Gene:
Original Clone ID:
ccsbBroadEn_06831
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
Original GenBank Accession: BC004247.1
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration
HsCD00506786
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGCAGGCCATCAAGTGTGTGGTGGTGGGAGACGGAGCTGTAGGTAAAACTTGCCTACTG
ATCAGTTACACAACCAATGCATTTCCTGGAGAATATATCCCTACTGTCTTTGACAATTAT
TCTGCCAATGTTATGGTAGATGGAAAACCGGTGAATCTGGGCTTATGGGATACAGCTGGA
CAAGAAGATTATGACAGATTACGCCCCCTATCCTATCCGCAAACAGATGTGTTCTTAATT
TGCTTTTCCCTTGTGAGTCCTGCATCATTTGAAAATGTCCGTGCAAAGTGGTATCCTGAG
GTGCGGCACCACTGTCCCAACACTCCCATCATCCTAGTGGGAACTAAACTTGATCTTAGG
GATGATAAAGACACGATCGAGAAACTGAAGGAGAAGAAGCTGATTCCCATCACCTATCCG
CAGGGTCTAGCCATGGCTAAGGAGATTGGTGCTGTAAAATACCTGGAGTGCTCGGCGCTC
ACACAGCGAGGCCTCAAGACAGTGTTTGACGAAGCGATCCGAGCAGTCCTCTGCCCGCCT
CCCGTGAAGAAGAGGAAGAGAAAATGCCTGCTGTTG
Protein sequence predicted based on nucleotide sequence
MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAG QEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLR DDKDTIEKLKEKKLIPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPP PVKKRKRKCLLL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Verified by the ORFeome collaboration
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
Gene
Gene Symbol :
RAC1
Symbol Nomenclature :
RAC1
SYNONYM :
Rac-1; TC-25; p21-Rac1
Designation :
cell migration-inducing gene 5 protein|ras-like protein TC25|ras-related C3 botulinum toxin substrate 1
Full Nomenclature :
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
GENEID :
Locus Tag :
MIG5
GenBank Accession :
HGNC :
MIM :
Vega :
Target GenBank:
Reference Sequence Alignment
HsCD00506786 ATGCAGGCCATCAAGTGTGTGGTGGTGGGAGACGGAGCTGTAGGTAAAACTTGCCTACTG NM_005052.2 ATGCAGGCCATCAAGTGCGTGGTGGTCGGCGACGGCGCCGTGGGGAAGACATGCTTGCTG NM_006908.4 ATGCAGGCCATCAAGTGTGTGGTGGTGGGAGACGGAGCTGTAGGTAAAACTTGCCTACTG NM_002872.4 ATGCAGGCCATCAAGTGTGTGGTGGTGGGAGATGGGGCCGTGGGCAAGACCTGCCTTCTC ***************** ******** **.** ** ** **.** **.** *** * ** HsCD00506786 ATCAGTTACACAACCAATGCATTTCCTGGAGAATATATCCCTACTGTCTTTGACAATTAT NM_005052.2 ATCAGCTACACGACCAACGCCTTCCCCGGAGAGTACATCCCCACCGTTTTTGACAACTAC NM_006908.4 ATCAGTTACACAACCAATGCATTTCCTGGAGAATATATCCCTACTGTCTTTGACAATTAT NM_002872.4 ATCAGCTACACCACCAACGCCTTTCCCGGAGAGTACATCCCCACCGTGTTTGACAACTAT ***** ***** ***** **.** ** *****.** ***** ** ** ******** ** HsCD00506786 TCTGCCAATGTTATGGTAGATGGAAAACCGGTGAATCTGGGCTTATGGGATACAGCTGGA NM_005052.2 TCTGCCAACGTGATGGTGGACGGGAAACCAGTCAACTTGGGGCTGTGGGACACAGCGGGT NM_006908.4 TCTGCCAATGTTATGGTAGATGGAAAACCGGTGAATCTGGGCTTATGGGATACAGCTGGA NM_002872.4 TCAGCCAATGTGATGGTGGACAGCAAGCCAGTGAACCTGGGGCTGTGGGACACTGCTGGG **:***** ** *****.** .* **.**.** ** **** *.***** **:** ** HsCD00506786 CAAGAAGATTATGACAGATTACGCCCCCTATCCTATCCGCAAACAGATGTGTTCTTAATT NM_005052.2 CAGGAGGACTACGATCGGCTGCGGCCACTCTCCTACCCCCAAACTGACGTCTTTCTGATC NM_006908.4 CAAGAAGATTATGACAGATTACGCCCCCTATCCTATCCGCAAACAGATGTGTTCTTAATT NM_002872.4 CAGGAGGACTACGACCGTCTCCGGCCGCTCTCCTATCCACAGACGGACGTCTTCCTCATC **.**.** ** ** .* * ** ** **.***** ** **.** ** ** ** * ** HsCD00506786 TGCTTTTCCCTTGTGAGTCCTGCATCATTTGAAAATGTCCGTGCAAAGTGGTATCCTGAG NM_005052.2 TGCTTCTCTCTGGTGAGCCCGGCCTCCTTCGAGAATGTTCGTGCCAAGTGGTACCCGGAG NM_006908.4 TGCTTTTCCCTTGTGAGTCCTGCATCATTTGAAAATGTCCGTGCAAAGTGGTATCCTGAG NM_002872.4 TGCTTCTCCCTCGTCAGCCCAGCCTCTTATGAGAACGTCCGCGCCAAGTGGTTCCCAGAA ***** ** ** ** ** ** **.** *: **.** ** ** **.*******: ** **. HsCD00506786 GTGCGGCACCACTGTCCCAACACTCCCATCATCCTAGTGGGAACTAAACTTGATCTTAGG NM_005052.2 GTGCGGCACCACTGCCCCCACACGCCCATCCTCCTGGTGGGCACCAAGCTGGACCTCCGC NM_006908.4 GTGCGGCACCACTGTCCCAACACTCCCATCATCCTAGTGGGAACTAAACTTGATCTTAGG NM_002872.4 GTGCGGCACCACTGCCCCAGCACACCCATCATCCTGGTGGGCACCAAGCTGGACCTGCGG ************** ***..*** ******.****.*****.** **.** ** ** .* HsCD00506786 GATGATAAAGACACGATCGAGAAACTGAAGGAGAAGAAGCTGATTCCCATCACCTATCCG NM_005052.2 GACGACAAGGACACCATTGAGCGGCTGCGGGACAAGAAGCTGGCACCCATCACCTACCCA NM_006908.4 GATGATAAAGACACGATCGAGAAACTGAAGGAGAAGAAGCTGACTCCCATCACCTATCCG NM_002872.4 GACGACAAGGACACCATCGAGAAACTGAAGGAGAAGAAGCTGGCTCCCATCACCTACCCG ** ** **.***** ** ***...***..*** *********. :*********** **. HsCD00506786 CAGGGTCTAGCCATGGCTAAGGAGATTGGTGCTGTAAAATACCTGGAGTGCTCGGCGCTC NM_005052.2 CAGGGCCTGGCCATGGCCCGGGAGATTGGCTCTGTGAAATACCTGGAGTGCTCAGCCCTG NM_006908.4 CAGGGTCTAGCCATGGCTAAGGAGATTGGTGCTGTAAAATACCTGGAGTGCTCGGCGCTC NM_002872.4 CAGGGCCTGGCACTGGCCAAGGAGATTGACTCGGTGAAATACCTGGAGTGCTCAGCTCTC ***** **.**..**** ..********. * **.*****************.** ** HsCD00506786 ACACAGCGAGGCCTCAAGACAGTGTTTGACGAAGCGATCCGAGCAGTCCTCTGCCCGCCT NM_005052.2 ACCCAGCGGGGCCTGAAGACAGTGTTTGACGAGGCGATCCGCGCGGTGCTCTGCCCGCCC NM_006908.4 ACACAGCGAGGCCTCAAGACAGTGTTTGACGAAGCGATCCGAGCAGTCCTCTGCCCGCCT NM_002872.4 ACCCAGAGAGGCCTGAAAACCGTGTTCGACGAGGCCATCCGGGCCGTGCTGTGCCCTCAG **.***.*.***** **.**.***** *****.** ***** ** ** ** ***** *. HsCD00506786 CCCGTGAAGAAGAGGAAGAGAAAATGCCTGCTGTTG--- NM_005052.2 CCAGTGAAGAAGCCGGGGAAGAAGTGCACCGTCTTCTAG NM_006908.4 CCCGTGAAGAAGAGGAAGAGAAAATGCCTGCTGTTGTAA NM_002872.4 CCCACGCGGCAGCAGAAGCGCGCCTGCAGCCTCCTCTAG **.. *..*.**. *..*.. .. ***. * *
Pathways
NCI :
Alpha4 beta1 integrin signaling events
NCI :
Alpha9 beta1 integrin signaling events
NCI :
Angiopoietin receptor Tie2-mediated signaling
NCI :
Arf1 pathway
NCI :
Arf6 downstream pathway
NCI :
BCR signaling pathway
NCI :
CDC42 signaling events
NCI :
Class I PI3K signaling events
NCI :
CXCR4-mediated signaling events
NCI :
E-cadherin signaling in keratinocytes
NCI :
E-cadherin signaling in the nascent adherens junction
NCI :
Endothelins
NCI :
EPHA2 forward signaling
NCI :
EPHB forward signaling
NCI :
Ephrin B reverse signaling
NCI :
ErbB1 downstream signaling
NCI :
ErbB2/ErbB3 signaling events
NCI :
IL2 signaling events mediated by PI3K
NCI :
IL6-mediated signaling events
NCI :
Integrin-linked kinase signaling
NCI :
Integrins in angiogenesis
NCI :
Lissencephaly gene (LIS1) in neuronal migration and development
NCI :
LPA receptor mediated events
NCI :
mTOR signaling pathway
NCI :
N-cadherin signaling events
NCI :
Nectin adhesion pathway
NCI :
Nephrin/Neph1 signaling in the kidney podocyte
NCI :
Netrin-mediated signaling events
NCI :
Neurotrophic factor-mediated Trk receptor signaling
NCI :
Noncanonical Wnt signaling pathway
NCI :
Nongenotropic Androgen signaling
NCI :
Osteopontin-mediated events
NCI :
p38 MAPK signaling pathway
NCI :
p75(NTR)-mediated signaling
NCI :
PDGFR-beta signaling pathway
NCI :
Posttranslational regulation of adherens junction stability and dissassembly
NCI :
RAC1 signaling pathway
NCI :
Regulation of p38-alpha and p38-beta
NCI :
Regulation of RAC1 activity
NCI :
S1P1 pathway
NCI :
S1P2 pathway
NCI :
S1P3 pathway
NCI :
Signaling events mediated by focal adhesion kinase
NCI :
Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI :
Signaling events mediated by PRL
NCI :
Signaling events regulated by Ret tyrosine kinase
NCI :
Syndecan-4-mediated signaling events
NCI :
Thromboxane A2 receptor signaling
NCI :
Urokinase-type plasminogen activator (uPA) and uPAR-mediated signaling
Panther :
Axon guidance mediated by netrin
Panther :
Axon guidance mediated by semaphorins
Panther :
Axon guidance mediated by Slit/Robo
Panther :
B cell activation
Panther :
Cytoskeletal regulation by Rho GTPase
Panther :
EGF receptor signaling pathway
Panther :
FGF signaling pathway
Panther :
Huntington disease
Panther :
Inflammation mediated by chemokine and cytokine signaling pathway
Panther :
Integrin signalling pathway
Panther :
Ras Pathway
Panther :
T cell activation
Panther :
VEGF signaling pathway
Reactome :
Activation of Rac
Reactome :
Adaptive Immune System
Reactome :
Axon guidance
Reactome :
beta-catenin independent WNT signaling
Reactome :
CD28 co-stimulation
Reactome :
CD28 dependent Vav1 pathway
Reactome :
Cell death signalling via NRAGE, NRIF and NADE
Reactome :
Cell-Cell communication
Reactome :
Costimulation by the CD28 family
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
DCC mediated attractive signaling
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
DSCAM interactions
Reactome :
Factors involved in megakaryocyte development and platelet production
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
FCERI mediated MAPK activation
Reactome :
Fcgamma receptor (FCGR) dependent phagocytosis
Reactome :
G alpha (12/13) signalling events
Reactome :
GPCR downstream signaling
Reactome :
GPVI-mediated activation cascade
Reactome :
Hemostasis
Reactome :
HIV Infection
Reactome :
Host Interactions of HIV factors
Reactome :
Immune System
Reactome :
Inactivation of Cdc42 and Rac
Reactome :
Innate Immune System
Reactome :
L1CAM interactions
Reactome :
Membrane Trafficking
Reactome :
Nef and signal transduction
Reactome :
Netrin-1 signaling
Reactome :
NRAGE signals death through JNK
Reactome :
p75 NTR receptor-mediated signalling
Reactome :
PCP/CE pathway
Reactome :
Platelet activation, signaling and aggregation
Reactome :
Regulation of actin dynamics for phagocytic cup formation
Reactome :
Sema3A PAK dependent Axon repulsion
Reactome :
SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion
Reactome :
Sema4D in semaphorin signaling
Reactome :
Sema4D induced cell migration and growth-cone collapse
Reactome :
Sema4D mediated inhibition of cell attachment and migration
Reactome :
Semaphorin interactions
Reactome :
Signal Transduction
Reactome :
Signal transduction by L1
Reactome :
Signaling by GPCR
Reactome :
Signaling by Robo receptor
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by Wnt
Reactome :
Signalling by NGF
Reactome :
The role of Nef in HIV-1 replication and disease pathogenesis
Reactome :
Translocation of GLUT4 to the plasma membrane
WikiPathway :
Alpha 6 Beta 4 signaling pathway
WikiPathway :
Androgen receptor signaling pathway
WikiPathway :
angiogenesis overview
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
DNA damage response (only ATM dependent)
WikiPathway :
EGF/EGFR Signaling Pathway
WikiPathway :
Focal Adhesion
WikiPathway :
G13 Signaling Pathway
WikiPathway :
IL-6 signaling pathway
WikiPathway :
Insulin Signaling
WikiPathway :
Integrated Breast Cancer Pathway
WikiPathway :
Integrin-mediated cell adhesion
WikiPathway :
Leptin signaling pathway
WikiPathway :
MAPK signaling pathway
WikiPathway :
MicroRNAs in cardiomyocyte hypertrophy
WikiPathway :
NOD pathway
WikiPathway :
p38 MAPK Signaling Pathway
WikiPathway :
Physiological and Pathological Hypertrophy of the Heart
WikiPathway :
Prolactin Signaling Pathway
WikiPathway :
RANKL/RANK Signaling Pathway
WikiPathway :
Regulation of Actin Cytoskeleton
WikiPathway :
Regulation of toll-like receptor signaling pathway
WikiPathway :
TGF beta Signaling Pathway
WikiPathway :
TNF alpha Signaling Pathway
WikiPathway :
Toll-like receptor signaling pathway
WikiPathway :
TOR signaling
WikiPathway :
TWEAK Signaling Pathway
WikiPathway :
Wnt Signaling Pathway
Protein
SMART domain :
SMART domain :
SMART domain :
HPRD :
03627
Clone
Cloning Information :
Original Clone ID :
ccsbBroadEn_06831
Organism
Species Specific ID:
Genome
Reagents
Labome :