DNASU Plasmid | UBL4B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: UBL4B

Gene Name: ubiquitin-like 4B

Original Clone ID:

ccsbBroadEn_05130

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

Original GenBank Accession: BC058929.1

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Harvard Medical School
Dana-Farber Cancer Institute
Broad Institute of Harvard and Massachusetts Institute of Technology (MIT)
David E Hill
David E Root
The ORFeome Collaboration

HsCD00507238

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 523nts

Open reading frame: 1 to 523

Reference Sequence Alignment

ATGTTCCTCACAGTCAAGCTGCTCCTGGGCCAGAGATGCAGTCTGAAGGTGTCAGGGCAA
GAGAGTGTAGCCACGCTGAAGAGACTGGTGTCCAGGCGGCTGAAGGTGCCTGAGGAGCAG
CAGCACCTGCTTTTCCGTGGCCAGCTCCTGGAGGATGACAAGCACCTCTCTGACTACTGC
ATTGGGCCCAATGCCTCTATCAATGTCATCATGCAGCCCTTGGAGAAGATGGCGCTAAAG
GAGGCCCACCAGCCGCAGACCCAGCCCCTGTGGCACCAGCTGGGACTGGTCCTAGCTAAA
CACTTTGAACCACAGGATGCCAAGGCCGTGCTGCAGCTGCTAAGGCAGGAGCACGAGGAG
CGCCTGCAGAAGATAAGCCTGGAGCACCTGGAGCAGCTGGCCCAGTACCTCCTGGCAGAG
GAGCCTCACGTGGAGCCAGCTGGAGAGAGGGAGCTTGAGGCGAAGGCACGGCCTCAGAGC
TCCTGTGACATGGAGGAGAAGGAGGAGGCAGCAGCTGATCAG

Protein sequence predicted based on nucleotide sequence

MFLTVKLLLGQRCSLKVSGQESVATLKRLVSRRLKVPEEQQHLLFRGQLLEDDKHLSDYC
IGPNASINVIMQPLEKMALKEAHQPQTQPLWHQLGLVLAKHFEPQDAKAVLQLLRQEHEE
RLQKISLEHLEQLAQYLLAEEPHVEPAGERELEAKARPQSSCDMEEKEEAAADQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Verified by the ORFeome collaboration

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

UBL4B

Symbol Nomenclature :

UBL4B

Designation :

ubiquitin-like protein 4B

Full Nomenclature :

ubiquitin-like 4B

GENEID :

164153

GenBank Accession :

HQ448068

HGNC :

32309

MIM :

611127

Vega :

OTTHUMG00000166989

Target GenBank:

HQ448068

Reference Sequence Alignment

  

HsCD00507238      ATGTTCCTCACAGTCAAGCTGCTCCTGGGCCAGAGATGCAGTCTGAAGGTGTCAGGGCAA
NM_203412.1       ATGTTCCTCACAGTCAAGCTGCTCCTGGGCCAGAGATGCAGTCTGAAGGTGTCAGGGCAA
                  ************************************************************

HsCD00507238      GAGAGTGTAGCCACGCTGAAGAGACTGGTGTCCAGGCGGCTGAAGGTGCCTGAGGAGCAG
NM_203412.1       GAGAGTGTAGCCACGCTGAAGAGACTGGTGTCCAGGCGGCTGAAGGTGCCTGAGGAGCAG
                  ************************************************************

HsCD00507238      CAGCACCTGCTTTTCCGTGGCCAGCTCCTGGAGGATGACAAGCACCTCTCTGACTACTGC
NM_203412.1       CAGCACCTGCTTTTCCGTGGCCAGCTCCTGGAGGATGACAAGCACCTCTCTGACTACTGC
                  ************************************************************

HsCD00507238      ATTGGGCCCAATGCCTCTATCAATGTCATCATGCAGCCCTTGGAGAAGATGGCGCTAAAG
NM_203412.1       ATTGGGCCCAATGCCTCTATCAATGTCATCATGCAGCCCTTGGAGAAGATGGCGCTAAAG
                  ************************************************************

HsCD00507238      GAGGCCCACCAGCCGCAGACCCAGCCCCTGTGGCACCAGCTGGGACTGGTCCTAGCTAAA
NM_203412.1       GAGGCCCACCAGCCGCAGACCCAGCCCCTGTGGCACCAGCTGGGACTGGTCCTAGCTAAA
                  ************************************************************

HsCD00507238      CACTTTGAACCACAGGATGCCAAGGCCGTGCTGCAGCTGCTAAGGCAGGAGCACGAGGAG
NM_203412.1       CACTTTGAACCACAGGATGCCAAGGCCGTGCTGCAGCTGCTAAGGCAGGAGCACGAGGAG
                  ************************************************************

HsCD00507238      CGCCTGCAGAAGATAAGCCTGGAGCACCTGGAGCAGCTGGCCCAGTACCTCCTGGCAGAG
NM_203412.1       CGCCTGCAGAAGATAAGCCTGGAGCACCTGGAGCAGCTGGCCCAGTACCTCCTGGCAGAG
                  ************************************************************

HsCD00507238      GAGCCTCACGTGGAGCCAGCTGGAGAGAGGGAGCTTGAGGCGAAGGCACGGCCTCAGAGC
NM_203412.1       GAGCCTCACGTGGAGCCAGCTGGAGAGAGGGAGCTTGAGGCGAAGGCACGGCCTCAGAGC
                  ************************************************************

HsCD00507238      TCCTGTGACATGGAGGAGAAGGAGGAGGCAGCAGCTGATCAG---
NM_203412.1       TCCTGTGACATGGAGGAGAAGGAGGAGGCAGCAGCTGATCAGTAA
                  ******************************************