras-like protein 2|NF-kappa-B inhibitor-interacting Ras-like protein 2|NFKB inhibitor interacting Ras-like protein 2|kappa B-Ras protein 2

Full Nomenclature :

NFKB inhibitor interacting Ras-like 2

GENEID :

28511

GenBank Accession :

DQ896563

HGNC :

17898

MIM :

604497

Vega :

OTTHUMG00000133503

Target GenBank:

DQ896563

Reference Sequence Alignment

  

HsCD00506785        ATGGGGAAGAGCTGCAAGGTGGTCGTGTGTGGCCAGGCGTCTGTGGGCAAAACTTCAATC
XM_005257251.1      ATGGGGAAGAGCTGCAAGGTGGTCGTGTGTGGCCAGGCGTCTGTGGGCAAAACTTCAATC
NM_017595.5         ATGGGGAAGAGCTGCAAGGTGGTCGTGTGTGGCCAGGCGTCTGTGGGCAAAACTTCAATC
NM_001001349.2      ATGGGGAAGAGCTGCAAGGTGGTCGTGTGTGGCCAGGCGTCTGTGGGCAAAACTTCAATC
NM_001144927.1      ATGGGGAAGAGCTGCAAGGTGGTCGTGTGTGGCCAGGCGTCTGTGGGCAAAACTTCAATC
                    ************************************************************

HsCD00506785        CTGGAGCAGCTTCTGTATGGGAACCATGTAGTGGGTTCGGAGATGATCGAGACGCAGGAG
XM_005257251.1      CTGGAGCAGCTTCTGTATGGGAACCATGTAGTGGGTTCGGAGATGATCGAGACGCAGGAG
NM_017595.5         CTGGAGCAGCTTCTGTATGGGAACCATGTAGTGGGTTCGGAGATGATCGAGACGCAGGAG
NM_001001349.2      CTGGAGCAGCTTCTGTATGGGAACCATGTAGTGGGTTCGGAGATGATCGAGACGCAGGAG
NM_001144927.1      CTGGAGCAGCTTCTGTATGGGAACCATGTAGTGGGTTCGGAGATGATCGAGACGCAGGAG
                    ************************************************************

HsCD00506785        GACATCTACGTGGGCTCCATTGAGACAGACCGGGGGGTGCGAGAGCAGGTGCGTTTCTAT
XM_005257251.1      GACATCTACGTGGGCTCCATTGAGACAGACCGGGGGGTGCGAGAGCAGGTGCGTTTCTAT
NM_017595.5         GACATCTACGTGGGCTCCATTGAGACAGACCGGGGGGTGCGAGAGCAGGTGCGTTTCTAT
NM_001001349.2      GACATCTACGTGGGCTCCATTGAGACAGACCGGGGGGTGCGAGAGCAGGTGCGTTTCTAT
NM_001144927.1      GACATCTACGTGGGCTCCATTGAGACAGACCGGGGGGTGCGAGAGCAGGTGCGTTTCTAT
                    ************************************************************

HsCD00506785        GACACCCGGGGGCTCCGAGATGGGGCCGAACTGCCCCGACACTGCTTCTCTTGCACTGAT
XM_005257251.1      GACACCCGGGGGCTCCGAGATGGGGCCGAACTGCCCCGACACTGCTTCTCTTGCACTGAT
NM_017595.5         GACACCCGGGGGCTCCGAGATGGGGCCGAACTGCCCCGACACTGCTTCTCTTGCACTGAT
NM_001001349.2      GACACCCGGGGGCTCCGAGATGGGGCCGAACTGCCCCGACACTGCTTCTCTTGCACTGAT
NM_001144927.1      GACACCCGGGGGCTCCGAGATGGGGCCGAACTGCCCCGACACTGCTTCTCTTGCACTGAT
                    ************************************************************

HsCD00506785        GGCTACGTCCTGGTCTATAGCACAGATAGCAGAGAGTCTTTTCAGCGTGTGGAGCTGCTC
XM_005257251.1      GGCTACGTCCTGGTCTATAGCACAGATAGCAGAGAGTCTTTTCAGCGTGTGGAGCTGCTC
NM_017595.5         GGCTACGTCCTGGTCTATAGCACAGATAGCAGAGAGTCTTTTCAGCGTGTGGAGCTGCTC
NM_001001349.2      GGCTACGTCCTGGTCTATAGCACAGATAGCAGAGAGTCTTTTCAGCGTGTGGAGCTGCTC
NM_001144927.1      GGCTACGTCCTGGTCTATAGCACAGATAGCAGAGAGTCTTTTCAGCGTGTGGAGCTGCTC
                    ************************************************************

HsCD00506785        AAGAAGGAGATTGACAAATCCAAGGACAAGAAGGAGGTCACCATCGTGGTCCTTGGCAAC
XM_005257251.1      AAGAAGGAGATTGACAAATCCAAGGACAAGAAGGAGGTCACCATCGTGGTCCTTGGCAAC
NM_017595.5         AAGAAGGAGATTGACAAATCCAAGGACAAGAAGGAGGTCACCATCGTGGTCCTTGGCAAC
NM_001001349.2      AAGAAGGAGATTGACAAATCCAAGGACAAGAAGGAGGTCACCATCGTGGTCCTTGGCAAC
NM_001144927.1      AAGAAGGAGATTGACAAATCCAAGGACAAGAAGGAGGTCACCATCGTGGTCCTTGGCAAC
                    ************************************************************

HsCD00506785        AAGTGTGACTTACAGGAGCAGCGGCGTGTAGACCCAGATGTGGCTCAGCACTGGGCCAAG
XM_005257251.1      AAGTGTGACTTACAGGAGCAGCGGCGTGTAGACCCAGATGTGGCTCAGCACTGGGCCAAG
NM_017595.5         AAGTGTGACTTACAGGAGCAGCGGCGTGTAGACCCAGATGTGGCTCAGCACTGGGCCAAG
NM_001001349.2      AAGTGTGACTTACAGGAGCAGCGGCGTGTAGACCCAGATGTGGCTCAGCACTGGGCCAAG
NM_001144927.1      AAGTGTGACTTACAGGAGCAGCGGCGTGTAGACCCAGATGTGGCTCAGCACTGGGCCAAG
                    ************************************************************

HsCD00506785        TCAGAGAAGGTGAAGCTGTGGGAGGTGTCAGTGGCGGACCGGCGCTCCCTCCTGGAGCCC
XM_005257251.1      TCAGAGAAGGTGAAGCTGTGGGAGGTGTCAGTGGCGGACCGGCGCTCCCTCCTGGAGCCC
NM_017595.5         TCAGAGAAGGTGAAGCTGTGGGAGGTGTCAGTGGCGGACCGGCGCTCCCTCCTGGAGCCC
NM_001001349.2      TCAGAGAAGGTGAAGCTGTGGGAGGTGTCAGTGGCGGACCGGCGCTCCCTCCTGGAGCCC
NM_001144927.1      TCAGAGAAGGTGAAGCTGTGGGAGGTGTCAGTGGCGGACCGGCGCTCCCTCCTGGAGCCC
                    ************************************************************

HsCD00506785        TTTGTCTACTTGGCCAGCAAGATGACGCAACCCCAGAGCAAGTCTGCCTTCCCCCTCAGC
XM_005257251.1      TTTGTCTACTTGGCCAGCAAGATGACGCAACCCCAGAGCAAGTCTGCCTTCCCCCTCAGC
NM_017595.5         TTTGTCTACTTGGCCAGCAAGATGACGCAACCCCAGAGCAAGTCTGCCTTCCCCCTCAGC
NM_001001349.2      TTTGTCTACTTGGCCAGCAAGATGACGCAACCCCAGAGCAAGTCTGCCTTCCCCCTCAGC
NM_001144927.1      TTTGTCTACTTGGCCAGCAAGATGACGCAACCCCAGAGCAAGTCTGCCTTCCCCCTCAGC
                    ************************************************************

HsCD00506785        CGGAAGAACAAGGGCAGCGGCTCCTTGGATGGC---
XM_005257251.1      CGGAAGAACAAGGGCAGCGGCTCCTTGGATGGCTGA
NM_017595.5         CGGAAGAACAAGGGCAGCGGCTCCTTGGATGGCTGA
NM_001001349.2      CGGAAGAACAAGGGCAGCGGCTCCTTGGATGGCTGA
NM_001144927.1      CGGAAGAACAAGGGCAGCGGCTCCTTGGATGGCTGA
                    *********************************